连续离子交换法分离发酵液中的L-精氨酸

发布时间：2018-03-30 00:03

本文选题：发酵液　切入点：L-精氨酸　出处：《食品与发酵工业》2017年06期

【摘要】：相比传统的固定床离子交换提取工艺,连续离子交换工艺分离发酵液中L-精氨酸具有连续、稳定、高效等优点。钝齿棒杆菌是1株具有自有知识产权的高产L-精氨酸工业用菌株,产量达74.5 g/L。该研究通过静态吸附及解吸实验,筛选得到最优树脂D155,可在30 min内达到吸附平衡,平衡吸附量达121.31 mg/g。通过固定床实验,确定了最佳进料流速为6 mL/min,穿透时间为29 min,半饱和时间为59 min,饱和时间86 min,穿透吸附容量为134.7 mg/g,半饱和吸附容量为193.8 mg/g,饱和吸附容量为261.2 mg/g,为最佳洗脱剂为2 mol/L的氨水,洗脱率为97.14%。通过30柱连续离子交换实验,确定了交换区、交换后水洗区、洗脱区、洗脱后水洗区、顶水区最佳进料流速分别为6、6、5、10、3 mL/min。在各区最佳进料流速下,各出口浓度呈周期性变化。该工艺提高了L-精氨酸分离工艺的连续性、稳定性及分离效率,有效地降低了分离过程中的耗水量、耗碱量及劳动力投入,为发酵生产L-精氨酸提供了更科学有效的分离工艺。
[Abstract]:Compared with the traditional fixed-bed ion exchange extraction process, the separation of L-arginine from fermentation broth by continuous ion exchange process is continuous and stable. High efficiency and so on. Corynebacterium obtuse is a high-yield L-arginine industrial strain with its own intellectual property rights. The yield is 74.5 g / L. the optimum resin D155 was obtained by static adsorption and desorption experiments, and the adsorption equilibrium could be achieved within 30 min. The equilibrium adsorption capacity is 121.31 mg / g. The optimum feed flow rate is 6 mL / min, the penetration time is 29 min, the half saturation time is 59 min, the saturation time is 86 min, the penetrating adsorption capacity is 134.7 mg / g, the half saturated adsorption capacity is 193.8 mg / g, the saturated adsorption capacity is 261.2 mg / g, and the optimal eluant is ammonia water with 2 mol/L. The elution rate is 97.14. Through the continuous ion exchange experiment of 30 columns, the exchange zone, the water washing area after exchange, the elution zone, the washing area after elution, the top water area and the top water area are determined as the best feed flow rate of 3 mL / min, respectively. The process improves the continuity, stability and separation efficiency of the L-arginine separation process, and effectively reduces the water consumption, alkali consumption and labor input during the separation process. It provides a more scientific and effective separation process for producing L-arginine by fermentation.
【作者单位】：江南大学工业生物技术教育部重点实验室;江南大学生物工程学院;
【基金】：国家高技术研究发展计划(863计划)(2015AA021004) 国家自然科学基金(31300028) 江苏省自然科学基金(BK20150002;BK20130137) 教育部重点研究项目(113033A)
【分类号】：TQ028.33;TQ922

【相似文献】