高效液相色谱法同时测定塑料和纸质包装材料中6种荧光增白剂

发布时间：2018-05-11 21:14

本文选题：高效液相色谱 + 荧光检测　；参考：《色谱》2017年11期

【摘要】：建立了同时测定塑料和纸质食品包装材料中6种脂溶性荧光增白剂(FWA 135、FWA 184、FWA 185、FWA199、FWA 378和FWA 393)的高效液相色谱方法。用三氯甲烷-乙腈(3∶7,v/v)混合溶液提取,经HLB小柱净化后用高效液相色谱-荧光检测法进行定性定量分析。采用Phenomenex C18色谱柱分离,以5 mmol/L的乙酸铵水溶液和乙腈为流动相,进行梯度洗脱。结果显示:FWA 393在15~1500μg/L范围内的线性关系良好,其余5种荧光增白剂在5~500μg/L范围内线性关系良好,相关系数均大于0.999;加标回收率为80.4%~125.0%;相对标准偏差(RSD,n=6)为1%~13%。应用该方法分析了市场销售的12个样品以验证方法的实用性。该方法前处理简单,回收率高,精密度好,适用于食品包装材料中6种荧光增白剂的检测。
[Abstract]:A high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of six liposoluble fluorescent brighteners in plastics and paper food packaging materials (FWA135FWA184FWA185FWA185FWA378 and FWA 393). The mixture solution of trichloromethane and acetonitrile (3: 7 v / v) was extracted and purified by HLB column and then analyzed qualitatively and quantitatively by high performance liquid chromatography-fluorescence detection. A Phenomenex C18 column was used to separate, and the mobile phase of 5 mmol/L ammonium acetate solution and acetonitrile was used as the mobile phase for gradient elution. The results showed that the linear range of 1: FWA 393 was 1 500 渭 g / L, the other five fluorescent brighteners had a good linear relationship in the range of 5 渭 g / L, the correlation coefficients were all greater than 0. 999, the recovery rate was 84. 4 渭 g / L, and the relative standard deviation was 1 13. This method is used to analyze 12 samples sold on the market to verify the practicability of the method. The method is simple in pretreatment, high in recovery and good in precision. It is suitable for the detection of 6 fluorescent brighteners in food packaging materials.
【作者单位】：安徽省食品药品检验研究院;安徽农业大学茶与食品科技学院;安徽农业大学省部共建茶树生物学与资源利用国家重点实验室;
【基金】：安徽省自然科学基金项目(1408085MKL38) 安徽省特色创新建设资金项目(15czs08032) 安徽省教委自然科学基金项目(KJ2016A216)~~
【分类号】：O657.72;TB484

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