基于微球透镜的超分辨薄膜成像的特性研究

发布时间：2018-06-03 21:39

本文选题：微球透镜 + 超分辨成像　；参考：《南京师范大学》2017年硕士论文

【摘要】：众所周知,光学显微技术在科学研究中占据举足轻重地位。但是受到传统光学衍射极限影响,传统光学显微镜的分辨率大约只有照明波长一半。近年来,借助介质微球突破衍射极限成为实现超分辨成像一种简便有效的方法。本文提出了一种简单有效的超分辨薄膜制备方法,并对基于介质微球的超分辨薄膜成像特性进行了研究。本论文的主要内容包括:(1)本文提出了一种新的基于介质微球超分辨薄膜制备方法,通过使用普通透明胶背面作为薄膜衬底,制备了厚度为几个微米的超分辨薄膜。(2)研究了浸没在不同厚度聚二甲基硅氧烷(Polydimethylsiloxane,PDMS)薄膜中的钛酸钡(Barium Titanate Glass,BTG)微球成像特性。实验结果显示,随着PDMS薄膜厚度增加,成像放大倍数变化不大,但视场变化显著。当PDMS薄膜厚度在5-10 μm时,微球视场达到最大。为分析视场大小变化的原因,我用Computer Simulation Technology (CST)软件对不同厚度PDMS薄膜进行模拟仿真。此外,我还制备了 7.4μm厚度的PDMS薄膜,分别研究了 2, 3, 4和6个微球紧密排列时的超分辨成像特性。(3)在微球透镜与样品表面增加一层介质层,研究了不同厚度的SU-8光刻胶对微球成像特性的影响。实验发现:随着SU-8厚度从0增大到270 nm,成像视场大小从10.8 μm增加到了 13.2μm。当SU-8厚度在160nm左右时候,成像视场大小与对比度最优。此外,对于厚度为160 nm的不同材料介质层,发现SU-8最能提高视场大小,而二氧化钛(TiO2)介质层却削弱了视场大小。我认为耦合进介质层的光信息含量影响了成像质量,并通过光波干涉相消对这一现象进行了分析。
[Abstract]:As we all know, optical microscopy plays an important role in scientific research. However, the resolution of traditional optical microscopy is about half of the lighting wavelength. In recent years, it is a simple and effective method to achieve superresolution imaging with the help of medium microspheres to break through the diffraction limit. A simple and effective super-resolution film preparation method and the characteristics of super-resolution film imaging based on dielectric microspheres are studied. The main contents of this paper include: (1) a new preparation method based on dielectric microsphere super-resolution film is proposed. The thickness is prepared by using the back of the ordinary transparent adhesive as the film substrate. (2) the imaging characteristics of barium titanate (Barium Titanate Glass, BTG) microspheres immersed in Polydimethylsiloxane (PDMS) thin films with different thickness are studied. The experimental results show that with the increase of the thickness of the PDMS film, the large multiplier of the imaging has not changed much, but the field of view changes remarkably. When the thickness of the PDMS film is in the thickness of the PDMS film, At 5-10 m, the field of view reaches the maximum. In order to analyze the cause of the change of field of view, I use Computer Simulation Technology (CST) software to simulate the different thickness of PDMS film. In addition, I also prepared the PDMS thin film with the thickness of 7.4 mu m, and studied the super-resolution imaging characteristics of 2, 3, 4 and 6 microspheres respectively. (3) The microsphere lens and the sample surface add a layer of media to study the effect of SU-8 photoresist on the imaging characteristics of the microspheres with different thickness. The experimental results show that as the thickness of SU-8 increases from 0 to 270 nm, the image field size is increased from 10.8 to 13.2 to M., when the thickness of SU-8 is around 160nm, the size and contrast of the imaging field is optimal. In different material layers with a thickness of 160 nm, it is found that SU-8 can improve the field of view most, while the titanium dioxide (TiO2) layer weakens the size of the field of view. I think the optical information content of the coupled dielectric layer affects the imaging quality, and the phenomenon is analyzed by the interference of light wave interference.
【学位授予单位】：南京师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O484

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