层层自组装法制备仿生刚性生物相容性聚电解质/碳酸钙薄膜
发布时间：2021-12-09 07:01
生物医学工程的理想生物材料应模仿天然组织的内在特性,特别是要有一定的机械强度,使得细胞能够有效附着。此外,优异的细胞亲和力和组织粘附性使得植入后能够与周围组织整合。有机/无机生物启发的纳米复合材料具有突出的机械和生物化学性质,已经进行了广泛的研究,尽管将生物相容性等功能性与机械性能相结合是一项挑战,具有多功能性。受大自然的启发,我们通过逐层方法开发了使用聚丙烯酸（PAH）,聚（酰氨基胺）（PAMAM）和纳米碳酸钙（纳米碳酸钙）的聚电解质的纳米复合材料,在生物医学领域具有潜在的应用。使用原子力显微镜（AFM）,扫描电子显微镜（SEM）,透射电子显微镜（TEM）,X射线衍射（XRD）,傅里叶变换红外光谱（FTIR）和拉曼光谱表征制备的混合纳米复合膜。此外,评估了复合膜的机械和功能特性。在矿化之后,合成的复合膜显示出显着增强的机械性能,与平均杨氏模量为5.2GPa的非矿化复合膜相比,平均杨氏模量为10.6GPa。此外,与对照样品相比,矿化复合膜显示出改善的压电性质。使用LIVE/DEAD测定法在人肺成纤维细胞（hLFC）上测试复合材料的细胞相容性评估,结果表明对细胞的毒性可忽略不计。细胞附着...
【文章来源】：中国地质大学(北京)北京市 211工程院校 教育部直属院校
【文章页数】：69 页
【学位级别】：硕士
【文章目录】：
List of Abbreviations
摘要
Abstract
Chapter 1 Introduction
1.1 Research background and significance of the study
1.1.1 LbL Self Assembly Technology
1.1.2 Dendrimers
1.1.3 Calcium carbonate
1.2 Research history and present situation
1.2.1 PAA/ CaCO_3 films
1.2.2 Protein/ CaCO_3 films
1.2.3 Cellulose derivatives/ CaCO_3 films
1.2.4 Other organic/ CaCO_3 films
1.3 Purpose and significant of research
1.3.1 Research purpose
1.3.2 Significant of research
1.4 Research content
Chapter 2 Fabrication of LbL polyelectrolyte (PAH/PAMAM)_(7.5) multilayer film
2.1 Introduction
2.2 Preparation of LbL polyelectrolyte (PAH/PAMAM)_(7.5) multilayer film
2.2.1 Experimental raw materials
2.2.2 Experimental instruments and equipment
2.2.3 Experimental procedure and sample preparation
2.2.3.1 Preparation of the Piezoelectric Composite Film PVDF-HFP
2.2.3.2 Fabrication of the Crosslinked PAH/PAMAM Multilayers on the Piezoelectric Film/Quartz/ITOSubstrates
2.3 Results and Discussions
2.3.1 Film Preparation
2.4 Summary of this chapter
Chapter 3 Fabrication and characterization of CaCO_3 on (PAH/PAMAM)_(7.5)- multilayer film
3.1 Introduction
3.2 Preparations and characterization of CaCO_3 on (PAH/PAMAM)_(7.5) multilayer film
3.2.1 Experimental raw materials
3.2.2 Experimental instruments and equipment
3.2.3 Experimental procedure and sample preparation
3.2.3.1 Mineralization of CaCO_3 nano particles in the LbL PAH/PAMAM matrices
3.2.3.2 Film thickness, Morphological and compositional characterizations
3.3 Results and Discussions
3.3.1 Film thickness, Morphological characterizations
3.3.2 Composition analysis
3.4 Summary of this chapter
Chapter 4 Property characterizations of (PAH/PAMAM)_(7.5)-CaCO_3 multilayer film
4.1 Introduction
4.2 Property characterization of CaCO_3 on (PAH/PAMAM)_(7.5) multilayer film
4.2.1 Experimental raw materials
4.2.2 Experimental instruments and equipment
4.2.3 Experimental procedure and sample preparation
4.2.3.1 Mechanical properties of (PAH/PAMAM)_(7.5) before and after mineralization
4.2.3.2 Electrical properties of (PAH/PAMAM)_(7.5) before and after mineralization
4.3 Results and Discussions
4.3.1 Young's moduli
4.3.2 Piezoelectric Properties
4.3.3 Electrochemical impedance spectroscopy (EIS)
4.4 Summary of this chapter
Chapter 5 Applications of (PAH/PAMAM)_(7.5)-CaCO_3 multilayer film
5.1 Introduction
5.2 Test Instruments
5.2.1 Experimental raw materials
5.2.2 Experimental instruments and equipment
5.2.3 Experimental procedure and sample preparation
5.2.3.1 The Loading and Release of Bio macro molecules
5.2.3.2 Cell culture and Cytocompatibility test
5.3 Results and Discussions
5.3.1 Molecular load and release of Methylene blue (MB) into the matrix before and after mineralization
5.3.2 Molecular load and release of Gentamycin Sulfate (GS) into the matrix after mineralization
5.3.3 Cell culture and Cytocompatibility
5.4 Summary of this chapter
Chapter 6 Conclusions
6.1 Overall conclusions
6.2 The main innovation of this paper
6.3 Future works
References
Acknowledgements
本文编号：3530164
【文章来源】：中国地质大学(北京)北京市 211工程院校 教育部直属院校
【文章页数】：69 页
【学位级别】：硕士
【文章目录】：
List of Abbreviations
摘要
Abstract
Chapter 1 Introduction
1.1 Research background and significance of the study
1.1.1 LbL Self Assembly Technology
1.1.2 Dendrimers
1.1.3 Calcium carbonate
1.2 Research history and present situation
1.2.1 PAA/ CaCO_3 films
1.2.2 Protein/ CaCO_3 films
1.2.3 Cellulose derivatives/ CaCO_3 films
1.2.4 Other organic/ CaCO_3 films
1.3 Purpose and significant of research
1.3.1 Research purpose
1.3.2 Significant of research
1.4 Research content
Chapter 2 Fabrication of LbL polyelectrolyte (PAH/PAMAM)_(7.5) multilayer film
2.1 Introduction
2.2 Preparation of LbL polyelectrolyte (PAH/PAMAM)_(7.5) multilayer film
2.2.1 Experimental raw materials
2.2.2 Experimental instruments and equipment
2.2.3 Experimental procedure and sample preparation
2.2.3.1 Preparation of the Piezoelectric Composite Film PVDF-HFP
2.2.3.2 Fabrication of the Crosslinked PAH/PAMAM Multilayers on the Piezoelectric Film/Quartz/ITOSubstrates
2.3 Results and Discussions
2.3.1 Film Preparation
2.4 Summary of this chapter
Chapter 3 Fabrication and characterization of CaCO_3 on (PAH/PAMAM)_(7.5)- multilayer film
3.1 Introduction
3.2 Preparations and characterization of CaCO_3 on (PAH/PAMAM)_(7.5) multilayer film
3.2.1 Experimental raw materials
3.2.2 Experimental instruments and equipment
3.2.3 Experimental procedure and sample preparation
3.2.3.1 Mineralization of CaCO_3 nano particles in the LbL PAH/PAMAM matrices
3.2.3.2 Film thickness, Morphological and compositional characterizations
3.3 Results and Discussions
3.3.1 Film thickness, Morphological characterizations
3.3.2 Composition analysis
3.4 Summary of this chapter
Chapter 4 Property characterizations of (PAH/PAMAM)_(7.5)-CaCO_3 multilayer film
4.1 Introduction
4.2 Property characterization of CaCO_3 on (PAH/PAMAM)_(7.5) multilayer film
4.2.1 Experimental raw materials
4.2.2 Experimental instruments and equipment
4.2.3 Experimental procedure and sample preparation
4.2.3.1 Mechanical properties of (PAH/PAMAM)_(7.5) before and after mineralization
4.2.3.2 Electrical properties of (PAH/PAMAM)_(7.5) before and after mineralization
4.3 Results and Discussions
4.3.1 Young's moduli
4.3.2 Piezoelectric Properties
4.3.3 Electrochemical impedance spectroscopy (EIS)
4.4 Summary of this chapter
Chapter 5 Applications of (PAH/PAMAM)_(7.5)-CaCO_3 multilayer film
5.1 Introduction
5.2 Test Instruments
5.2.1 Experimental raw materials
5.2.2 Experimental instruments and equipment
5.2.3 Experimental procedure and sample preparation
5.2.3.1 The Loading and Release of Bio macro molecules
5.2.3.2 Cell culture and Cytocompatibility test
5.3 Results and Discussions
5.3.1 Molecular load and release of Methylene blue (MB) into the matrix before and after mineralization
5.3.2 Molecular load and release of Gentamycin Sulfate (GS) into the matrix after mineralization
5.3.3 Cell culture and Cytocompatibility
5.4 Summary of this chapter
Chapter 6 Conclusions
6.1 Overall conclusions
6.2 The main innovation of this paper
6.3 Future works
References
Acknowledgements
本文编号：3530164
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