两种血细胞分离机采集异体外周血造血干细胞效果的比较

发布时间：2018-02-03 01:01

本文关键词： 血细胞分离机造血干细胞异体外周血　出处：《中国输血杂志》2016年03期 　论文类型：期刊论文

【摘要】：目的探讨2种血细胞分离机采集异体外周血造血干细胞的效果。方法对本院2013-2015年异体造血干细胞移植的供者50名进行研究,其中COM.TEC血细胞分离机采集25例,Amicus血细胞分离机采集25例。比较2组采集前后供者的Hct、Plt;产品中Plt、RBC、Hct、以及MNC细胞数、CD34+细胞数、细胞浓度。结果用Amicus和COM.TEC采集的造血干细胞供者的血小板损失率分别为17.67%和48.83%,提示用Amicus采集对供者的血小板损失较少(P0.05);用Amicus和COM.TEC采集的终产品中血小板和RBC的混入量分别为(649±527)vs(4064±1 312)和(0.67±0.26)vs(0.95±0.52),提示Amicus采集的终产品中血小板和RBC的混入量较低(P0.05);用Amicus和COM.TEC采集的CD34+细胞数分别为(3.53±1.97)和(4.67±2.28),提示2种机型采集效果相当(P0.05);用Amicus和COM.TEC采集的细胞浓度和MNC数分别为(2.63±0.80)vs(3.82±0.79)和(3.23±1.19)vs(3.85±0.81),提示COM.TEC采集的细胞浓度和MNC数高于Amicus(P0.05)。结论 2种机型都能为临床采集有效的CD34+细胞,但与COM.TEC相比,Amicus在采集过程中能有效减少血小板的丢失,特别适用于Plt低的供者。
[Abstract]:Objective to investigate the effect of two kinds of blood cell separator on collecting allogeneic peripheral blood hematopoietic stem cells. Methods 50 donors of allogeneic hematopoietic stem cell transplantation in our hospital from 2013 to 2015 were studied. Among them, 25 cases were collected by COM.TEC blood cell separator and 25 cases were collected by Amicus blood cell separator. The number of MNC cells and the number of CD34 cells were also found in the product. Results the platelet loss rates of hematopoietic stem cell donors collected by Amicus and COM.TEC were 17.67% and 48.83% respectively. It is suggested that the platelet loss of donors collected by Amicus is less than that of P0.05. The amount of platelet and RBC mixed in the final product collected by Amicus and COM.TEC were 649 卤527VSD 4064 卤1312) and (. 0.67 卤0.26 vs 0.95 卤0.52). The results suggested that the amount of platelet and RBC mixed in the final product collected by Amicus was lower than that of RBC. The number of CD34 cells collected by Amicus and COM.TEC was 3.53 卤1.97 and 4.67 卤2.28, respectively. It is suggested that the two kinds of models have the same collection effect as that of P0.05; The cell concentrations and MNC numbers collected by Amicus and COM.TEC were 2.63 卤0.80 vs 3.82 卤0.79) and 3.23 卤1.19 vs (P < 0.05). 3.85 卤0.81). The results suggest that the cell concentration and the number of MNC collected by COM.TEC are higher than those of Amicus P0.050.Conclusion both models can be used to collect effective CD34 cells in clinic. Compared with COM.TEC, Amicus can effectively reduce platelet loss, especially for low Plt donors.
【作者单位】：中国人民解放军第307医院输血科;
【分类号】：R457.1
【正文快照】： 外周血造血干细胞移植(peripheral blood stem cell trans-plantation.PBSCT)具有移植后造血功能与免疫功能恢复快,造血干细胞采集时不需麻醉,避免创伤性手术和大失血等优点[1],目前已广泛应用于血液系统恶性肿瘤、自身免疫性疾病和某些实体肿瘤的治疗,随之出现了多种不同型号

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