基于量子点荧光探针的高灵敏蛋白质检测方法

发布时间：2018-03-09 07:56

本文选题：量子点　切入点：免疫荧光分析　出处：《华东师范大学学报(自然科学版)》2016年02期 　论文类型：期刊论文

【摘要】：通过制备量子点荧光检测探针,构建了一种基于量子点探针和免疫磁珠的蛋白质检测方法,实现了对蛋白肿瘤标志物癌胚抗原(carcinoembryonic antigen,CEA)的高灵敏定量检测.在该检测体系中,若存在有靶标蛋白,其与量子点检测探针以及捕获探针之间会发生免疫反应形成三明治结构,利用磁力分离器对免疫复合物进行富集后,通过检测富集在磁珠表面的量子点荧光信号,可实现对靶标蛋白的定量.该方法的检测灵敏度为38 pg/mL,线性范围为0.39~50ng/mL,临床质控样本检验结果表明,该方法准确度高,可重复性好,可应用于临床样本检测.该量子点探针检测体系具有灵敏度高、特异性好、样品消耗量低等优点,在疾病早期诊断方面具有广阔的应用前景.
[Abstract]:A method of protein detection based on quantum dot probe and immunomagnetic beads was constructed by preparing quantum dot fluorescence detection probe. A highly sensitive and quantitative detection method for carcinoembryonic antigen (CEA), a protein tumor marker, was developed. If there is a target protein, the immune reaction between the target protein and the quantum dot detection probe and the capture probe will form a sandwich structure, and the immune complex can be enriched by magnetic separator. The quantification of target protein can be achieved by detecting the fluorescence signals of quantum dots enriched on the magnetic bead surface. The sensitivity of the method is 38 PG / mL and the linear range is 0.39 ~ 50 ng / mL. The results of clinical quality control samples show that the method is accurate and reproducible. The quantum dot probe detection system has the advantages of high sensitivity, good specificity and low sample consumption, so it has a broad application prospect in the early diagnosis of disease.
【作者单位】：华东师范大学生命科学学院;中国科学院上海微系统与信息技术研究所传感技术联合国家重点实验室;
【基金】：国家重点基础发展计划(2012CB933303) 国家自然科学基金(81101645;61271162;81472751;21405167) 上海市科委项目(12nm0503702;11JC1414400;BRC2012002) 东南大学生物电子学国家重点实验室开放研究项目
【分类号】：R446.1;O657.3

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