一株人感染H9N2禽流感病毒高通量测序及序列分析

发布时间：2018-03-16 11:52

  本文选题：流感病毒A型　切入点：HN亚型　出处：《中华疾病控制杂志》2016年11期 　论文类型：期刊论文

【摘要】：目的对一株人感染H9N2禽流感病毒进行高通量测序(next-generation sequencing,NGS)分析,探讨其在人群流行的可能性。方法应用高通量测序技术进行全基因组序列测定(whole genome sequencing,WGS)。对8个基因进行相似性检索,构建系统进化树并分析其关键位点的分子特征。结果 8个基因与Gen Bank基因库相似度最高序列的来源不完全一致,系统进化树显示HA基因属于欧亚系I群,M基因位于G1-like分支,PB2位于G9-like分支,NA位于一独立分支,PBl,PA,NP,NS均位于SH/F/98-like分支。HA基因裂解位点为PSRSSR/GLF,226位受体结合位点为L。除M2基因S31N突变之外,NA基因茎63~65位缺失,PA和PB2基因未发生L336M和Q591R,E627K等可以增强病毒对哺乳动物适应性的突变,但发现可以增强病毒毒力的突变如M1基因N30D和T215A,PB2基因L89V,NSl基因P42S。除M2基因S31N突变外,NA基因和M2基因的药物结合位点未发生E119G,R152K,H274Y,R292K和L26F,V27A,A30T,G34E等耐药性突变。HA和NA糖基化位点预测结果都有8个糖基化位点,其中分别有7个和5个可靠程度较高。结论该H9N2禽流感病毒的大部分关键性位点较保守,只有少部分位点发生一定程度进化与变异,在人群引起流行的可能性不大,但需加强分子方面动态监测。
[Abstract]:Objective to analyze a human H9N2 avian influenza virus (H9N2 avian influenza virus) by high-throughput sequencing next-generation sequencing (NGS) and to explore the possibility of its prevalence in human population. Methods High-throughput sequencing technique was used to determine the whole genome sequencing sequence of the whole genome of H9N2 Avian Influenza virus (H9N2) and to search the similarity of eight genes. The phylogenetic tree was constructed and the molecular characteristics of its key loci were analyzed. The results showed that the source of the highest similarity sequence between the eight genes and the Gen Bank gene pool was not identical. Phylogenetic tree showed that HA gene belongs to Eurasian line I group I, M gene is located in G1-like branch PB2, located in G9-like branch na, located in a separate branch of PBlBlPANPNs, located at SH/F/98-like branch. HA gene cleavage site is PSRSSR-GLF226 receptor binding site, except M2 gene S31N process site. No mutations in L336M and Q591RRNE627K genes could enhance the adaptability of the virus to mammals. However, mutants such as M1 gene N30D and T215AfN PB2 gene L89VGN NSl gene P42S were found to enhance virulence of the virus. Except for M2 gene S31N mutation, there were no drug binding sites of NNA gene and M2 gene. There were no drug binding sites such as E119GfN R152KH274YP292K and L26FV27A3A30TG34E mutation. Ha and na glycosylation sites were not found. All of them had eight glycosylation sites. Conclusion most of the key sites of H9N2 avian influenza virus are conservative, and only a few of them have evolved and mutated to a certain extent. But it is necessary to strengthen molecular dynamic monitoring.
【作者单位】： 中山市疾病预防控制中心检验科;
【基金】：广东省中山市科技计划项目(2015B1129)
【分类号】：R511.7;R440
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本文编号：1619795