胰高血糖素样肽-2对全肠外营养小鼠肠黏膜和肝功能的保护作用

发布时间：2018-03-23 21:40

本文选题：胰高血糖素样肽-　切入点：全肠外营养　出处：《肠外与肠内营养》2016年04期

【摘要】：目的:探讨胰高血糖素样肽-2(GLP-2)对全肠外营养(TPN)小鼠肠黏膜结构与肝功能的影响及相关机制。方法:将30只小鼠随机分为三组,即自由进食组(Chow组)、TPN组和TPN+GLP-2组。小鼠经颈外静脉置管2 d后,TPN组接受TPN液,TPN+GLP-2组除输注等量上述营养液外,还经颈外静脉注射60μg/d的GLP-2。第7天处死小鼠,取眼球静脉血检测肝功能,留取十二指肠、回肠末端和肝组织,检测相关蛋白和mRNA表达,并观察肠黏膜和肝组织的形态学改变。结果:7 d后,TPN组小鼠体重明显下降,小肠绒毛高度与隐窝深度均劣于TPN+GLP-2组和Chow组(P0.05)。TPN组小鼠肠黏膜损伤较严重(P0.05),其血清肝功能指标水平较TPN+GLP-2组和Chow组高(P0.05),而清蛋白水平较其他两组低(P0.05)。病理显示TPN组小鼠肝细胞变性坏死,小肠微绒毛稀少,排列不齐,出现倒伏、脱落,上皮细胞线粒体破坏严重,内有大量空泡。TPN+GLP-2组肠PCNA蛋白表达较TPN组多(P0.05),而cleaved caspase-3表达较少(P0.05)。TPN+GLP-2组肠LGR-5 mRNA表达较TPN组多(P0.05),而肝GRP94 mRNA表达较低(P0.05)。结论:GLP-2可减轻TPN治疗引起的小鼠肠黏膜损伤,对维护肝功能有一定的保护作用。
[Abstract]:Objective: to investigate the effects of glucagon like peptide-2 (GLP-2) on intestinal mucosal structure and liver function in TPN mice. Methods: thirty mice were randomly divided into three groups. The mice received TPN solution TPN GLP-2 for 2 days. In addition to the same amount of the above nutrient solution, the mice were given 60 渭 g / d GLP-2.The mice were killed on the 7th day. The blood of eyeball vein was taken to detect liver function, duodenum, ileum terminal and liver tissue, expression of related protein and mRNA were detected, and morphological changes of intestinal mucosa and liver tissue were observed. Results the weight of mice in TPN group decreased significantly after 7 days. The villus height and crypt depth of small intestine were lower than those of TPN GLP-2 group and Chow group (P0.05. TPN group). The serum liver function index level was higher than that of TPN GLP-2 group and Chow group, while the level of albumin was lower than that of other two groups. The pathological findings showed that TPN was higher than that in TPN GLP-2 group and Chow group. Group B mice hepatocyte degeneration and necrosis, Small intestinal microvilli are rare, irregular, lodging, shedding, and the mitochondria of epithelial cells are destroyed seriously. There were a large number of vacuoles. TPN GLP-2 group expressed more PCNA protein than TPN group, while cleaved caspase-3 expression was less in the group of cleaved GLP-2 than that in the group of TPN. The expression of LGR-5 mRNA was lower in group A than that in group TPN, but the expression of GRP94 mRNA in liver was lower than that in group A (P 0.05). Conclusion the expression of cleaved caspase-3 in the intestine of mice treated with TPN can be alleviated. It can protect liver function to some extent.
【作者单位】：南方医科大学;南京军区南京总医院解放军普通外科研究所;
【基金】：国家自然科学基金资助项目(81470797)
【分类号】：R459.3

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