在慢性睡眠剥夺中骨桥蛋白通过NF-kappaB通路对颞下颌关节的影响

发布时间：2018-05-05 10:23

本文选题：慢性睡眠剥夺 + 颞下颌关节　；参考：《山东大学学报(医学版)》2016年09期

【摘要】：目的探讨骨桥蛋白(OPN)在慢性睡眠剥夺(CSD)引起大鼠颞下颌关节(TMJ)结构变化中的作用。方法采用改良多平台法(MMPM)建立大鼠的慢性睡眠剥夺模型。180只雄性Wistar大鼠随机分为2组:对照组(CON组)、慢性睡眠剥夺组(CSD组),每组90只。每组根据实验时间不同分为3个亚组:4、6、8周。通过HE染色观察大鼠TMJ的组织学结构变化;通过免疫组织化学染色检测OPN蛋白表达变化;通过免疫组织化学染色和免疫荧光检测NF-κB蛋白表达变化。结果 CSD组的大鼠髁突均出现不同程度的病理变化,随着睡眠剥夺时间延长,软骨损伤更加严重。与CON组相比,CSD组OPN、NF-κB的蛋白表达水平明显升高(P0.05),随睡眠剥夺时间延长,OPN、NF-κB表达均呈增加趋势。结论慢性睡眠剥夺能够激活大鼠颞下颌关节髁突软骨中软骨细胞及各种炎症细胞生成骨桥蛋白增多,OPN通过激活NF-κB通路,进而引起大鼠颞下颌关节发生病理性改变。
[Abstract]:Objective to investigate the role of osteopontin (OPN) in the structural changes of TMJs induced by chronic sleep deprivation (CSD) in rat temporomandibular joint (TMJ). Methods one hundred and eighty male Wistar rats were randomly divided into two groups: control group (n = 90), chronic sleep deprivation group (n = 90) and chronic sleep deprivation group (n = 90). Each group was divided into 3 subgroups according to the time of the experiment. The histological and structural changes of rat TMJ were observed by HE staining; the expression of OPN protein was detected by immunohistochemical staining; the expression of NF- 魏 B protein was detected by immunohistochemical staining and immunofluorescence. Results the condylar process in CSD group showed pathological changes in varying degrees. With the prolongation of sleep deprivation, cartilage injury became more serious. Compared with CON group, the expression of NF- 魏 B in CON group was significantly higher than that in CON group, and the expression of NF- 魏 B increased with the prolongation of sleep deprivation. Conclusion chronic sleep deprivation can activate chondrocytes and inflammatory cells of temporomandibular joint (TMJ) to produce osteopontin (OPN). OPN can activate NF- 魏 B pathway and induce pathological changes of temporomandibular joint in rats.
【作者单位】：锦州医科大学实习基地济南军区总医院口腔科;山东大学口腔医学院山东省口腔组织再生重点实验室;中国人民解放军济南军区总医院口腔科;
【基金】：国家自然科学基金(81400573)
【分类号】：R782.6;R740

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