快速振荡法高效提取小鼠腹腔巨噬细胞研究

发布时间：2018-05-27 15:22

本文选题：巨噬细胞 + 小鼠　；参考：《中国全科医学》2016年03期

【摘要】：目的通过与传统的腹腔巨噬细胞提取方法——轻揉法进行比较,建立一种高效提取小鼠腹腔巨噬细胞体外分离培养的方法——快速振荡法。方法 2014年11月选取雄性健康SPF级C57b1/6小鼠20只,采用随机数字表法分为轻揉组和振荡组,各10只。分别采用传统的腹部轻揉法和快速振荡法提取小鼠腹腔巨噬细胞。流式细胞仪检测巨噬细胞凋亡率,计算细胞总数及巨噬细胞总数。结果两组小鼠体质量和腹腔灌洗液回收率比较,差异无统计学意义(P0.05)。轻揉组巨噬细胞凋亡率为(5.44±0.08)%,振荡组巨噬细胞凋亡率为(5.60±0.12)%,差异无统计学意义(t=1.09,P=0.29)。振荡组细胞总数[(5.69±0.23)×10~6与(3.82±0.12)×10~6]和巨噬细胞总数[(2.64±0.08)×10~6与(1.66±0.07)×10~6]均多于轻揉组(P0.05)。结论快速振荡法提取小鼠腹腔巨噬细胞,巨噬细胞凋亡率无增加,细胞总数及巨噬细胞总数明显增加,是一种更加高效的提取腹腔巨噬细胞的方法。
[Abstract]:Objective to establish a rapid oscillating method for the isolation and culture of mouse peritoneal macrophages in vitro by comparing with the traditional method of peritoneal macrophage extraction. Methods Twenty healthy male SPF C57b1/6 mice were selected in November 2014. They were randomly divided into two groups: the light kneading group and the oscillating group with 10 mice in each group. Mouse peritoneal macrophages were extracted by traditional abdominal rubbing method and rapid oscillation method respectively. The apoptosis rate of macrophages was measured by flow cytometry, and the total number of macrophages were calculated. Results there was no significant difference in body weight and recovery of peritoneal lavage fluid between the two groups (P 0.05). The apoptosis rate of macrophages was 5.44 卤0.08 in light rubbing group and 5.60 卤0.12 in oscillating group. The difference was not statistically significant. The total number of cells [5.69 卤0.23) 脳 10 ~ (6) and 3.82 卤0.12) 脳 10 ~ (6) and total number of macrophages [2.64 卤0.08) 脳 10 ~ (6) and 1.66 卤0.07 脳 10 ~ (6) 脳 10 ~ (6) in oscillating group were more than those in light rubbing group (P 0.05). Conclusion Rapid oscillatory extraction of murine peritoneal macrophages is a more efficient method for the extraction of peritoneal macrophages, but the apoptosis rate of macrophages is not increased, but the total number of macrophages and the total number of macrophages are obviously increased.
【作者单位】：华中科技大学同济医学院附属协和医院麻醉科;
【基金】：国家自然科学基金青年基金资助项目(81201454)
【分类号】：R446.8

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