应急快速ABO及RhD固相法血型定型技术的评估研究

发布时间：2018-07-24 19:50

【摘要】：目的现有血型鉴定、交叉配血等技术难以满足应急输血的需求,应急快速ABO及RhD固相法血型定型技术克服了现有技术在突发事件条件下使用的缺陷。文中旨在探讨应用应急快速ABO及RhD固相法血型定型技术进行应急血型检测的可行性。方法选择2016年2月至2016年7月南京医科大学附属江宁医院输血科常规住院拟输血、申请血型鉴定的患者1258例。所有患者抽取静脉血3m L,EDTA抗凝。血型鉴定于标本送达后4h内完成,在不同的温度条件下(4、22和37℃),采用应急快速ABO及RhD固相法血型定型试剂检测患者的全血样本,以微柱凝胶卡法结果为对照,评估应急快速ABO及RhD固相法血型定型技术的准确性和特异性。结果与微柱凝胶卡法比较,应急快速ABO及RhD固相法血型定型试剂对所有全血样本定型符合率均为100%。应急快速ABO及RhD固相法各温度下温度适应性检测结果的符合率均为100%。结论应急快速ABO及RhD固相法血型定型技术具有良好的准确性,适合应急血型初筛。
[Abstract]:Objective the existing blood type identification and cross matching techniques are difficult to meet the needs of emergency blood transfusion. The emergency rapid ABO and RhD solid phase blood typing technology overcome the defects of the existing technology in the emergency condition. The purpose of this paper is to explore the feasibility of emergency blood group detection by using emergency rapid ABO and RhD solid-phase blood typing technique. Methods from February 2016 to July 2016, 1258 patients applying for blood type identification were selected from the Department of Blood Transfusion Department of Jiangning Hospital affiliated to Nanjing Medical University. All patients were drawn from venous blood 3m Ln EDTA anticoagulant. Blood group identification was completed within 4 hours after sample delivery. At different temperatures (40.22 and 37 鈩，

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