人副流感病毒的快速分离培养与鉴定
发布时间：2018-08-06 21:10
【摘要】：目的副流感病毒是婴幼儿下呼吸道感染的重要病原体,为快速准确诊断副流感病毒感染,文中探讨一种从鼻拭子快速分离培养及鉴定副流感病毒的方法。方法采集0~5岁诊断为急性呼吸道感染的患儿鼻拭子标本,以3000r/min离心1 h接种至96孔板预制LLC-MK2细胞以及按照传统培养法进行接种,最后补充含4μg/m L TPCK胰酶的病毒维持培养基。每天观察细胞病变,培养第2、5、8天分别作血吸附、血凝试验,试验阳性时作免疫荧光鉴定。结果从83份鼻拭子标本中分离鉴定6株副流感病毒,阳性率7.2%。离心培养法与传统培养法在标本培养2 d后其分离率分别为7.2%和0,差异有统计学意义(P0.05)。阳性标本感染的细胞镜下可见局部变长、融合、破碎的细胞病变,血凝试验4℃凝集而室温不凝集,血吸附试验4℃阳性而室温阴性,免疫荧光可见细胞内特异性苹果绿荧光。结论快速分离培养方法可最快在2 d内从鼻拭子标本中获得有毒力和生物活性的副流感病毒并进行鉴定。
[Abstract]:Objective Parainfluenza virus is an important pathogen of infantile lower respiratory tract infection. In order to diagnose parainfluenza virus infection quickly and accurately, a method of rapid isolation, culture and identification of parainfluenza virus from nasal swab is discussed. Methods nasal swabs were collected from children aged 0 to 5 years with acute respiratory tract infection. Prefabricated LLC-MK2 cells were inoculated with 3000r/min centrifugation for 1 h to 96 well plates and inoculated according to the traditional culture method. Finally, the virus maintenance medium containing 4 渭 g / mL TPCK trypsin was added. The cytopathic changes were observed every day. Blood adsorption, hemagglutination test and immunofluorescence assay were performed on the 5th day of culture. Results 6 strains of parainfluenza virus were isolated from 83 nasal swabs and the positive rate was 7.2%. The isolation rates of centrifuge culture method and traditional culture method were 7.2% and 0 respectively after 2 days of culture. The difference was statistically significant (P0.05). The infected cells of positive specimens showed local lengthening, fusion, broken cytopathic changes, hemagglutination test 4 鈩，
本文编号：2168998
[Abstract]:Objective Parainfluenza virus is an important pathogen of infantile lower respiratory tract infection. In order to diagnose parainfluenza virus infection quickly and accurately, a method of rapid isolation, culture and identification of parainfluenza virus from nasal swab is discussed. Methods nasal swabs were collected from children aged 0 to 5 years with acute respiratory tract infection. Prefabricated LLC-MK2 cells were inoculated with 3000r/min centrifugation for 1 h to 96 well plates and inoculated according to the traditional culture method. Finally, the virus maintenance medium containing 4 渭 g / mL TPCK trypsin was added. The cytopathic changes were observed every day. Blood adsorption, hemagglutination test and immunofluorescence assay were performed on the 5th day of culture. Results 6 strains of parainfluenza virus were isolated from 83 nasal swabs and the positive rate was 7.2%. The isolation rates of centrifuge culture method and traditional culture method were 7.2% and 0 respectively after 2 days of culture. The difference was statistically significant (P0.05). The infected cells of positive specimens showed local lengthening, fusion, broken cytopathic changes, hemagglutination test 4 鈩，
本文编号：2168998
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