耐甲氧西林金黄色葡萄球菌pvl及tst基因分布与感染类型研究
发布时间：2018-09-17 10:29
【摘要】：目的了解耐甲氧西林金黄色葡萄球菌(MRSA)临床菌株携带杀白细胞毒素(PVL)编码基因pvl和中毒休克综合征毒素-1(TSST-1)编码基因tst情况、流行特征及感染类型,为该类感染的临床治疗提供依据。方法收集2012年1-12月临床分离的MRSA 96株,采用PCR检测96株MRSA中pvl和tst基因,采用全自动微生物分析仪检测11种临床常用抗菌药物的敏感性,采用多重PCR对携带pvl基因(pvl+)或tst基因(tst+)的MRSA进行mec分型(SCCmec),了解与分析pvl+或tst+MRSA感染类型。结果 96株MRSA中20株检出pvl基因占20.8%,13株检出tst基因占13.5%,未发现同时携带pvl和tst基因的菌株;除左氧氟沙星和莫西沙星外,pvl+/tst+MRSA对其他抗菌药物耐药率与pvl-/tst-菌株相似;20株pvl+MRSA中7株社区获得性感染菌株、13株医院获得性感染菌株,其中17株分离自皮肤和软组织感染患者脓液,13株tst+MRSA均为医院获得感染菌株,其中8株分离自肺部感染患者痰液;20株pvl+MRSA中,SCCmecⅢ型11株、SCCmecⅣa型5株、SCCmecII型2株,2株未能分型;13株tst+MRSA均为SCCmecⅢ型。结论 pvl+MRSA在社区和医院内均有流行,主要引起皮肤和软组织感染;tst+MRSA仅在医院内流行,主要引起肺部感染;pvl+和tst+MRSA均以SCCmecⅢ型为主。
[Abstract]:Objective to investigate the prevalence, prevalence and type of infection in clinical strains of methicillin-resistant Staphylococcus aureus (MRSA) carrying leukocytotoxin (PVL) coding gene (pvl) and toxic shock syndrome toxin 1 (TSST-1) gene tst. To provide the basis for the clinical treatment of this kind of infection. Methods 96 strains of MRSA isolated from January to December 2012 were collected. The genes of pvl and tst in 96 strains of MRSA were detected by PCR, and the sensitivity of 11 kinds of commonly used antimicrobial agents were detected by automatic microbiological analyzer. Mec typing of MRSA carrying pvl gene (pvl) or tst gene (tst) was conducted by multiple PCR and pvl or tst MRSA infection types were analyzed. Results out of 96 MRSA strains, 20 pvl genes were detected in 20.8G and 13 tst genes were detected in 13 strains, and no pvl and tst gene carriers were found. With the exception of levofloxacin and moxifloxacin, PVL / TST MRSA was resistant to other antimicrobial agents, which were similar to those of pvl-/tst- strains, including 7 community-acquired infections (pvl MRSA) and 13 nosocomial infections (Nosocomial). Among them, 17 strains were isolated from patients with skin and soft tissue infection and 13 strains of tst MRSA were nosocomial infected strains. Among the 20 sputum strains isolated from patients with pulmonary infection, 11 strains of SCCmec 鈪，
本文编号：2245562
[Abstract]:Objective to investigate the prevalence, prevalence and type of infection in clinical strains of methicillin-resistant Staphylococcus aureus (MRSA) carrying leukocytotoxin (PVL) coding gene (pvl) and toxic shock syndrome toxin 1 (TSST-1) gene tst. To provide the basis for the clinical treatment of this kind of infection. Methods 96 strains of MRSA isolated from January to December 2012 were collected. The genes of pvl and tst in 96 strains of MRSA were detected by PCR, and the sensitivity of 11 kinds of commonly used antimicrobial agents were detected by automatic microbiological analyzer. Mec typing of MRSA carrying pvl gene (pvl) or tst gene (tst) was conducted by multiple PCR and pvl or tst MRSA infection types were analyzed. Results out of 96 MRSA strains, 20 pvl genes were detected in 20.8G and 13 tst genes were detected in 13 strains, and no pvl and tst gene carriers were found. With the exception of levofloxacin and moxifloxacin, PVL / TST MRSA was resistant to other antimicrobial agents, which were similar to those of pvl-/tst- strains, including 7 community-acquired infections (pvl MRSA) and 13 nosocomial infections (Nosocomial). Among them, 17 strains were isolated from patients with skin and soft tissue infection and 13 strains of tst MRSA were nosocomial infected strains. Among the 20 sputum strains isolated from patients with pulmonary infection, 11 strains of SCCmec 鈪，
本文编号：2245562
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