多重PCR在多重耐药鲍曼不动杆菌耐药基因检测中的应用

发布时间：2018-11-25 22:16

【摘要】：目的建立能够同时检测多重耐药鲍曼不动杆菌(Multidrug-Resistant Acinetobacter Baumannii,MDRAB)多个耐药基因的多重PCR反应体系,了解MDRAB耐药基因分布情况,为临床治疗及感染控制MDRAB提供分子生物学依据。方法针对MDRAB 4种耐药基因blaoxa-23、bla Ade-B、blaint-1及bla ISCR-1分别设计4对引物,设计反应条件在同一PCR反应体系中同时检测这4种耐药基因,并采用建立的多重PCR反应体系检测临床分离MDRAB耐药基因携带情况。结果本研究成功建立了一个可以同时检测4种MDRAB耐药基因的多重PCR反应体系,临床分离68株MDRAB耐药基因检测显示,1株同时携带有4种耐药基因,5株携带有bla Ade-B、blaint-1及bla ISCR-1,62株携带有blaoxa-23和bla Ade-B。结论多重PCR可以快速、高效、低成本检测MDRAB携带的耐药基因,多重PCR检测显示blaoxa-23及bla Ade-B是MDRAB携带的主要耐药基因。
[Abstract]:Objective to establish a multiplex PCR reaction system for simultaneous detection of multidrug resistant genes of Acinetobacter baumannii (Multidrug-Resistant Acinetobacter Baumannii,MDRAB) and to understand the distribution of MDRAB resistance genes in order to provide molecular biological basis for clinical treatment and infection control of MDRAB. Methods four pairs of primers were designed for blaoxa-23,bla Ade-B,blaint-1 and bla ISCR-1 of four resistant genes of MDRAB, and the reaction conditions were designed to detect the four resistant genes simultaneously in the same PCR reaction system. The multiplex PCR reaction system was used to detect the drug resistance genes carried by clinical isolates of MDRAB. Results in this study, a multiplex PCR reaction system was successfully established, which could simultaneously detect 4 MDRAB resistance genes. 68 clinical isolates of MDRAB resistance genes showed that 1 strain carried 4 drug resistance genes and 5 strains carried bla Ade-B,. Blaint-1 and bla ISCR-1,62 strains carry blaoxa-23 and bla Ade-B. Conclusion Multiplex PCR can be used to detect drug resistance genes carried by MDRAB quickly, efficiently and at low cost. Multiplex PCR analysis shows that blaoxa-23 and bla Ade-B are the major drug resistant genes carried by MDRAB.
【作者单位】：南方医科大学基础医学院医学遗传学教研室;南方医科大学第三附属医院检验科;
【分类号】：R440

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