烧伤患者分离金黄色葡萄球菌耐药性与耐药基因研究

发布时间：2019-01-27 08:19

【摘要】：目的了解烧伤病房患者分离金黄色葡萄球菌的耐药性及耐药基因分布,探讨烧伤患者易感染金黄色葡萄球菌的原因。方法收集2013年7-12月医院烧伤病房患者的金黄色葡萄球菌,菌种鉴定采用法国生物梅里埃公司VITEK-2Compact细菌鉴定仪,并进一步作spa基因PCR检测,药敏试验采用法国生物梅里埃公司VITEK-2Compact系统进行,通过PCR方法分析其耐药基因mecA、aac6′/aph2″、aph3′-Ⅲ、ant4′、ermA、ermB、ermC、mphC、msrA、tetM、tetK、qacA、bsa的表达。结果共收集金黄色葡萄球菌20株,其中19株来源于创面、1株来源于留置导管;金黄色葡萄球菌对青霉素G的耐药率为100.0%,对万古霉素、替考拉宁、利福平等耐药率均为0;mecA和bsa基因检测阳性率均为55.0%,aac6′/aph2″、aph 3′-Ⅲ基因检测阳性率分别为50.0%和45.0%。结论烧伤患者分离的20株金黄色葡萄球菌中mecA及bsa基因检出率较高,是烧伤患者易于感染金黄色葡萄球菌的原因,耐药基因检测提示其对季胺类消毒剂消毒皮肤效果可能欠佳。
[Abstract]:Objective to investigate the drug resistance and gene distribution of Staphylococcus aureus isolated from burn ward patients and to explore the causes of Staphylococcus aureus infection in burn patients. Methods Staphylococcus aureus was collected from burn ward patients in our hospital from July to December 2013. The identification of Staphylococcus aureus was carried out by VITEK-2Compact instrument of French Bio-Meridier Company, and the spa gene was detected by PCR. The drug sensitivity test was carried out by VITEK-2Compact system of Bio-Meridier Company of France. The expression of mecA,aac6'/aph2 ", aph3'- 鈪，

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