滞流前后管道生物膜微生物群落径向分布差异

发布时间：2018-01-29 16:12

本文关键词： 供水管道管壁生物膜滞流工况微生物菌落径向分布差异　出处：《浙江大学学报(工学版)》2017年07期 　论文类型：期刊论文

【摘要】：为了探究供水管道管壁生物膜在滞流前、后的细菌数量和微生物群落结构在空间分布上变化的差异性,依托建设于实际供水管网系统中的管网中试平台开展研究.采用电子扫描电镜(SEM)观察管道生物膜的形态差异,采用实时荧光定量PCR计数生物膜中细菌总数,采用高通量测序分析管道不同空间位置上微生物群落种类和含量.研究结果表明,滞流后细菌总数在两种管道上部增加较少,中部和下部增加较大.微生物群落在高密度聚乙烯管道内壁空间位置上分布较均匀,种群结构变化不大,球墨铸铁管中的种群结构分布变化较大.其中腐蚀菌属从空间位置的上部至下部含量依次增加,滞流后管道中部腐蚀菌落含量变化较大,应重点关注管道中部腐蚀菌落对管道腐蚀的影响.
[Abstract]:In order to explore the difference of the bacteria quantity and the microbial community structure in the spatial distribution of the biofilm in the water supply pipeline before and after the stagflation. Based on the pilot platform of water supply network system, the morphological differences of biofilm in pipeline were observed by means of electronic scanning electron microscope (SEM). The total number of bacteria in biofilm was counted by real-time fluorescence quantitative PCR, and the species and content of microbe community in different space position of pipeline were analyzed by high-throughput sequencing. After stagflation, the total number of bacteria increased less in the upper part of the two pipelines, but increased more in the middle and lower parts. The microbe community distributed evenly on the inner wall of HDPE pipeline, and the population structure changed little. The distribution of population structure in ductile iron pipes varies greatly. The content of corrosion bacteria in the middle of ductile iron pipes increases from the upper to the lower of the space position, and the content of corrosion bacteria in the middle of ductile iron pipes changes greatly after stagflation. The influence of corrosion colony on pipeline corrosion should be paid more attention to.
【作者单位】：浙江大学土木工程学系;嘉兴市嘉源给排水有限公司;浙江工业大学建筑工程学院;
【基金】：国家自然科学基金面上资助项目(51378455) 水体污染控制与治理国家科技重大专项资助项目(2012ZX07403-003)
【分类号】：TU991.36
【正文快照】： 在供水系统中管道内壁与饮用水长期接触的表面存在着微生物群落[1]的生长与繁殖所形成的生物膜,管道中约有95%的细菌附着生长在管道内壁生物膜中[2].生物膜是由微生物以及其产生胞外聚合物[3](EPS)和水中的无机物、有机物相互粘和形成的聚合体系.管道内壁生物膜的形成会导致许

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