优秀自由式摔跤运动员的代谢组学研究

发布时间：2017-12-28 16:14

本文关键词：优秀自由式摔跤运动员的代谢组学研究　出处：《山西大学》2015年硕士论文　论文类型：学位论文

更多相关文章： 代谢组学 运动员 血清 自由式摔跤 训练

【摘要】：目的：通过对优秀自由式摔跤运动员(运动级别都为一级)训练周期血清代谢组学进行分析,同时结合传统生化指标检测,系统全面揭示自由式摔跤这一运动项目的代谢过程及其密切相关的代谢化合物,为科学的训练监控及其合理营养干预提供实验参考依据。方法：从山西省自由式摔跤队选取10名运动员(男、女各5人),于冬训开始的第一个训练周,分别在训练的前期、中期和后期采集3次血液样本,每次均自肘正中静脉取血液7m1,2m1放入抗凝管中用于检测血常规,剩余5m1全血离心后取血清,份用来检测血清肌酸激酶(CK)、血尿素(BU)和睾酮(T),另一份储存在-20℃直到进行核磁共振信号采集。通过1H NMR的代谢组学方法和多元统计分析寻找运动员不同训练阶段血清中的差异代谢标志物,数据均使用SPSS 19.0软件进行单因素方差分析。结果：(1)常规生化指标检测结果：男运动员血液WBC、RBC、Hb、Hct和MCV变化趋势一致,在训练后都出现下降,经过恢复之后重新升高,除了WBC恢复后的指标最低值略低于正常范围外,其余指标均处在正常的范围内。血清CK、BU在训练后出现显著升高(P0.05),CK在恢复后出现显著下降(P0.05)。T在训练后和恢复后也出现了先降低后升高的变化趋势,但其变化并没有显著性；女运动员RBC、Hb、Hct和MCV变化趋势一致,在训练后都出现下降,经过恢复之后重新升高,而WBC在训练后和恢复后则出现连续下降,除了WBC指标训练后和恢复后的指标最低值低于正常范围外,其余血液指标均处在正常的范围内。血清CK训练后与安静值相比,出现显著升高(P0.05),恢复后与训练后相比出现显著下降(P0.05)。T训练后与安静值相比出现显著下降(P0.05),在恢复后略有升高,但无显著性变化。BU在三个训练阶段的值先升高后降低,但均没有显著性变化；(2)代谢组学检测结果：男运动员血清代谢组信息中有6种差异代谢标志物(P0.05),分别是亮氨酸、异亮氨酸、缬氨酸、p-羟基丁酸、乳酸、马尿酸盐。其中与安静时相比,男运动员在训练后血清中的亮氨酸、异亮氨酸、缬氨酸、乳酸显著升高,而p-羟基丁酸、马尿酸盐显著下降,在恢复后血清中未发现有显著差异的代谢标志物。与训练后相比,男运动员在恢复后亮氨酸、异亮氨酸、缬氨酸、乳酸发生显著下降,而β-羟基丁酸、马尿酸盐没有显著性变化；女运动员血清代谢组信息中有3种差异代谢标志物(P0.05),分别是缬氨酸、丙酮酸、肌酸。其中与安静时相比,女运动员在训练后血清中缬氨酸显著下降,丙酮酸显著升高,肌酸无显著性变化,在恢复后血清中未发现有显著差异的代谢标志物。与训练后相比,女运动员在恢复后血清中缬氨酸和肌酸含量显著升高,丙酮酸含量显著下降。结论：代谢组学指标同血液常规生化指标均能对自由式摔跤运动员的训练监控提供有意义的信息,但是采用代谢组学指标相比于血液常规生化指标,更能准确、全面、深刻地反映运动员训练周期内机体的代谢过程和规律,对指导教练员和运动员科学训练和监控,进行合理营养补充具有重要意义。
[Abstract]:Objective: through to the elite free style wrestling (sports level for a training period) serum metabonomics analysis, combined with the detection of traditional biochemical indicators system fully reveal the metabolic process of wrestling the sport and metabolism of closely related compounds, provide experimental reference for the monitoring of the training and reasonable nutrition science the intervention. Methods: a total of 10 athletes from Shanxi province free wrestling team (male and female 5), in the beginning of winter of the first week of training, gather 3 blood samples in the training of the early middle and late respectively, each from the cubital vein blood into 7m1,2m1 anticoagulant tube for blood routine examination. The remaining 5m1 blood serum after centrifugation, samples for detection of serum creatine kinase (CK), blood urea (BU) and testosterone (T), another storage magnetic resonance signal acquisition in -20 until C. By means of 1H NMR metabonomics and multivariate statistical analysis, the serum metabolic markers in different training stages were found. Data were analyzed by one-way ANOVA using SPSS 19 software. Results: (1) the detection results of routine biochemical indexes: WBC, RBC, male athletes of blood Hb, Hct and MCV the same trend, there are decreased after training through recovery after re increased, in addition to the WBC after the recovery of the lowest index slightly lower than the normal range, other indexes were in normal range. The serum CK and BU were significantly increased after training (P0.05), and the CK decreased significantly after the recovery (P0.05). T after training and recovery also emerged after the trend first decreased and then increased, but the change was not significant; female athletes, Hb, Hct RBC and MCV the same change trend, there are decreased after training through recovery after re increased, while WBC after training and recovery appeared after a continuous decline in addition, the WBC index after training and recovery after the index is lower than the normal range, the blood indexes were in normal range. After training, the serum CK was significantly increased (P0.05) compared with the quiet value (P0.05) after the training. After T training, there was a significant decrease compared with the quiet value (P0.05), and a slight increase after recovery, but there was no significant change. BU first increased in the three stage of training value decreased, but there was no significant change; (2) findings of metabolic groups: male athletes serum metabolic information in 6 different metabolic markers (P0.05), respectively, leucine, isoleucine and valine, p- hydroxy butyric acid, lactic acid and hippuric acid salt. Which compared with the quiet, male athletes after training in serum leucine, isoleucine, valine, lactate increased significantly, while p- hydroxybutyric acid, hippurate decreased significantly, found no significant differences in metabolic markers in serum after recovery. Compared with male athletes in training, after the restoration of leucine, isoleucine, valine, lactate significantly decreased, while beta hydroxy butyric acid and hippuric acid salt did not change significantly; female athletes serum metabolic information in 3 different metabolic markers (P0.05), respectively, valine, pyruvate, creatine. Compared with the quiet time, the valine in the serum of female athletes decreased significantly after training, pyruvate increased significantly, and creatine did not change significantly. No significant metabolic markers were found in the serum after recovery. Compared with the training, the content of valine and creatine in the serum of female athletes increased significantly, and the content of pyruvic acid decreased significantly. Conclusion: metabonomics indexes with conventional biochemical indexes of blood of free style wrestling training monitoring provides meaningful information, but the metabonomics index compared to the conventional biochemical index of blood, more accurately and comprehensively and profoundly reflect the metabolic process and regularity of athletes in the training cycle of coaches and body. Athletes scientific training and monitoring, reasonable nutritional supplement has important significance.
【学位授予单位】：山西大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：G886.2;G804.7

【参考文献】