运动对大鼠骨骼肌细胞凋亡及钙离子含量的影响

发布时间：2018-05-12 04:39

本文选题：运动 + 细胞凋亡　；参考：《动物医学进展》2017年09期

【摘要】：探讨运动对大鼠骨骼肌细胞凋亡及钙离子含量的影响。将大鼠分为对照组、一般运动组和强运动组,对照组大鼠正常活动,一般运动组大鼠跑台速度为18m/min,强运动组大鼠跑台速度为25m/min,一般运动组和强运动组大鼠均运动至力竭。分别检测大鼠比目鱼肌和胫骨前肌细胞凋亡情况,大鼠血清和大鼠后肢组织匀浆上清中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和钙离子水平,大鼠肌肉组织中Bax、Bcl-2表达水平。结果发现,一般运动组大鼠比目鱼肌中细胞凋亡率和SOD活性均明显高于对照组,差异极显著(P0.01)。强运动组大鼠比目鱼肌中的SOD活性明显低于一般运动组,差异极显著(P0.01)。试验组大鼠血清中MDA水平均明显低于对照组,差异极显著(P0.01)。一般运动组大鼠胫骨前肌和比目鱼肌中MDA水平均明显高于对照组和强运动组,差异极显著(P0.01)。一般运动组大鼠比目鱼肌中钙离子水平和Bax表达水平明显高于对照组,差异显著(P0.05)。一般运动组大鼠比目鱼肌中Bcl-2表达水平明显低于对照组,差异极显著(P0.01)。表明一般运动比高强度运动更易导致比目鱼肌细胞凋亡,作用机制可能与钙离子浓度、氧自由基水平、Bax、Bcl-2有关。
[Abstract]:To investigate the effect of exercise on apoptosis and calcium content of skeletal muscle cells in rats. The rats were divided into three groups: control group, general exercise group and strong exercise group. The normal activity of rats in the control group was 18 m / min in general exercise group, 25 m / min in strong exercise group, and exhaustion in general exercise group and strong exercise group. The apoptosis of rat soleus muscle and tibial anterior muscle, the activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and MDAs and the level of calcium ion in the supernatant of rat serum and hind limb homogenate were measured respectively. The results showed that the apoptosis rate and SOD activity of soleus muscle in general exercise group were significantly higher than those in control group (P 0.01). The activity of SOD in the soleus muscle in the strong exercise group was significantly lower than that in the normal exercise group (P 0.01). The serum MDA levels in the experimental group were significantly lower than those in the control group (P 0.01). The levels of MDA in the anterior tibial muscle and soleus muscle in the general exercise group were significantly higher than those in the control group and the strong exercise group (P 0.01). The level of Ca ~ (2 +) and Bax expression in soleus muscle in general exercise group was significantly higher than that in control group (P 0.05). The expression of Bcl-2 in soleus muscle in general exercise group was significantly lower than that in control group (P 0.01). The results showed that general exercise was more likely to induce the apoptosis of soleus muscle cells than high-intensity exercise, and the mechanism might be related to the concentration of calcium ion and the level of oxygen free radicals (OFR) and BaxanBcl-2.
【作者单位】：河北北方学院公共体育部;河北北方学院附属第二医院药剂科;
【分类号】：G804.7

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