不同运动方式对骨骼肌PGC-1α的激活作用

发布时间：2018-05-26 21:48

本文选题：向心运动 + 离心运动　；参考：《河北师范大学》2017年硕士论文

【摘要】：肥胖全球流行已成当前公共健康问题,防肥减肥任务重大。其中运动对减肥的防控也是研究热点之一。PGC-1α是细胞能量代谢与脂肪氧化调节的关键信号,探索不同的运动方式对PGC-1α的激活,对运动减肥方案的制定具有重要的意义。研究目的:观察在相同摄氧量强度下,不同肌肉收缩形式(向心和离心)的运动对PGC-1α的激活作用,进而揭示相同摄氧量状态下,不同运动方式对运动后0-48小时内机体能量代谢信号的作用,以及是否存在时相性变化。研究方法:实验第一部分取雄性SD大鼠24只,随机分为向心运动组(E组)和离心运动组(L组),各组分别为12只大鼠。先进行三天的适应性训练后,休息24小时。E组大鼠进行平坡跑运动(坡度0°,跑速20m/min,时间15min),L组大鼠进行下坡跑运动(坡度-5°,跑速Xm/min,时间15min)。实验中调整L组的跑速直至摄氧量与E组一致。所得到的跑速为实验第二部分L组大鼠的跑速X。实验第二部分取雄性SD大鼠66只,随机分成11组,每组6只,分别为对照组(C),向心运动后E0、E6、E12、E24、E48小时组。离心运动后L0、L6、L12、L24、L48小时组。向心运动组和离心运动组分别进行三天的适应性训练。休息24小时。E组大鼠进行平坡跑运动(坡度0°、跑速20m/min,时间60min)。L组大鼠进行下坡跑运动(坡度-5°、跑速Xm/min,时间60min)。跑速X为实验第一部分确定的跑速。各组大鼠一次性跑台运动后取材,采用Western Blot检测腓肠肌和比目鱼肌中PGC-1α的蛋白达情况,采用透射电子显微镜观察大鼠比目鱼肌肌纤维微细结构的变化。研究结果:(1)E组与L组大鼠在跑速为20m/min时摄氧量无统计学差异(P0.05)。(2)L组肌纤维微细结构的变化有大于E组的趋势,且持续的时间较长。(3)E组与L组大鼠在相同摄氧量强度下不同肌肉收缩方式(向心和离心)运动后,腓肠肌和比目鱼肌PGC-1α蛋白表达量与C组相比,E组均有增加,但无统计学差异(P0.05),L组PGC-1α蛋白表达量明显增加,且在0h、48h具有非常显著性差异(P0.01),但在6h、24h无统计学差异(P0.05),在12h具有显著性差异(P0.05)。E组腓肠肌和比目鱼肌PGC-1α蛋白表达量与L组相比,在0h、12h、48h具有显著性差异(P0.05),在6h、24h无统计学差异(P0.05)。结论:摄氧量相同的一次性跑台运动后,离心收缩可显著性提升大鼠骨骼肌PGC-1α蛋白的表达,且持续时间较长,呈现运动后即刻和48小时双高峰现象。而向心收缩有增加趋势但无统计学意义。
[Abstract]:Obesity has become a global public health problem and the task of preventing obesity and losing weight is very important. Among them, the prevention and control of exercise on weight loss is also one of the research hotspots. PGC-1 伪 is the key signal of cell energy metabolism and lipid oxidation regulation. It is of great significance to explore the activation of PGC-1 伪 in different exercise modes and to make the exercise weight loss program. Objective: to observe the activation of PGC-1 伪 by the exercise of different muscle contraction forms (concentric and centrifugal) under the same oxygen uptake intensity. The effects of different exercise modes on energy metabolism signal in 0-48 hours after exercise, and whether there are temporal changes. Methods: in the first part of the experiment, 24 male Sprague-Dawley rats were randomly divided into two groups: concentric exercise group (n = 12) and centrifugal exercise group (n = 12). After three days of adaptive training, the rats in group E were given downhill running (slope 0 掳, running speed 20 m / min, time 15 min) and downhill running (slope -5 掳, running speed X m / min, time 15 min). In the experiment, the running speed of group L was adjusted until oxygen uptake was the same as that in group E. The second part of the experiment was the running speed of the L group rats. In the second part, 66 male Sprague-Dawley rats were randomly divided into 11 groups with 6 rats in each group. After centrifugation, L _ 0, L _ 6, L _ (12) and L _ (24) L _ (48) h group. The centripetal exercise group and the centrifugal exercise group were trained for 3 days respectively. The rats in the rest group (0 掳slope, 20 m / min running speed) underwent downhill running (slope -5 掳, running speed X m / min, time 60 mins) in the rest group (0 掳, 20 m / min), while the rats in the 60min).L group underwent downhill running (slope -5 掳, running speed X m / min, time 60 min). Running speed X is the speed determined in the first part of the experiment. The contents of PGC-1 伪 protein in gastrocnemius muscle and soleus muscle were detected by Western Blot and the changes of fiber microstructures in soleus muscle were observed by transmission electron microscope. Results there was no significant difference in oxygen uptake between group E and group L when the running speed was 20m/min. The changes of muscle fiber microstructures in group E were larger than those in group E. The expression of PGC-1 伪 protein in gastrocnemius muscle and soleus muscle of group E and group L increased after exercise of different muscle contraction modes (concentric and centrifugal) at the same oxygen uptake intensity, compared with group C, and the expression of PGC-1 伪 protein in gastrocnemius muscle and soleus muscle of group E was significantly higher than that in group C, and the expression of PGC-1 伪 protein in gastrocnemius muscle and soleus muscle was increased compared with group C. However, there was no significant difference in the expression of PGC-1 伪 protein between the two groups, and the expression of PGC-1 伪 protein in gastrocnemius muscle and soleus muscle was significantly higher than that in group L at 0 h or 48 h, but there was no significant difference at 6 h or 24 h. At 12 h, the expression of PGC-1 伪 protein in gastrocnemius muscle and soleus muscle in group E was significantly higher than that in group L, and the expression of PGC-1 伪 protein in gastrocnemius muscle and soleus muscle in group E was significantly higher than that in group L at 12 h. There was significant difference at 12h ~ 48h (P 0.05A), but there was no significant difference (P 0.05) at 6h ~ 24h. Conclusion: centrifugal contraction can significantly promote the expression of PGC-1 伪 protein in skeletal muscle of rats after a single treadmill exercise with the same oxygen intake, and it lasts for a long time, showing a double peak phenomenon immediately and 48 hours after exercise. There was an increasing trend of concentric contraction, but no statistical significance.
【学位授予单位】：河北师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

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