不同运动对糖尿病大鼠主动脉平滑肌表型转化的影响

发布时间：2018-05-27 15:26

本文选题：平滑肌表型转化 + 糖尿病　；参考：《成都体育学院》2017年硕士论文

【摘要】：目的:了解耐力与抗阻运动对Ⅱ型糖尿病大鼠主动脉平滑肌表型转化的影响及可能机制。方法:雄性SD大鼠随机分为普通饲料组(pC)和高糖高脂饲料组(pD),7周后pD组腹腔注射链脲佐菌素,72h后非禁食血糖(NFBG)≥16.7mmol/L且出现胰岛素抵抗者纳入糖尿病组。此后,将pC组随机分为空白对照组(C组)、单纯耐力运动组(CA组)和单纯抗阻运动组(CR组),糖尿病组分为糖尿病模型组(D组)、糖尿病耐力运动组(DA组)和糖尿病抗阻运动组(DR组)。CA、DA组无负重跑台运动,CR、DR组负重爬梯训练,每周5天,共8周。8周后酶化学法测试各组血糖血脂指标,酶联免疫检测主动脉平滑肌肌动蛋白α(α-SM-actin)、平滑肌肌球蛋白重链(SM-MHC)、平滑肌22α(SM22α)、心肌素(Myocardin)、血清应答因子(SRF)和Krüppel样转录因子4(KLF4)的表达,另取部分主动脉行HE和Masson染色观察组织病理变化。结果:1.pD组36只大鼠中有26只达糖尿病标准,成模率72.2%。2.成模8周后,与C组相比,D组大鼠主动脉平滑肌纤维走向异常,胶原容积分数(CVF)显著上升,颈动脉血压(MCP)显著上升,空腹血糖(FBG)、胰岛素抵抗指数(HOMA-IRI)、甘油三酯(TG)、低密度脂蛋白(LDL)等显著上升,胰岛素(FINS)显著下降,α-SM-actin、SM-MHC、SM22α、Myocardin及SRF表达显著下降,KLF4显著上升。3.运动8周后,与D组相比,DA组大鼠CVF下降,MCP下降,FBG、TG、LDL等表达显著下调,FINS显著上调,α-SM-actin、SM22α及Myocardin表达显著上调,KLF4表达略下调;DR组CVF下调,MCP无明显变化,FBG、TG、LDL等表达显著下调,FINS显著上调,α-SM-actin、SM22α及Myocardin表达略上调,但无显著差异。DA组与DR组相比,除在血糖控制及改善胰岛素抵抗上DR组优于DA组外,其余指标DA组均优于DR组。结论:1.糖尿病成模8周后,大鼠发生颈动脉血压升高、主动脉胶原纤维沉积及表型由收缩型向合成型转变。2.8周耐力或抗阻运动能降低糖尿病大鼠血糖、血脂水平,缓解胰岛素抵抗,减少主动脉胶原纤维沉积,减轻平滑肌细胞由收缩型向合成型的转变;耐力运动在降血脂、降血压及减轻平滑肌细胞由收缩型向合成型的转变上优于抗阻运动;抗阻运动在降血糖和改善胰岛素抵抗上优于耐力运动。3.8周耐力或抗阻运动对糖尿病大鼠主动脉平滑肌细胞表型调控作用的差异可能与不同运动提高Myocardin的表达程度不同有关。
[Abstract]:Aim: to investigate the effects of endurance and resistance exercise on phenotypic transformation of aortic smooth muscle in type 2 diabetic rats. Methods: male Sprague-Dawley rats were randomly divided into two groups: normal diet group (PC) and high glucose and high fat diet group. Since then, The PC group was randomly divided into control group (C group), endurance exercise group (CA group) and resistance exercise group (CR group). Diabetes group was divided into diabetic model group (D group), diabetic endurance exercise group (DA group) and diabetic resistance exercise group (DM group). The training of weight-bearing climbing ladder in group R, Dr, group C, C, D, D, D, C, D, D, C, D, D, C, D, D, C, D, D, C. The blood glucose and blood lipid were measured by enzyme chemistry after 8 weeks, 5 days a week. The expression of smooth muscle actin 伪 (伪 -SM-actinine), smooth muscle myosin heavy chain (SM-MHCC), smooth muscle 22 伪 ppel SM22 伪, myocardial myocardinine, serum response factor (SRF) and Kr 眉 ppel like transcription factor 4 (KLF4) were detected by enzyme-linked immunosorbent assay (Elisa). The histopathological changes were observed by HE and Masson staining in some aorta. Results: 1. 26 of 36 rats in PD group met the standard of diabetes mellitus, and the rate of model formation was 72. 2%. After 8 weeks, compared with group C, the aortic smooth muscle fibers in group D were abnormal, the collagen volume fraction (CVF) was significantly increased, and the carotid blood pressure was significantly increased. Fasting blood glucose (FBG), insulin resistance index (HOMA-IRI), triglyceride (TG), low density lipoprotein (LDL), etc., decreased significantly, and the expression of 伪 -SM-actinin SM-MHCSSM22 伪 and SRF increased significantly (KLF4). After eight weeks of exercise, Compared with group D, the expression of CVF in DA group was decreased and the expression of FBGA-TGN LDL was significantly down-regulated. The expression of 伪 -SM-actinine SM22 伪 and Myocardin was up-regulated slightly in Dr group. There was no significant change in CVF down-regulation of CVF in Dr group. The expression of FBGG-LDL was significantly down-regulated, and the expression of 伪 -SM-actinine SM22 伪 and Myocardin was slightly up-regulated. However, there was no significant difference between DA group and Dr group, except that Dr group was superior to DA group in controlling blood glucose and improving insulin resistance, and all other indexes in DA group were superior to Dr group. Conclusion 1. After 8 weeks of diabetic model, carotid blood pressure increased, aortic collagen fiber deposition and phenotype changed from contraction type to synthetic type. 2.8-week endurance or resistance exercise could reduce blood glucose, blood lipid level and relieve insulin resistance in diabetic rats. Reducing the deposition of collagen fibers in aorta and reducing the transition of smooth muscle cells from contractile type to synthetic type, endurance exercise was superior to resistance exercise in lowering blood lipids, lowering blood pressure and reducing the transition of smooth muscle cells from contractile type to synthetic type. The difference of resistance exercise in lowering blood glucose and improving insulin resistance is better than endurance exercise at 3.8-week endurance or resistance exercise on the phenotypic regulation of aortic smooth muscle cells in diabetic rats, which may be related to the different degree of Myocardin expression increased by different exercise.
【学位授予单位】：成都体育学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7

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