运动产生的8-羟鸟嘌呤对小鼠骨骼肌成肌细胞增殖和分化的影响

发布时间：2018-06-29 20:47

本文选题：8-羟基鸟嘌呤 + 小鼠骨骼肌成肌细胞　；参考：《吉林大学》2017年硕士论文

【摘要】：骨骼肌占人体体重的40%,其在运动、呼吸、动作维持、体温调节、代谢调节等功能中有着不可替代的作用。肌肉生成是一系列复杂而精细的过程,主要由四个方面,即活化肌肉干细胞、肌细胞增殖分化、形成肌细胞、细胞相互融合继而形成多核的肌管。大负荷的运动促使骨骼肌生成活性氧,其可促进骨骼肌肌肉生成,但是直接的证据很少。本文探究运动产生的8-羟鸟嘌呤对小鼠骨骼肌成肌细胞C2C12增值分化的影响。首先进行分组训练,一组为安静组,一组为进行下坡跑运动的运动组,运动组保持其运动速度为16m/min,运动坡度为-16o,运动的时间为持续100分钟运动,5分钟休息,再运动100分钟,实验结果通过免疫组化的方法,验证运动与氧化损伤的正相关性,在此基础之上,基于C2C12增值分化模型,通过有无添加8-羟鸟嘌呤,建立刺激组和未加刺激组。显微镜观察各时期细胞形态,计算各时期细胞数;通过实验,检测相关成肌分子MyoD、M-Cadherin和MyoG在mR NA方面的表达水平。通过研究得出以下结论:1.通过进行免疫组化实验,发现运动组的实验动物骨骼肌产生大量的8-oxo G,表明大负荷运动下,骨骼肌产生的运动损伤程度较为严重。2.通过对刺激组和未加刺激组的两组细胞进行细胞计数、发现了刺激组和未加刺激组在细胞密度为80%时,刺激组细胞产生的分裂相较之未加刺激组明显增多(P0.01),这说明了运动产生的8-oxoG能明显地促进骨骼肌细胞的增殖。3.通过对刺激组和未加刺激组的细胞进行RT-PCR,发现了刺激组在加入刺激物8-oxoG后,对应的三种成肌分子MyoD、M-Cadherin和MyoG的mRNA表达明显增强(P0.05),这些说明了运动产生的8-oxoG对骨骼肌成肌细胞的分化具有显著地促进作用。
[Abstract]:Skeletal muscle accounts for 40% of body weight, which plays an irreplaceable role in the functions of exercise, respiration, movement maintenance, thermoregulation, metabolic regulation, and so on. Muscle generation is a series of complex and fine processes, which mainly consist of four aspects, namely, activating muscle stem cells, proliferating and differentiation of muscle cells, forming myocytes, and forming multicore cells to form each other. Myotubes. Heavy load movement causes skeletal muscle to produce reactive oxygen species, which can promote skeletal muscle formation, but there is little direct evidence. This article explores the effect of 8- hydroxyguanine on the C2C12 increment and differentiation of skeletal myoblasts in mice. First, group training, a group of quiet groups, and a group for downhill running In the dynamic group, the movement group keeps its speed of 16m/min, the motion slope is -16o, the time of the movement is 100 minutes, 5 minutes rest and then 100 minutes. The experimental results verify the positive correlation between the movement and the oxidative damage through the immunohistochemical method. On this basis, it is based on the C2C12 value-added differentiation model, through the addition of 8- hydroxyl birds Purine, set up the stimulation group and the non stimulation group. The cell morphology of each period was observed by microscope and the number of cells in each period was calculated. Through the experiment, the expression level of MyoD, M-Cadherin and MyoG in mR NA was detected. Through the study, the following conclusions were obtained: 1. by the immunohistochemical experiment, the skeletal muscle production of the experimental animals was found. A large number of 8-oxo G showed that the degree of motor damage caused by skeletal muscle was more serious under the heavy load movement, and.2. was counted by the cell count of two groups of cells in the stimulation group and the non stimulation group. It was found that when the cell density was 80% in the stimulation group and the non stimulation group, the cells produced by the stimulation group were significantly more than those in the non stimulation group (P0.01 This shows that the 8-oxoG produced by the exercise can obviously promote the proliferation of skeletal muscle cells through the RT-PCR of the cells in the stimulation group and the non stimulation group. It is found that after the stimulation of 8-oxoG, the corresponding three kinds of myoblasts, MyoD, M-Cadherin and MyoG, are obviously enhanced (P0.05). These indicate the 8-o of the movement generated 8-o. XoG has a significant effect on the differentiation of skeletal myoblasts.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

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