有氧运动对大鼠不同时期脑衰老及学习记忆能力与额叶BDNF表达的影响

发布时间：2018-07-03 05:40

本文选题：有氧运动 + 脑衰老　；参考：《成都体育学院》2017年硕士论文

【摘要】：目的:通过观察有氧运动对SD大鼠衰老模型空间学习记忆能力及额叶脑源性神经营养因子(BDNF)表达的影响,探讨有氧运动对大鼠脑衰老的影响及作用机理。方法:3月龄SD雄性大鼠共54只,随机分成6组:生理盐水注射对照组(NNa组)、D-半乳糖注射致衰老组(ND组)、D-半乳糖注射致衰老前运动组(SD组)、D-半乳糖注射致衰老过程中运动组(NDs组)、D-半乳糖注射致衰老后运动组(NDS组)以及D-半乳糖注射致衰老后自然喂养组(NDN),每组9只(n=9)。SD组于衰老造模前进行4周有氧运动干预,NDs组于衰老造模过程中进行6周有氧运动干预,NDS组于衰老造模后进行4周有氧运动干预,NDN组于衰老造模后不进行运动干预。选用Morris水迷宫系统训练并检测各组大鼠定位航行与空间探索能力。实验结束之后,断头处死,分离大鼠额叶。检测不同衰老时期大鼠超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-PX)的活性以及丙二醛(MDA)的含量。应用免疫组化、Real time PCR以及Westren-Blotting技术分别检测额叶BDNF基因mRNA和蛋白的表达。结果:1.自由基检测结果:ND组比NNa组GSH-PX和SOD的活性显著降低(P0.05),MDA的含量显著增加(P0.05);NDs组比ND组GSH-PX和SOD的活性显著增加(P0.05),MDA的含量显著降低(P0.05);SD组比ND组SOD、GSH-PX的活性以及MDA的含量均未出现显著性变化(P0.05);NDS组比NDN组GSH-PX和SOD的活性显著增加(P0.05),MDA的含量显著降低(P0.05);NDN组比ND组GSH-PX和SOD的活性也未出现显著的差异(P0.05),MDA的含量显著增加(P0.05);NDs组比NDS组GSH-PX和SOD的活性显著升高(P0.01),MDA的含量显著降低(P0.01)。2.空间学习能力检测结果:(1)平台穿越次数:ND组比NNa组穿越原站台次显著减少(P0.05);NDs组比ND组显著增加(P0.05),SD组与ND组之间未出现显著的差异(P0.05);NDN组与NDS组也未出现显著的差异(P0.05)。(2)原平台象限的游泳路程与总路程的比值:ND组显著低于NNa组(P0.01);NDs组显著高于ND组(P0.01),SD组与ND组无显著差异(P0.05);NDN组与NDS组也没有显著差异(P0.05)。3.BDNF表达情况:ND组比NNa组BDNF蛋白表达量显著下调(P0.05);NDs组比ND组BDNF mRNA及蛋白表达量均显著下调(P0.01),SD组BDNF mRNA及蛋白表达量与ND组相比未出现显著差异(P0.05)。结论:1.通过6周D-半乳糖注射成功构建了亚急性衰老大鼠模型。2.脑衰老过程中进行有氧运动干预可以提高大鼠的学习记忆能力,上调额叶BDNF的表达,说明衰老过程中进行有氧运动干预能够延缓脑衰老。3.额叶BDNF蛋白表达量与学习记忆呈线性正相关,提示额叶BDNF表达量的上调是改善学习记忆能力的机制之一。
[Abstract]:Objective: to observe the effects of aerobic exercise on the spatial learning and memory ability and the expression of BDNF in the frontal lobe of SD rats, and to explore the effect and mechanism of aerobic exercise on brain aging in SD rats. Methods 54 male Sprague-Dawley rats aged 3 months were selected. The rats were randomly divided into 6 groups: normal saline injection control group (NNa group), D-galactose injection induced senescence group (ND group), D-galactose injection induced aging exercise group (SD group), D-galactose injection induced senescence process, exercise group (NDs group), D-galactose injection induced senescence. Elderly exercise group (NDS group) and natural feeding group (NDN) induced by D-galactose injection. Nine rats in each group (nun9). SD group were treated with aerobic exercise for 4 weeks before aging model making. NDs group underwent 6 weeks aerobic exercise intervention during aging model making process. Aerobic exercise intervention was performed for 4 weeks after aging model making. No exercise intervention was carried out in NDN group after aging modeling. Morris water maze system was used to train and measure the ability of navigation and space exploration. After the experiment, the rats were killed and the frontal lobes were separated. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and the content of malondialdehyde (MDA) in different aging rats were determined. The expression of BDNF gene mRNA and protein in frontal lobe was detected by immunohistochemistry real time PCR and Westren-Blotting respectively. The result is 1: 1. Results the activity of GSH-PX and SOD decreased significantly (P0.05) the activity of GSH-PX and SOD in NDs group was significantly lower than that in ND group (P0.05), the activity of GSH-PX and SOD in NDs group was significantly higher than that in ND group (P0.05), the activity of GSH-PX and MDA in SD group was significantly lower than that in ND group (P0.05) and the content of MDA in SD group was not significantly higher than that in ND group. The activity of GSH-PX and SOD in NDS group was significantly higher than that in NDN group (P0.05) the content of MDA in NDS group was significantly lower than that in NDN group (P0.05) there was no significant difference in GSH-PX and SOD activity between NDN group and ND group (P0.05). (P0.05) the activity of GSH-PX and SOD in NDS group was significantly higher than that in NDS group (P0.05). The content of MDA decreased significantly (P0.01). The results of spatial learning ability test: (1) the number of platform traversing through the original platform was significantly lower in the N _ ND group than in the NNA group (P0.05). (P0.05) there was no significant difference between the SD group and the ND group (P0.05). There was no significant difference between the NDN group and the NDS group. (P0.05) there was no significant difference between the NDN group and the NDS group. (P0.05) there was no significant difference between the SD group and the ND group (P0.05). (P0.05) the ratio of swimming distance to total distance in the original platform quadrant in ND group was significantly lower than that in NNa group (P0.01). There was no significant difference between SD group and ND group (P0.05). The expression of BDNF mRNA and protein in NDs group was significantly lower than that in ND group (P0.01). There was no significant difference in BDNF mRNA and protein expression between SD group and ND group (P0.05). Conclusion 1. Subacute aging rat model was successfully established by injection of D-galactose at 6 weeks. Aerobic exercise intervention during brain aging can improve the learning and memory ability of rats and up-regulate the expression of BDNF in frontal lobe, indicating that aerobic exercise intervention during aging can delay brain aging. 3. The expression of BDNF protein in frontal lobe was positively correlated with learning and memory, suggesting that the up-regulation of BDNF expression in frontal lobe is one of the mechanisms to improve learning and memory ability.
【学位授予单位】：成都体育学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7

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