跑台训练对脑外伤大鼠运动功能以及脑内线粒体的影响

发布时间：2018-07-25 13:56

【摘要】：研究背景:颅脑外伤是世界范围的公共健康问题,很多脑外伤患者会长期遗留不同的程度的神经功能障碍,如运动功能障碍、言语功能障碍和认知功能障碍等,严重影响着患者的日常生活以及自理能力,目前没有一种有效的方法或者药物来完全治愈这类疾病,但康复治疗可以明显改善颅脑外伤患者的神经功能。然而康复治疗的机制尚未被完全阐明。本课题拟从运动康复训练改善颅脑外伤后大鼠的运动功能这一视角,初步探讨跑台运动训练改善颅脑外伤大鼠运动功能的线粒体相关机制。研究方法:将96只成年SD大鼠随机分为对照组、脑外伤组和外伤运动组,对外伤组和外伤运动组大鼠制作国际上常用的大鼠的颅脑外伤模型:控制性皮质冲击损伤模型(Controlled cortical impact,CCI)。造模后1天外伤运动组开始进行为期两周的的跑台运动训练,并观察一系列行为学表现(包括大鼠神经功能损伤、错步试验等);伊文斯蓝(EB)染色检测大鼠血脑屏障通透性;干湿法检测大鼠大脑含水量;透视电镜观察各组大鼠线粒体形态;通过2,3,5—氯化三苯基四氮唑(TTC)染色检测各组大鼠脑组织体积缺失;通过Western blot检测受伤侧大脑皮质相关蛋白(HIF-1,Bcl-2,Bax,LC3B,mfn1,mfn2)的表达情况。结果:外伤运动组体重一直大于外伤组且在造模第7天两者有统计学差异(P0.05);外伤运动组大鼠的神经损伤评分在造模后第7天、10天和14天的得分和脑外伤组得分之间具有统计学意义(P0.05),外伤运动组大鼠的神经损伤评分在造模后第7天和第10天与脑外伤组对比具有显著性统计学差异(P0.01);外伤运动组和脑外伤组大鼠的错步实验中步速在第7天和第10天之间存在着统计学差异(P0.05)且外伤运动组的步速要高于脑外伤组大鼠的步速,造模后第14天,外伤运动组大鼠的步速要明显高于脑外伤组大鼠的步速,二者之间存在着显著性的统计学差异(P0.01);在造模后14天,脑外伤组和外伤运动组大鼠脑缺损相比,脑截面缺损面积较大,缺损体积也较大,存在统计学差异(P0.05);外伤组大鼠的大脑含水量远远高于对照组和外伤运动组(P0.05);外伤组的脑内伊文思蓝含量高于外伤运动组和对照组,和外伤运动组相比具有统计学差异(P0.05);外伤运动组脑线粒体的水肿状态明显好于外伤组;外伤运动组相比脑外伤组,Western Blot结果显示HIF-1表达下调,Bcl-2表达上调,Bax表达下调,LC3B,mfn1,mfn2表达上调。结论:跑台运动训练可以在一定程度上促进脑外伤大鼠身体机能的修复,改善大鼠的运动功能;跑台运动训练改善脑外伤急性期大鼠脑水肿状况,对血脑屏障起到修复作用;跑台运动训练有利于改善大脑脑实质坏死的情况,改善脑功能;运动训练有利于促进大鼠脑内细胞的自噬,改善线粒体的水肿和缺氧情况,促进脑内损伤细胞的修复,改善脑功能。
[Abstract]:Background: craniocerebral injury is a worldwide public health problem. Many patients with traumatic brain injury have long been left with different degrees of neurological dysfunction, such as motor dysfunction, speech dysfunction and cognitive dysfunction. At present, there is no effective method or medicine to cure these diseases, but rehabilitation therapy can obviously improve the neurological function of patients with craniocerebral trauma. However, the mechanism of rehabilitation has not been fully clarified. From the point of view of improving the motor function of the rats after craniocerebral trauma, the mechanism of mitochondria related to the improvement of motor function in rats with craniocerebral trauma by treadmill training was discussed. Methods: 96 adult Sprague-Dawley rats were randomly divided into three groups: the control group, the brain injury group and the traumatic exercise group. One day after the injury, the traumatic exercise group began to undergo two weeks of treadmill exercise training, and observed a series of behavioral manifestations (including nerve function injury, staggered test, etc.), and Evans blue (EB) staining was used to detect the blood-brain barrier permeability in rats. Brain water content was measured by dry-wet method, mitochondria morphology was observed by fluoroscopy electron microscope, and brain tissue volume deficiency was detected by (TTC) staining of 3-triphenyl tetrazolium chloride in each group. The expression of cortical associated protein (HIF-1, Bcl-2) in injured side was detected by Western blot. Results: the body weight of the trauma exercise group was higher than that of the injury group and there was a statistical difference between the two groups on the 7th day (P0.05), and the nerve injury score of the trauma exercise group was 10 and 14 days after the establishment of the model and the score of the brain injury group was higher than that of the brain injury group (P0.05). There was significant statistical difference between the two groups (P0.05). The neurological injury score of the rats in the traumatic exercise group was significantly different from that in the brain injury group on the 7th and 10th day after the model was made (P0.01); in the staggered step experiment of the rats in the traumatic exercise group and the brain injury group, there was a significant difference between the two groups (P0.01). There was statistical difference between 7 and 10 days of walking speed (P0.05), and the walking speed of traumatic exercise group was higher than that of brain injury group. On the 14th day after modeling, the walking speed of the rats in the traumatic exercise group was significantly higher than that in the brain injury group (P0.01), and there was a significant difference between the two groups (P0.01), and on the 14th day after the establishment of the model, the brain defect of the rats in the traumatic exercise group and that in the traumatic exercise group were significantly higher than that in the control group (P0.01). The area of brain section defect is larger, the defect volume is larger, there is statistical difference (P0.05); the brain water content of the trauma group is much higher than that of the control group and the trauma exercise group (P0.05); the content of Evans blue in the brain of the trauma group is higher than that of the trauma exercise group and the control group. Compared with the traumatic exercise group, the brain mitochondria edema status in the traumatic exercise group was significantly better than that in the traumatic exercise group (P0.05). The results of Western Blot showed that the down-regulation of HIF-1 expression and the down-regulation of the expression of BFN1mfn1mfn2.The results showed that the expression of HIF-1 down-regulated and down-regulated the expression of mfn1mfn1mfn2. Conclusion: treadmill training can improve the physical function of brain injury rats to some extent, improve the motor function of rats, and improve the brain edema and blood brain barrier in rats with acute brain injury. Treadmill training can improve the necrosis of brain parenchyma, improve brain function, promote autophagy of rat brain cells, improve mitochondria edema and hypoxia, and promote the repair of injured cells in brain. Improve brain function.
【学位授予单位】：上海体育学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7

【相似文献】