五周亚极限强度运动对StAR及其调控因子的影响

发布时间：2018-08-17 14:35

【摘要】：目的:通过研究胆固醇转运蛋白StAR相关调控因子因子PKA、pCREB和SF-1表达的时序性变化,探讨五周亚极限强度运动对大鼠血睾酮水平及StAR蛋白的影响,寻找运动性低睾酮的原因,为防治运动性低睾酮提供依据。方法:建模实验:30只8周雄性SD大鼠分成A、B、C三组,A组、B组分别负重5%和6%体重进行4min×5次游泳训练,间隔90秒,上、下午各进行一次训练,每周6天,C组安静饲养,持续五周。训练结束48h后取材待测,检测大鼠CK、BU、T和StAR mRNA变化情况。正式实验:90只8周雄性SD大鼠分为运动1周(T1)、运动2周(T2)、运动3周(T3)、运动4周(T4)、运动5周(T5)等5个运动干预组和运动前、后2个对照组(C0,C5)。运动组负重6%体重上述训练方案进行训练,训练结束48h后取材待测,采用Western Blot、RT-PCR等技术检测StAR、PKA、pCREB、SF-1等蛋白及基因mRNA变化情况。结果:建模实验:1、A组和B组运动结束3min后乳酸水平为均显著高于C组(P0.01),且A与B组之间存在显著差异(P0.01)。2、与对照组相比,运动干预组CK和BU均显著增加(p0.01),但组间无显著差异。3、B组大鼠StAR mRNA表达与C组相比显著下降(p0.05)。正式实验:1、运动干预组血清睾酮水平前三周下降较缓慢,随后下降幅度增大,T5组血清睾酮水平与C0组相比下降了70%(p0.01)。LH出现曲折下降,第五周LH水平下降更显著与C0相比下降了41%,差异均有显著性(p0.01)。2、运动干预组训练后3min乳酸水平显著上升。其变化趋势为:前三周上升幅度较大(p0.01),后两周则维持在较高水平且略有上升(p0.05)。SOD活性不断下降。第五周下降至C0组SOD活性的77%(p0.01)。MDA含量前三周略有波动,第四周、五周显著上升,较C0组MDA变化差异非常显著(p0.01)。3、大鼠睾丸组织PKA、pCREB、SF-1蛋白表达在前三周保持稳定,随后显著下降(p0.05)。4、运动干预组StAR mRNA表达在前三周变化不显著,但T5组大鼠mRNA表达与安静组C0相比差异出现了显著性(p0.05)。第一周SF-1 mRNA较C0组略有上升,随后逐渐下降至第五周差异具有显著性(p0.01)。结论:1、五周亚极限强度运动引起睾丸组织过氧化水平提高、血清睾酮、睾丸间质细胞内StAR及其相关调控因子PKA、pCREB、SF-1等指标下降。2、StAR相关调控因子被抑制导致StAR mRNA表达下降进而引起胆固醇转运受限可能是加速睾酮下降的原因。
[Abstract]:Aim: to study the temporal changes of the expression of PKAP-CREB and SF-1 associated with cholesterol transporter (StAR), and to explore the effects of 5-week sub-limit intensity exercise on serum testosterone level and StAR protein in rats, and to find out the causes of exercise low testosterone. To provide evidence for prevention and treatment of exercise low testosterone. Methods: thirty 8-week male Sprague-Dawley rats were divided into three groups: group A (weight bearing 5%) and group B (6% body weight). The rats in group A were given 4min 脳 5 swimming exercises at intervals of 90 seconds. The rats were trained once in the afternoon. The rats in group C were kept quietly for five weeks. The changes of CKBU T and StAR mRNA were detected 48 hours after training. Formal experiment: 90 male Sprague-Dawley rats at 8 weeks were divided into 5 exercise intervention groups: exercise 1 week (T1), exercise 2 weeks (T2), exercise 3 weeks (T3), exercise 4 weeks (T4), exercise 5 weeks (T5) and control group before and after exercise (C0 C5). The exercise group was trained with 6% body weight. After 48 hours of training, the samples were selected for testing. The changes of protein and gene mRNA were detected by Western blot- RT-PCR and other techniques, such as Starn PKA, pCREBEBSF-1 and so on. Results: the level of lactic acid in group A and group B was significantly higher than that in group C (P0.01) after exercise in group A and group B (P0.01), and there was a significant difference between group A and group B (P0.01). CK and BU were significantly increased in exercise intervention group (p0.01), but the expression of StAR mRNA in group B was significantly lower than that in group C (p0.05). In formal experiment 1, the serum testosterone level in the exercise intervention group decreased slowly in the first three weeks, then increased, and the serum testosterone level in the T5 group decreased by 70% (p0.01) .LH in comparison with the C0 group. In the fifth week, the LH level decreased more significantly than that of C0, and the difference was significant (p0.01). The level of 3min lactic acid in the exercise intervention group increased significantly after training. The trend of change was: the first three weeks increased greatly (p0.01), the latter two weeks maintained a higher level and slightly increased (p0.05) .SOD activity continued to decrease. In the fifth week, the activity of SOD decreased to 77% (p0.01). The content of SOD fluctuated slightly in the first three weeks, and increased significantly in the fourth and fifth weeks, which was significantly different from that in the C0 group (p0.01) .The expression of PKAP-CREBSF-1 protein in testis of rats remained stable in the first three weeks. The expression of StAR mRNA in the exercise intervention group did not change significantly in the first three weeks, but the expression of mRNA in the T5 group was significantly different from that in the quiet group (p0.05). The SF-1 mRNA increased slightly in the first week compared with that in the C _ 0 group, and then gradually decreased to the fifth week (p _ (0.01). Conclusion the level of peroxidation in testicular tissue was increased after 1 and 5 weeks of submaximum intensity exercise, and the serum testosterone level was increased. The decrease of StAR and its related regulatory factor PKApCREBSF-1 in Leydig cells of testis. 2StAR-related regulatory factors were inhibited, which led to the decrease of StAR mRNA expression and the restriction of cholesterol transport, which may be the cause of accelerating the decrease of testosterone.
【学位授予单位】：上海体育学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7

【相似文献】