8周间歇运动激活LIF-LIFR-STAT3信号和诱导骨骼肌细胞增殖

发布时间：2018-12-31 16:20

【摘要】：目的:探讨间歇运动对大鼠骨骼肌细胞增殖和白血病抑制因子(Leukemia inhibitory factor,LIF)及其下游信号分子表征的影响。方法:3月龄雄性SD大鼠,随机分为安静对照组(C)、间歇运动组(CE),每组15只。运动组采用小动物跑台运动,运动方式为先以10 m/min×10 min,再以25 m/min×7 min和15m/min×3 min,依次交替,60 min/d,5 d/周×8周。运动结束后次日,分离切取大鼠腓肠肌组织,称重。组织学方法测定腓肠肌细胞横截面积(cross-section area,CSA);Western blot检测腓肠肌LIF及其受体(Leukemia inhibitory factor receptor,LIFR)、信号转导与转录激活因子(signal transducer and activator of transcription,STAT3)及其磷酸化水平(p-STAT3)和增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)蛋白表达;免疫荧光化学观察分析腓肠肌LIF、LIFR、PCNA和细胞增殖抗原标记物(ki67)表达;RT-q PCR检测腓肠肌LIF/LIFR mRNA水平。结果:与C组比较,CE组大鼠腓肠肌重量和细胞CSA增大(P0.01),LIF/LIFR基因和蛋白表达均显著增加(P0.01,P0.05),STAT3磷酸化水平显著升高(P0.01),PCNA和ki67蛋白表达显著增加(P0.01)。结论:8周间歇运动可促进正常大鼠骨骼肌细胞肥大,激活LIF-LIFR-STAT3信号通路,诱导骨骼肌细胞增殖。
[Abstract]:Aim: to investigate the effects of intermittent exercise on the proliferation of skeletal muscle cells and the characterization of leukemia inhibitory factor (Leukemia inhibitory factor,LIF) and its downstream signal molecules in rats. Methods: three month old male SD rats were randomly divided into quiet control group (C), intermittent exercise group) and (CE), group (15 rats in each group). In the exercise group, 10 m/min 脳 10 min, followed by 25 m/min 脳 7 min and 15m/min 脳 3 min, 60 min/d,5 d / week 脳 8 weeks. The next day after exercise, the gastrocnemius muscle tissue was removed and weighed. The cross sectional area (cross-section area,CSA) of gastrocnemius muscle cells was measured by histological method. The expression of LIF and its receptor (Leukemia inhibitory factor receptor,LIFR, signal transduction and transcription activator (signal transducer and activator of transcription,STAT3 (signal transducer and activator of transcription,STAT3) and its phosphorylation level (p-STAT3) and proliferating cell nuclear antigen (proliferating cell nuclear antigen,PCNA) in gastrocnemius muscle were detected by Western blot. The expression of LIF,LIFR,PCNA and cell proliferation antigen marker (ki67) in gastrocnemius was detected by immunofluorescence, and the LIF/LIFR mRNA level in gastrocnemius was detected by RT-q PCR. Results: compared with group C, the weight of gastrocnemius muscle and the expression of LIF/LIFR gene and protein in gastrocnemius muscle increased significantly (P0.01), the expression of LIF/LIFR gene and protein increased significantly (P0.01), and the level of STAT3 phosphorylation increased significantly (P0.01) in CE group. The expression of PCNA and ki67 protein increased significantly (P0.01). Conclusion: 8 weeks intermittent exercise can promote the hypertrophy of skeletal muscle cells, activate the LIF-LIFR-STAT3 signal pathway and induce the proliferation of skeletal muscle cells.
【作者单位】：陕西师范大学体育学院暨运动生物学研究所;DepartmentofBiochemistryandMolecularBiologyUniversityofMarylandSchoolofMedicineBaltimore21202;
【基金】：国家自然科学基金资助项目(编号:31371199,31171141) 陕西师范大学“211工程”重点建设学科——运动生物学学科建设项目(编号:2014-12)
【分类号】：G804.2

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