长期递增负荷运动对大鼠股骨OPG和RANKL蛋白表达的影响
发布时间：2019-04-26 09:04
【摘要】：目的:观察长期递增负荷运动对大鼠股骨OPG、RANKL蛋白表达以及BMD的影响效应。方法:130只3月龄SD雌性大鼠,适应性训练后随机分为运动组和对照组。运动组分别进行为期4、9、11、13、15、17 W的递增负荷跑台运动(E_4、E_9、E_(11)、E_(13)、E_(15)、E_(17)),并设立同期对照组(C_4、C_9、C_(11)、C_(13)、C_(15)、C_(17))和基础对照组(BC),每组10只。于末次运动后12~24 h,以DXA测定大鼠全身BMD,用Western Blot方法测定股骨OPG和RANKL的蛋白表达。结果:在17 W递增负荷跑台运动过程中,运动组大鼠OPG表达及OPG/RANKL比值呈现先升高趋势,于第9 W达到峰值,随后呈下降的趋势,于17 W降至最低(E_(17)vs.C17,P0.05,ES=2.66;P0.05,ES=2.65)。运动组可溶性RANKL(sRANKL)、膜结合型RANKL(mRANKL)及总RANKL表达呈现先下降趋势,于第9 W或11 W时降至最低,随后呈逐渐升高的趋势。在17 W运动过程中,运动组大鼠全身BMD先表现为增加趋势,于第9W达到峰值(E_9vs.C_9,P0.05,ES=0.85),随后呈下降趋势,于17 W降至最低(E_(17)vs.C_(17),P0.01,效果量=1.49)。结论:长期递增负荷运动过程中,前9 W适量运动可上调OPG表达、下调RANKL表达,抑制骨吸收,促进BMD增加;后期过量运动则抑制OPG表达、促进RANKL表达,骨吸收作用增强,导致BMD下降。
[Abstract]:Aim: to observe the effect of long-term incremental exercise on the expression of OPG,RANKL protein and BMD in rat femur. Methods: 130 3-month-old female SD rats were randomly divided into exercise group and control group after adaptive training. The exercise group was divided into 4, 9, 11, 13, 15, 17 W incremental load races (E / 4, E _ (9), E _ (11), E _ (13), E _ (15), E _ (17),) and the control group (C ~ (4), C ~ (9), C _ (17), C _ (11), E _ (13), E _ (15), E _ (17),). C _ (11), C _ (13), C _ (15), C _ (17) and basal control group (BC),) were 10 rats in each group. At 12 h after the last exercise, the protein expression of OPG and RANKL in femur was measured by Western Blot method in the whole body of BMD, of rats. DXA was used to measure the expression of OPG and RANKL in the whole body of rats. Results: the expression of OPG and the ratio of OPG/RANKL in the exercise group increased at first, reached the peak at the 9th week, then decreased to the lowest level at 17 W (E17 vs.C17,). P0.05, ESL 2.66; (P 0.05, ESL 2.65). In the exercise group, the expression of soluble RANKL (sRANKL), membrane-bound RANKL (mRANKL) and total RANKL decreased first, decreased to the lowest at 9 W or 11 W, and then increased gradually. During 17 W exercise, the whole body BMD of rats in the exercise group showed an increasing trend at first and reached a peak at the 9th week (E = 9 vs. C = 9, P 0.05, es = 0.85), and then showed a decreasing trend, while the whole body BMD of the exercise group showed an increasing trend at first and reached a peak at the 9th week. It decreased to the lowest level at 17 W (E17 vs.C_ (17), P0.01, effect = 1.49). Conclusion: during long-term incremental exercise, moderate exercise in the first 9 W can up-regulate the expression of OPG, down-regulate the expression of RANKL, inhibit bone resorption and promote the increase of BMD. Excessive exercise inhibited the expression of OPG, promoted the expression of RANKL and increased the bone resorption, which resulted in the decrease of BMD.
【作者单位】： 北京体育大学运动人体科学学院;首都体育学院运动与健康学院;山东体育学院体育科学研究院;
【基金】：北京市教委科研基地科技创新平台项目“运动健骨机制研究”(PXM2012-014206-000037) 山东省高等学校优势学科人才团队培育计划
【分类号】：G804.2
本文编号：2465940
[Abstract]:Aim: to observe the effect of long-term incremental exercise on the expression of OPG,RANKL protein and BMD in rat femur. Methods: 130 3-month-old female SD rats were randomly divided into exercise group and control group after adaptive training. The exercise group was divided into 4, 9, 11, 13, 15, 17 W incremental load races (E / 4, E _ (9), E _ (11), E _ (13), E _ (15), E _ (17),) and the control group (C ~ (4), C ~ (9), C _ (17), C _ (11), E _ (13), E _ (15), E _ (17),). C _ (11), C _ (13), C _ (15), C _ (17) and basal control group (BC),) were 10 rats in each group. At 12 h after the last exercise, the protein expression of OPG and RANKL in femur was measured by Western Blot method in the whole body of BMD, of rats. DXA was used to measure the expression of OPG and RANKL in the whole body of rats. Results: the expression of OPG and the ratio of OPG/RANKL in the exercise group increased at first, reached the peak at the 9th week, then decreased to the lowest level at 17 W (E17 vs.C17,). P0.05, ESL 2.66; (P 0.05, ESL 2.65). In the exercise group, the expression of soluble RANKL (sRANKL), membrane-bound RANKL (mRANKL) and total RANKL decreased first, decreased to the lowest at 9 W or 11 W, and then increased gradually. During 17 W exercise, the whole body BMD of rats in the exercise group showed an increasing trend at first and reached a peak at the 9th week (E = 9 vs. C = 9, P 0.05, es = 0.85), and then showed a decreasing trend, while the whole body BMD of the exercise group showed an increasing trend at first and reached a peak at the 9th week. It decreased to the lowest level at 17 W (E17 vs.C_ (17), P0.01, effect = 1.49). Conclusion: during long-term incremental exercise, moderate exercise in the first 9 W can up-regulate the expression of OPG, down-regulate the expression of RANKL, inhibit bone resorption and promote the increase of BMD. Excessive exercise inhibited the expression of OPG, promoted the expression of RANKL and increased the bone resorption, which resulted in the decrease of BMD.
【作者单位】： 北京体育大学运动人体科学学院;首都体育学院运动与健康学院;山东体育学院体育科学研究院;
【基金】：北京市教委科研基地科技创新平台项目“运动健骨机制研究”(PXM2012-014206-000037) 山东省高等学校优势学科人才团队培育计划
【分类号】：G804.2
【相似文献】
相关会议论文 前1条
1 邹军;董洁琼;李丽辉;秦淑娟;;运动对去卵巢骨质疏松大鼠OPG、RANKL表达的影响[A];第九届全国体育科学大会论文摘要汇编(3)[C];2011年
相关硕士学位论文 前1条
1 李盛村;NO、OPG/RANKL在大鼠运动性骨损伤发生过程中的表达及作用研究[D];苏州大学;2013年
，本文编号：2465940
本文链接：https://www.wllwen.com/jiaoyulunwen/tylw/2465940.html
