典型纳米材料对甲烷化反硝化厌氧氨氧化系统的影响

发布时间：2018-01-25 06:22

本文关键词： FFOMNs ZnO MWNTs SMDA 系统　出处：《安徽大学》2017年硕士论文　论文类型：学位论文

【摘要】：甲烷化反硝化厌氧氨氧化(SMDA)系统具有产甲烷同时脱除无机氮(NH4+-N、NO2--N和NO3--N)、有机氮和有机碳的功能。本文通过在UASB反应器中构建SMDA系统,探讨了不同纳米材料(FFOMNs、ZnO和MWNTs)对SMDA系统运行效能、颗粒污泥特性、微生物菌群结构的影响。主要研究结论如下:(1)UASB反应器经500天运行,完成了 SMDA系统构建,稳定运行和重新启动三个过程。在UASB稳定运行阶段,U1(U2)反应器中,COD、NH4+-N、NO2--N、N03--N 的去除效率分别为 75%(70%)、48%(44%)、99.99%(99.99%)、99.99%(99.99%);N2和CH4所占比例分别约为75%和25%。(2)SMDA系统主要由产甲烷菌群,反硝化菌群,厌氧氨氧化菌群、发酵细菌四种关键营养菌群组成。四种关键营养菌群可在单一 UASB反应器中共存,并形成一种具有多层结构的厌氧颗粒污泥(产甲烷菌在颗粒污泥内部,反硝化细菌和厌氧氨氧化菌在颗粒污泥外层)。(3)300mg/L以内的FFOMNs对SMDA系统基本没有影响。400mg/L以内ZnO纳米颗粒对甲烷化过程具有抑制作用,随浓度的增加,抑制作用越强,但对厌氧氨氧化过程有一定促进作用。100、200、和400 mg/L ZnO纳米颗粒对反硝化过程分别具有促进作用、无明显影响和抑制作用。MWNTs对于SMDA系统的影响表现为:低浓度(10 mg/L)起促进作用,中浓度(300 mg/L)不起作用,高浓度(1000mg/L)起抑制作用。(4)ZnO和MWNTs能刺激颗粒污泥分泌EPS,从而影响颗粒污泥内部结构,EPS的分泌量和纳米颗粒浓度成正相关。纳米材料使SMDA系统中关键功能菌丰度发生明显改变;随纳米材料浓度的增加,产甲烷菌所占比例逐渐增加,而反硝化菌和厌氧氨氧化菌所占比例逐渐减少。
[Abstract]:The methanation and denitrification anaerobic ammonia oxidation (SMDAA) system is characterized by the simultaneous removal of inorganic nitrogen, NH _ 4-N, no _ 2-N and no _ 3-N). The function of organic nitrogen and organic carbon. In this paper, SMDA system was constructed in UASB reactor to discuss the different nano-materials FFOMNs. The effects of ZnO and MWNTs on the performance of SMDA system, the characteristics of granular sludge and the structure of microbial flora were studied. The three processes of SMDA system construction, stable operation and restart were completed. In the UASB stable operation stage, the COD NH _ 4-N no _ 2-N was added to the reactor. The removal efficiency of N03-N is 75%, 70% and 444%, respectively, about 99.99% and 99.9999, and 99.9999 and 99.99%, and 99.99%, 99.99%, 99.99% and 99.99%, respectively. The proportions of N2 and CH4 were about 75% and 25 respectively, which were mainly composed of methanogenic bacteria, denitrifying bacteria and anaerobic ammonia-oxidizing bacteria. The four key nutrient bacteria can coexist in a single UASB reactor and form a multi-layer anaerobic granular sludge (methanogenic bacteria in the granular sludge). Denitrifying bacteria and anaerobic ammonia-oxidizing bacteria in the outer layer of granular sludge. FFOMNs within 300mg / L had no effect on the SMDA system. The ZnO nanoparticles within 400mg / L could inhibit the methanation process. With the increase of concentration, the inhibitory effect is stronger, but it can promote the process of anaerobic ammonia oxidation. And 400 mg/L ZnO nanoparticles promoted the denitrification process, respectively. The effect of MWNTs on SMDA system was as follows: low concentration of 10 mg / L) could promote it, while the medium concentration of 300 mg / L did not. High concentration of 1 000 mg / L could inhibit the secretion of EPSs from granular sludge, thus affecting the internal structure of granular sludge. There was a positive correlation between the secretion of EPS and the concentration of nanoparticles. The abundance of critical functional bacteria in SMDA system was obviously changed by nano-materials. With the increase of nano-material concentration, the proportion of methanogenic bacteria gradually increased, while the proportion of denitrifying bacteria and anaerobic ammonia-oxidizing bacteria gradually decreased.
【学位授予单位】：安徽大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：X703;TB383.1

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