普鲁士蓝纳米粒子的光热毒性研究

发布时间：2018-04-19 00:35

本文选题：普鲁士蓝 + 纳米粒子　；参考：《上海师范大学》2015年硕士论文

【摘要】：在科学技术高速发展的今天,学科交叉已经成为一大趋势,其中纳米材料在医学领域的应用尤为瞩目。纳米材料因其独特的生物医学优势有望解决传统医学方法的生物医学问题,引领现代医学诊断和治疗方法的新变革。然而当前多数纳米材料的研究不够深入,其在人体内的生物安全性得不到保障,且很多材料制备复杂、价格昂贵,严重限制了其进一步发展。因此,纳米毒理学应运而生。它与传统毒理学相互渗透并逐渐发展,融入纳米技术、材料科学、生物学、医学、毒理学、药理学等方面的基础知识,对纳米技术的安全性以及人类社会至关重要。同时,纳米材料光热治疗日渐兴起,经过激光照射后,利用纳米光热剂表面等离子体共振产热,产生高温杀死肿瘤细胞,可以达到治疗癌症的目的。普鲁士蓝是一种古老的试剂,其制备简单、价格便宜、光热性能优异。最为重要的是普鲁士蓝长期的临床用药验证了其在人体内可靠的生物安全性和代谢途径。正是由于这些优势,普鲁士蓝的纳米材料一旦被深入应用于医学研究,将有潜力解决其它多数纳米材料生物安全性得不到保障且价格昂贵的医学难题,其研究和应用价值巨大。作为一种优良光热试剂因其良好的光热性能和极高的生物相容性被用于生物医学领域,然而其光热致死癌细胞的毒性机理却鲜有人问津。本论文针对普鲁士蓝纳米粒子(PB NPs)的光热性质、光热稳定性、细胞毒性、细胞光热毒性进行了探究,分析了对比了四种细胞He La、Chang liver、4T1、L929在不同浓度PB纳米粒子浓度和不同激光功率密度下的细胞死亡方式,并进一步探讨了影响这些细胞光热产生致死性的毒性的因素。
[Abstract]:With the rapid development of science and technology, interdisciplinary has become a major trend, among which the application of nano-materials in the field of medicine is particularly attractive.Nanomaterials, due to their unique biomedical advantages, are expected to solve the biomedical problems of traditional medical methods and lead to new changes in modern medical diagnosis and treatment.However, most of the current research on nanomaterials is not deep enough, their biological safety in human body can not be guaranteed, and many materials are complex and expensive, which seriously limits their further development.Therefore, nanometer toxicology arises at the historic moment.It permeates with traditional toxicology and develops gradually. It is very important for the safety of nanotechnology and human society to integrate the basic knowledge of nanotechnology, material science, biology, medicine, toxicology, pharmacology and so on.At the same time, photothermal therapy of nanomaterials is emerging day by day. After laser irradiation, the surface plasmon resonance (SPR) of nano-photothermal agent is used to produce heat and kill tumor cells at high temperature, which can be used to treat cancer.Prussian blue is an ancient reagent, it is simple to prepare, cheap, excellent photothermal properties.The most important thing is that Prussian blue has proven its biological safety and metabolic pathway in human body.Because of these advantages, Prussian Blue nanomaterials, once used in medical research, will have the potential to solve the other nanomaterials biological safety can not be guaranteed and expensive medical problems, its research and application is of great value.As a kind of excellent photothermal reagent, it has been used in biomedical field because of its good photothermal properties and high biocompatibility. However, the toxic mechanism of photothermal lethal cancer cells is rarely studied.In this paper, the photothermal properties, photothermal stability, cytotoxicity and photothermal toxicity of Prussian blue nanoparticles (PB NPs) were investigated.In this paper, the cell death patterns of four kinds of He-La-Chang Chang livertio 4T _ (1) L _ (929) cells under different concentrations of PB nanoparticles and different laser power densities were analyzed and compared, and the factors influencing the lethal toxicity of these cells were discussed.
【学位授予单位】：上海师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TB383.1

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