多巴胺铜簇用于检测四环素类药物以及番茄汁碳点荧光探针的建立

发布时间：2018-04-26 13:08

本文选题：铜纳米团簇 + 碳点　；参考：《西南大学》2017年硕士论文

【摘要】：本论文主要以荧光探针为研究对象,然后分别以荧光金属纳米团簇和荧光碳点为信号基础,制备了一种以多巴胺为模板合成的铜纳米团簇和一种以番茄汁为碳源合成的碳点,分别考察两种荧光纳米传感材料的荧光性能、结构以及稳定性等。然后用多巴胺铜纳米团簇建立了一种用于四环素类药物检测的荧光分析方法,实现目标检测物的特异性敏感分析,并考察了其在实际样品中的分析应用;另外我们还应用番茄汁碳点进行了生物成像。本论文主要研究内容如下:(1)以多巴胺为模板,在水溶液中,以硼氢化钠为还原剂还原硫酸铜,一步合成了具有荧光的铜纳米团簇(Copper nanoclusters,Cu NCs),通过紫外-可见吸收光谱、红外光谱、荧光光谱、高分辨透射电子显微镜(HR-TEM)等对其表征。所制得的Cu NCs的最佳激发和发射波长分别为425 nm和485 nm,且能够发出明亮的蓝绿色荧光。该Cu NCs直径为2-3 nm,尺寸比较均一,且没有团聚现象。当Cu NCs溶液中加入一定量的四环素类药物(TCs)时,Cu NCs表面基团和四环素类药物表面之间反应电位降低,导致Cu NCs的荧光强度降低。因此,利用所合成的铜簇对四环素类药物实现了灵敏的检测。首先我们用此种方法对四环素(TC)检测进行了考查,其荧光强度的减小与TC浓度在3.6×10~(-6) mol·L~(-1)到1.0×10~(-3) mol·L~(-1)范围内呈线性关系,其检测限为(3σ)9.2×10~(-7)mol·L~(-1)。然后分别又对金霉素和多西环素进行了检测,虽然也有响应,但是灵敏度不高,检测范围较窄。最后,将本文所建立的方法应用于牛奶中TC的检测分析,进一步证实了此方法的实用性。此外,我们还将此铜簇进行了荧光油墨以及加密防伪方面的应用。(2)以番茄汁作为碳源,运用了一种绿色环保、创新的一步合成方法制备得了碳点(CDs)。一方面合成碳点所用的碳源物质绿色环保,另一方面所合成的碳点也具有光漂白性好、高荧光量子产率、稳定性好和毒性低等优点。CDs高分辨透射电子显微镜成像(HR-TEM)结果显示其平均粒径为3 nm,且分布均匀。随后红外光谱结果表明在CDs表面含有-NH,-OH,-COOH等功能基团。同时用碳点进行了细胞毒性实验,且利用碳点优异的荧光性能进行了生物成像。上述结果对我们深入认识碳点的性能,以及对进一步将其应用于传感分析中都具有重要价值。
[Abstract]:In this thesis, a kind of copper nanoclusters synthesized by dopamine template and a carbon point synthesized by tomato juice as carbon source were prepared on the basis of fluorescent metal nanoclusters and fluorescent carbon spots, respectively. The fluorescence properties, structure and stability of two kinds of fluorescent nanosensors were investigated. Then a fluorescence analysis method for tetracycline drug detection was established by using dopamine copper nanoclusters to realize the specificity and sensitivity analysis of the target and its application in practical samples was investigated. In addition, we also used tomato juice carbon spots for biological imaging. The main contents of this thesis are as follows: (1) Copper nanoclusters copper nanoclusters with fluorescence were synthesized by using dopamine as template and sodium borohydride as reductant to reduce copper sulfate in aqueous solution. Fluorescence spectra and high resolution transmission electron microscopy (HR-TEM) were used to characterize them. The optimum excitation and emission wavelengths of Cu NCs are 425nm and 485nm, respectively, and can emit bright blue-green fluorescence. The diameter of Cu NCs is 2-3 nm, the size is uniform and there is no agglomeration. When a certain amount of tetracycline was added to Cu NCs solution, the reaction potential between the surface group of Cu NCs and the surface of tetracycline was decreased, which resulted in the decrease of fluorescence intensity of Cu NCs. Therefore, the synthesized copper clusters were used to detect tetracycline drugs sensitively. First of all, we used this method to detect tetracycline (TCC). The decrease of fluorescence intensity is linear with TC concentration in the range of 3.6 脳 10 ~ (-6) mol / L ~ (-1) to 1.0 脳 10 ~ (-3) mol / L ~ (-1), and the detection limit is 3 蟽 ~ 9.2 脳 10~(-7)mol ~ (-1) 路L ~ (-1). Then chlortetracycline and doxycycline were detected respectively. Although there was a response, the sensitivity was not high and the detection range was narrow. Finally, the proposed method is applied to the detection and analysis of TC in milk, which further proves the practicability of the method. In addition, the copper cluster was used in fluorescent ink and encryption anti-counterfeiting applications. The tomato juice was used as carbon source, and a green and innovative one-step synthesis method was used to prepare the carbon dot CDsN. On the one hand, the carbon sources used in the synthesis of carbon dots are green and environmentally friendly; on the other hand, the carbon dots synthesized also have good photobleaching properties and high fluorescence quantum yields. The results of HR-TEM imaging show that the average particle size of CDs is 3 nm and its distribution is uniform. The infrared spectra show that there are some functional groups such as -NH _ (-) -OH _ (-COOH) on the surface of CDs. At the same time, the cytotoxicity test was carried out with carbon spots, and the excellent fluorescence properties of carbon spots were used for biologic imaging. These results are of great value for us to understand the properties of carbon points and to further apply them to sensor analysis.
【学位授予单位】：西南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3;TB383.1

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