聚乳酸纳米粒子的制备及其作为免疫佐剂的应用研究

发布时间：2018-05-02 16:41

本文选题：聚乳酸纳米粒子 + 免疫佐剂　；参考：《北京化工大学》2015年硕士论文

【摘要】：纳米技术是一种很有发展潜力的新型技术。纳米材料,以其粒径小、比表面积大、性能优越等特点,具有广泛的应用前景。生物可降解的高分子聚合物制备成的纳米粒子在生物医药领域具有研究和应用潜力。本论文主要研究聚乳酸纳米粒子的制备方法并进行工艺优化,将制备的聚乳酸纳米粒子应用于免疫佐剂的研究。取得的具体研究结果如下：1、研究纳米沉淀法制备聚乳酸纳米粒子,采用响应面优化法对制备工艺进行优化,最优条件制备得到的聚乳酸纳米粒子粒径为109.1 nm,PDI为0.085,粒径均一,分散性良好；2、研究自乳化-溶剂挥发法制备聚乳酸纳米粒子,对其进行工艺优化。采用阴离子表面活性剂十二烷基硫酸钠(SDS)制备表面带负电的聚乳酸纳米粒子,优化工艺制备的聚乳酸纳米粒子的粒径为126.8 nm, PDI值为0.129, Zeta电位为-53.7 mV,粒径均一,分散性良好；采用阳离子表面活性剂双十二烷基二甲基溴化铵(DDAB),制备表面带正电的聚乳酸纳米粒子,优化工艺制备得到纳米粒子的粒径为101.7 nm, PDI值为0.078,Zeta电位为+37 mV,粒径均一,分散性良好；3、将带正电的聚乳酸纳米粒子作为免疫佐剂开展免疫效果研究,考察了100 nm、300 nm和1μm三种粒径的聚乳酸颗粒,测定不同粒径免疫佐剂效果,结果表明100 nm聚乳酸纳米粒子能诱导更高水平的血清特异性抗体、细胞因子等表达,免疫效果更好；4、研究了表面带负电的聚乳酸纳米粒子的溶胀制孔,分别采用二次乳液溶胀法、载气溶胀法和动态溶胀法三种方法进行纳米粒子的制孔研究,对三种方法的实验装置、实验条件进行优化,成功地在纳米粒子内部制出孔道。
[Abstract]:Nanotechnology is a new technology with great potential for development. Nanomaterials, with their small particle size, large specific surface area, superior performance and other characteristics, have a wide range of application prospects. The nanoparticles prepared by biodegradable polymer have the potential of research and application in the field of biomedicine. In this paper, the preparation method of polylactic acid nanoparticles was studied and the preparation process was optimized. The prepared polylactic acid nanoparticles were applied to the study of immune adjuvant. The specific results obtained are as follows: 1. The preparation of polylactic acid nanoparticles by nano-precipitation method was studied. The preparation process was optimized by response surface optimization method. The particle size of polylactic acid nanoparticles prepared under the optimum conditions was 0.085 and 0.085, and the particle size was uniform. Poly (lactic acid) nanoparticles were prepared by self-emulsifying-solvent volatilization method. Polylactic acid nanoparticles with negative charge were prepared by anionic surfactant sodium dodecyl sulfate. The particle size, PDI value and Zeta potential were 126.8 nm, 0.129 and -53.7 MV, respectively. The polylactic acid nanoparticles with positive charge on the surface were prepared by using the cationic surfactant didodecyl dimethylammonium bromide (DDABN). The particle size of the nanoparticles was 101.7 nm, the PDI value was 0.078% Zeta potential was 37 MV, and the particle size was uniform. The immunological effect of polylactic acid nanoparticles with positive charge as immune adjuvant was studied. Three kinds of polylactic acid particles (100nm ~ 300nm and 1 渭 m) were investigated. The results showed that 100nm polylactic acid nanoparticles could induce a higher level of serum specific antibodies, cytokines and other expressions, and the immune effect was better. The swelling of polylactic acid nanoparticles with negative charge on the surface was studied. The pore preparation of nanoparticles was studied by means of secondary emulsion swelling method, gas carrying swelling method and dynamic swelling method respectively. The experimental equipment and experimental conditions of the three methods were optimized, and the pore channels were successfully made in the nanoparticles.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：O633.14;TB383.1

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