基于多孔碳材料的酶生物燃料电池酶电极的研究

发布时间：2018-01-05 09:04

  本文关键词：基于多孔碳材料的酶生物燃料电池酶电极的研究　出处：《湖南大学》2016年硕士论文　论文类型：学位论文

  更多相关文章： 酶生物燃料电池 多孔碳材料 二茂铁 中间体 葡萄糖氧化酶

【摘要】：酶生物燃料电池(EBFC)是一类以生物质燃料为原料,生物酶为催化剂将化学能转化为电能的有效装置,与传统电池相比,具有能量转化率高、生物相容性好、原料来源广等优势,是一种真正意义上的绿色电池,有望解决环境与能源的危机。目前需要解决的问题是让酶高效固定在电极上且实现电子的有效传递。本文以三维多孔泡沫碳(3 D-PCF)或超短掺氮碳纳管(N-STCs)为电极载体材料,进行表面修饰以此来固定酶和中间体,并通过多种表征方法系统的研究了酶电极和燃料电池的性能。本文具体研究内容如下：(1)以三维多孔泡沫碳(3D-PCF)为电极载体材料,通过氨基二茂铁的重氮化实现了中间体在3D-PCF上的固定,得到Fc@3D-PCF,然后利用戊二醛将壳聚糖(CS)和葡萄糖氧化酶(GOD)共价交联到Fc@3D-PCF上,制备GOD-CS/Fc@3D-PCF电极材料。构建以GOD-CS/Fc@3D-PCF/GC为阳极和E-TEKPt/C电极为阴极的葡萄糖/O2 EBFC。所构建的酶生物燃料电池的开路电位(Voc)和短路电流(Isc)分别为0.77 V和0.38 mA cm-2,在0.50 V时,获取最大的功率输出密度为64.3μW cm-2。(2)基于已制备的固定了氨基二茂铁的材料Fc@3D-PCF,然后利用醛基二茂铁的醛基和GOD的氨基通过席夫碱共价链接得到Fc-GOD,再通过GA交联Fc-GOD附着在Fc@3D-PCF上制得GOD-Fc/Fc@3D-PCF电极材料,构建了以GOD-CS/Fc@3D-PCF/GC为阳极和E-TEKPt/C电极为阴极的葡萄糖/O2 EBFC。所构建的酶生物燃料电池的开路电位(Voc)和短路电流(Isc)分别为0.88 V和0.58 mAcm-2,在0.40 V时,获取最大的功率输出密度为116.4μW cm-2,较之前固定单-中间体各项性能有明显的提升(3)利用自制掺氮碳纳米管(N-STC)为载体材料,通过负载纳米金并共同固定中间体和葡萄萄糖氧化酶的方法,制备了EBFC阳极GOD/Fc-CYS-Au/N-STC,并构建了以GOD/Fc-CYS-Au/N-STC电极为阳极和E-TEK Pt/C电极为阴极的葡萄糖/O2EBFC,所构建的酶生物燃料电池的开路电位(Voc)和短路电流(Isc)分别为0.71 V和01.78 mA cm-2,在0.21 V时,获取最大的功率输出密度为83.6μW cm-2。
[Abstract]:Enzyme biofuel cell (EBFC) is an effective device which uses biomass fuel as raw material and enzyme as catalyst to convert chemical energy into electric energy. Because of its advantages of good biocompatibility and wide sources of raw materials, it is a kind of real green battery. It is expected to solve the environmental and energy crisis. At present, the problem that needs to be solved is that the enzyme can be efficiently immobilized on the electrode and the effective transfer of electrons can be realized. In this paper, the three-dimensional porous carbon foam 3D-PCF is used to solve the problem. Or ultrashort nitrogen-doped carbon nanotubes (N-STCs) are used as electrode carrier materials. To immobilize enzymes and intermediates by surface modification. The performance of enzyme electrode and fuel cell was systematically studied by various characterization methods. The main contents of this paper are as follows: 1) Three-dimensional porous carbon foam 3D-PCF) was used as electrode carrier. The intermediate was immobilized on 3D-PCF by diazotization of ferrocene and Fc@3D-PCF was obtained. Then glutaraldehyde was used to cross-link chitosan (CSS) and glucose oxidase (GODs) to Fc@3D-PCF. Preparation of GOD-CS/Fc@3D-PCF electrode Materials. Fabrication of glucose / O _ 2 with GOD-CS/Fc@3D-PCF/GC as anode and E-TEKPt / C electrode as cathode. Open-circuit potential of enzyme biofuel cell constructed by EBFC. Vocabulary and short circuit current were 0.77V and 0.38mA cm-2, respectively. At 0.50 V, the maximum power output density of 64.3 渭 W cm-2.2) was obtained based on the immobilized material Fc@3D-PCF. Then Fc-GOD was obtained by covalent linking of aldehyde group of aldehyde ferrocene and amino group of GOD by Schiff base. Then the GOD-Fc/Fc@3D-PCF electrode material was prepared by GA crosslinking Fc-GOD attached to Fc@3D-PCF. Using GOD-CS/Fc@3D-PCF/GC as anode and E-TEKPT / C electrode as cathode, glucose / O _ 2 / O _ 2 was constructed. Open-circuit potential of enzyme biofuel cell constructed by EBFC. Vocabulary and short circuit current were 0.88V and 0.58 mAcm-2, respectively. At 0.40 V, the maximum power output density is 116.4 渭 W cm-2. Compared with the previous fixed mono-intermediates, the properties of the mono-intermediates were improved obviously. The N-STC / NT-doped carbon nanotubes (NCNTs) were used as the support materials. EBFC anode GOD/Fc-CYS-Au/N-STC was prepared by loading nano-gold and fixing intermediate and grape glucose oxidase. Glucose / O2 EBFC with GOD/Fc-CYS-Au/N-STC electrode as anode and E-TEK Pt/C electrode as cathode was constructed. The open circuit potential (Vocg) and short circuit current (Iscc) of the constructed enzyme biofuel cell were 0.71 V and 0.78 Ma / cm ~ (-2), respectively, at 0.21 V. The maximum power output density is 83.6 渭 W cm-2.
【学位授予单位】：湖南大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TQ127.11;TM911.4
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本文编号：1382432