植物组培的高效智能LED光照系统设计

发布时间：2018-06-26 23:58

本文选题：智能光照系统 + 光合有效量子通量密度　；参考：《中国计量学院》2014年硕士论文

【摘要】：光是影响植物组织培养的重要因素。针对传统荧光灯补光方式存在的能耗高、只有简单的开关功能等问题，本课题研制了一种高效智能LED光照系统，对于降低组培成本，解决组培室光照控制困难等问题具有实际应用意义，具体研究内容如下：（1）系统硬件的实现：包括通信模块、终端控制器、光照度检测模块、LED补光模块。根据设计要求，构建ZigBee无线网络，解决组培室连线众多，管理繁杂的问题；设计基于STM8S单片机和REX3U无线模块的控制系统，实现光强、光质可调；选用硅光电池为光照度检测模块的光电探测器，用于监测光环境变化；LED补光模块由LED光源和恒流驱动电路组成。（2）系统软件的开发：包括通信协议、上位机人机界面、下位机执行程序。根据ZigBee无线模块底层要求制定通信传输协议，使用C#语言编写上位机操作界面整体架构和功能模块，方便用户对组培室管理，使用C语言编写下位机各模块程序，完成信号接收、解析和处理。（3）基于光度学原理建立光照度与光合有效量子通量密度关系，并用实验验证了其正确性。结果表明，红蓝光的光照度与光合有效量子通量密度比值k，其实验值与理论值相对误差分别为3.86%、2.66%，光合有效量子通量密度控制误差在5%之内。系统可提供最大光照强度为90μmol m-2s1，在光强65μmol m-2s1下，，最大红蓝光强比12：1。（4）应用研制的光照系统，在不同光质下对铁皮石斛组培苗培育试验。结果表明，光质RB5:1时对铁皮石斛不定芽数、苗高、节数、节间长度促进作用最明显，不同光质对干物质和叶绿素积累影响差别不大。
[Abstract]:Light is an important factor affecting plant tissue culture. Aiming at the problems of high energy consumption and simple switching function in the traditional fluorescent lamp compensation mode, an efficient and intelligent LED lighting system is developed in this paper, which can reduce the cost of tissue culture. It is of practical significance to solve the difficulty of illumination control in tissue culture room. The specific research contents are as follows: (1) the hardware realization of the system includes communication module, terminal controller, light intensity detection module and LED light compensation module. According to the design requirements, the ZigBee wireless network is constructed to solve the problems of numerous connection and complicated management in the tissue culture room, and the control system based on STM8S single chip microcomputer and REX3U wireless module is designed to realize the light intensity and the light quality can be adjusted. The silicon photocell is selected as the photodetector of illumination detection module, which is used to monitor the change of light environment. The module is composed of LED light source and constant current drive circuit. (2) the development of system software, including communication protocol, PC interface, lower computer execution procedures. According to the requirement of ZigBee wireless module, the communication transmission protocol is established, and the whole structure and function module of the upper computer operating interface are written with C # language, which is convenient for users to manage the tissue culture room, and C language is used to write the programs of each module of the lower computer. Complete signal reception, analysis and processing. (3) based on the photometric principle, the relationship between light intensity and photosynthetic effective quantum flux density is established, and its correctness is verified by experiments. The results show that the relative error between the experimental value and the theoretical value is 3.86 ~ 2.66, and the control error of photosynthetic effective quantum flux density is less than 5%. The system can provide maximum illumination intensity of 90 渭 mol m-2s 1. Under the condition of 65 渭 mol m-2s1 light intensity, the maximum ratio of red and blue light intensity is 12: 1. (4) using the developed light system, the culture experiment of Dendrobium candidum tissue culture seedling was carried out under different light quality. The results showed that the effects of light quality RB5: 1 on adventitious bud number, seedling height, node number and Internode length of Dendrobium candidum were the most obvious, but the effects of different light quality on dry matter and chlorophyll accumulation were not significant.
【学位授予单位】：中国计量学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：TM923.34;Q943.1

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