生物电解池氨氧化脱氮产能

发布时间：2018-07-03 17:43

本文选题：生物电解池 + 氨氧化　；参考：《应用与环境生物学报》2014年06期

【摘要】：以不锈钢筒作为阴极、碳毡作为阳极,阴阳极间利用无纺布作为隔膜构建单室生物电解池,以氨作为唯一电子供体,接入混合菌群,通过恒定不同的阳极电势,考察不同初始浓度氨氮在生物电解池内的氧化与产物的生成情况.结果表明,恒定阳极电势0.2 V(vs Ag/Ag Cl)时,经过5 d的运行,初始氨氮浓度200 mg/L、400 mg/L的氨氮去除率分别为30%、35%,氮气分别积累16.1 m L、17.18 m L,甲烷分别积累1.18 m L、1.46 m L;恒定阳极电势0.6 V(vs Ag/Ag Cl)时,初始氨氮浓度200 mg/L、400 mg/L,氨氮去除率分别为32.4%、36.6%,分别积累氮气16.48 m L、17.42 m L,积累甲烷1.3m L、1.52 m L,未检测到硝态氮和亚硝态氮.循环伏安扫描分析发现,阳极具有明显的氧化还原峰,且不同的阳极恒定电势,导致其氧化还原峰出现偏移.通过电镜扫描,发现阳极微生物细胞表面具有明显的褶皱形状,高通量分析显示阳极微生物中Geobacter占24.11%,是优势菌群,在阳极氨氧化过程中起到关键作用.同时发现系统中还存在氢营养型产甲烷菌Synergistes(3.8%)以及梭菌Clo stridium(3.8%)和Gordonia(1.85%)等功能微生物.本文研究表明,在生物电解池内,微生物能够以氨氮作为电子供体,通过氨氧化脱氮,并产生氢气和甲烷.
[Abstract]:Using stainless steel tube as cathode, carbon felt as anode, non-woven cloth as diaphragm between cathode and anode, single chamber biological electrolysis cell was constructed. Ammonia was used as sole electron donor, and mixed microflora was connected, and different anode potential was constant. The oxidation of ammonia nitrogen with different initial concentrations and the formation of products in the bioglytic cell were investigated. The results show that the constant anode potential is 0. 2 V (vs Ag/Ag Cl) and runs for 5 days. The initial NH3-N concentration of 200mg / L ~ (400mg / L) was 300.35%, the nitrogen accumulation was 16.1mL ~ (-17.18) mL, the methane concentration was 1.18mL / L ~ (1.46 mL), and the constant anode potential was 0.6V (vs Ag/Ag Cl). The initial ammonia nitrogen concentration was 200 mg / L ~ 400 mg 路L ~ (-1), the removal rate of ammonia nitrogen was 36.4%, the nitrogen accumulation was 16.48 mL / L ~ 17.42 mL, the accumulation of methane was 1.3 m L ~ (-1) ~ 1.52 mL, no nitrate nitrogen and no nitrite nitrogen were detected. Cyclic voltammetry analysis shows that the anode has obvious redox peak and different constant potential of the anode, which results in the deviation of the redox peak. Through scanning electron microscope, it was found that the surface of anodic microorganism had obvious fold shape. High-throughput analysis showed that Geobacter accounted for 24.11% of the anodic microorganism, which was the dominant microflora and played a key role in the anodic ammonia oxidation process. It was also found that functional microorganisms such as Synergistes (3.8%), Clo stridium (3.8%) and Gordonia (1.85%) were found in the system. In this paper, it is shown that in the biological electrolysis cell, the microorganism can use ammonia nitrogen as the electron donor to denitrification through ammonia oxidation, and produce hydrogen and methane.
【作者单位】：四川大学生命科学学院;中国科学院成都生物研究所;
【基金】：国家自然科学基金项目(31270166)资助~~
【分类号】：X703;TM911.45

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