激光共焦显微光束的偏转扫描

发布时间：2018-03-11 21:43

本文选题：共焦显微　切入点：二维振镜　出处：《光学精密工程》2016年06期 　论文类型：期刊论文

【摘要】：为了提高激光共聚焦系统的扫描速度,本文提出一种逐场扫描的场同步扫描方法。构建了激光共焦显微系统,将美国THORLABS公司的GVS002型二维检流计振镜应用于该系统,根据光学系统参数以及扫描范围要求计算振镜的整场扫描波形。借助NI公司的PCIe6353多功能数据采集卡,输出行同步的扫描波形,同时,对共焦显微系统共焦位置上针孔处的光强信号进行采集,先后扫描一幅256×256和512×512的图像,记录扫描图像和成像时间;然后,在相同的硬件结构下,以场同步的方式输出扫描波形,记录扫描图像和成像时间。实验结果表明:场同步方式扫描256×256图像的速度可提高10倍,扫描512×512图像的速度可提高5倍,且满足共焦显微成像的清晰、抗干扰能力强等要求。与行同步扫描方法相比,场同步扫描方法可以消除行与行之间转换的停留时间,在不改变硬件的情况下大幅提高扫描速度。
[Abstract]:In order to improve the scanning speed of laser confocal system, a field-by-field scanning method is proposed in this paper. A laser confocal microscopy system is constructed. The GVS002 type 2-D galvanometer of THORLABS Company is applied to the system. According to the requirements of optical system parameters and scanning range, the whole field scanning waveform of vibrating mirror is calculated. With the help of NI company's PCIe6353 multi-function data acquisition card, the scanning waveform of line synchronization is output, at the same time, The intensity signal of the pinhole in the confocal microscopy system is collected, a 256 脳 256 and 512 脳 512 images are scanned successively, the scanning image and imaging time are recorded, and the scanning waveform is output in the same hardware structure in a field synchronous manner. The experimental results show that the speed of 256 脳 256 images can be increased by 10 times, the speed of scanning 512 脳 512 images can be increased by 5 times, and the confocal microscopic imaging is clear. Compared with the line synchronous scanning method, the field synchronous scanning method can eliminate the residence time of line to line conversion, and improve the scanning speed without changing the hardware.
【作者单位】：北京理工大学光电学院;
【基金】：国家自然基金优秀青年科学基金资助项目(No.51422501);国家自然基金面上项目(No.61475020)
【分类号】：TH742;TN249

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