福建沿海米氏凯伦藻赤潮对皱纹盘鲍鳃组织抗氧化酶活性的影响研究

发布时间：2018-05-19 17:46

本文选题：米氏凯伦藻(Karenia + mikimotoi)　；参考：《海洋科学》2016年06期

【摘要】：本文初步研究了在较低赤潮密度(低于107个/m L)下,一株米氏凯伦藻(Karenia mikimotoi)对皱纹盘鲍(Haliotis discus hannai)鳃内几种抗氧化酶,如超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)的毒性效应,以期分析米氏凯伦藻对鲍鱼生命活动可能的胁迫方式。研究表明,米氏凯伦藻对SOD、CAT酶活性均造成不利影响,并未对GSH-Px酶造成不利影响,其酶活性显著高于对照组。米氏凯伦藻对SOD酶活性的影响表现为"先诱导后抑制"效应,24 h内,各处理组(0.1个/m L,0.5个/m L,1.0×104个/m L)中,酶活性急剧增高,分别是对照组的1.2,1.3,1.3倍,之后酶活性迅速下降,分别是对照组的77%,77%,73%。SOD能够清除机体体内多余的自由基,其活力变化反映了机体抵制自由基损伤能力已受到明显抑制。此外,米氏凯伦藻处理组中,CAT酶活性则处于"被抑制"状态,48 h内酶活性持续下降,分别降低至对照组的58%,51%,37%。CAT可以清除SOD歧化超氧阴离子自由基产生的H2O2,其活力的下降也可能造成机体内过氧化物的累积及氧化损伤。结果表明,即使未达到较高赤潮密度(不超过107个/m L)时,米氏凯伦藻短时间内仍可对鲍鱼鳃内关键抗氧化酶活性造成显著抑制效应,这极有可能导致鲍鱼机体抗氧化系统遭受严重损伤。
[Abstract]:In this paper, at low red tide density (below 107 / mL), a strain of Karenia mikimotoi has been used to study several kinds of antioxidase in the Gill of Haliotis discus hannaie. For example, the toxic effects of superoxide dismutase (SOD), catalase (catalase), glutathione peroxidase (Glutathione peroxidase) (Glutathione peroxidase) The results showed that the activity of GSH-Px cat was significantly higher than that of the control. The effect of Karen's algae on SOD activity was as follows: within 24 h, the enzyme activity of each treatment group (0.1 / mL, 0.5 / mL = 1.0 脳 10 ~ 4 / mL) was significantly higher than that of the control group (1.2 ~ 1.3 ~ (1.3) times of the control group), and then the enzyme activity was decreased rapidly in each treatment group (0.1 / mL, 0.5 / mL, 1.0 脳 104 脳 10 ~ (4) / mL), respectively. The activity of SOD in the control group can eliminate the excess free radicals in the body, which reflects that the ability of resisting free radical damage has been obviously inhibited. In addition, the activity of catalase in the treatment group of Karen Michaelis was "inhibited" and decreased continuously within 48 h. Cat can scavenge the H _ 2O _ 2 produced by the superoxide anion radical of SOD disproportionation, and the decrease of its activity may also lead to the accumulation of peroxide and oxidative damage in the body. The results showed that, even if the red tide density was not higher (less than 107 / mL), the activity of key antioxidant enzymes in the gills of abalone could be inhibited significantly in a short period of time. This is likely to cause severe damage to the antioxidant system of abalone.
【作者单位】：中国科学院海洋研究所海洋生态与环境科学重点实验室;中国环境科学研究院流域水生态保护技术研究室;
【基金】：国家自然科学基金项目(41476102) 中国科学院战略重点研究项目(XDA01020304)~~
【分类号】：X55

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