海洋石油降解菌的筛选及复合菌系的构建
发布时间：2019-03-31 09:48
【摘要】：为采用生物法治理海洋石油污染,以原油为唯一碳源,从深圳海域5个采样点取样,通过富集、涂布平板分离高效石油降解菌,并以复配、正交等方式构建石油降解复合菌系;通过生理生化实验和16S r RNA基因序列分析对菌株进行鉴定;采用单因素实验对复合菌系降解石油的条件进行优化,并使用气相色谱-质谱法(GC-MS)研究其对石油的降解特性。结果显示,共分离得到22株高效石油降解菌,对石油的降解率为34.5%-52.2%;由S1-30、S1-38和S2-13构建了复合菌系SQ1,对石油降解率可达68.3%,3株菌分别鉴定为棒杆菌(Corynebacterium sp.)、迪茨氏菌(Dietzia sp.)和拉布伦茨氏菌(Labrenzia sp.);经优化,SQ1在最适条件下(30℃、p H7.6、石油浓度20 g/L),11 d内对石油的去除率高达73.5%;GC-MS结果表明,复合菌系SQ1对石油总烷烃的去除率为91.7%,对较难降解的C21-C35烷烃组分的降解率接近100%。研究表明,复合菌系SQ1在海洋石油污染的生物修复中具有较强的应用潜力。
[Abstract]:In order to treat marine oil pollution by biological method, crude oil was used as the sole carbon source and sampled from five sampling sites in Shenzhen sea area. High efficiency petroleum degrading bacteria were separated by enrichment and coated plate, and the petroleum degradation complex bacteria were constructed by means of compound and orthogonal methods. The strain was identified by physiological and biochemical experiments and 16s r RNA gene sequence analysis. Single factor experiments were used to optimize the degradation conditions of the complex bacteria, and gas chromatography-mass spectrometry (GC-MS) was used to study the degradation characteristics of petroleum. The results showed that 22 strains of high efficiency oil degrading bacteria were isolated and the oil degradation rate was 34.5% / 52.2%. The oil degradation rate of SQ1, was up to 68.3%, and the three strains were identified as Corynebacterium (Corynebacterium sp.), (Dietzia sp.), which was constructed from S1 ~ (30), S _ (1) ~ (38) and S _ (2) ~ (13). Under the optimum conditions (30 鈩，
本文编号：2450796
[Abstract]:In order to treat marine oil pollution by biological method, crude oil was used as the sole carbon source and sampled from five sampling sites in Shenzhen sea area. High efficiency petroleum degrading bacteria were separated by enrichment and coated plate, and the petroleum degradation complex bacteria were constructed by means of compound and orthogonal methods. The strain was identified by physiological and biochemical experiments and 16s r RNA gene sequence analysis. Single factor experiments were used to optimize the degradation conditions of the complex bacteria, and gas chromatography-mass spectrometry (GC-MS) was used to study the degradation characteristics of petroleum. The results showed that 22 strains of high efficiency oil degrading bacteria were isolated and the oil degradation rate was 34.5% / 52.2%. The oil degradation rate of SQ1, was up to 68.3%, and the three strains were identified as Corynebacterium (Corynebacterium sp.), (Dietzia sp.), which was constructed from S1 ~ (30), S _ (1) ~ (38) and S _ (2) ~ (13). Under the optimum conditions (30 鈩，
本文编号：2450796
本文链接：https://www.wllwen.com/kejilunwen/haiyang/2450796.html
