基于移动式3D微流控纸芯片的功能化研究

发布时间：2018-06-29 23:42

本文选题：纸芯片 + 回形针　；参考：《烟台大学》2017年硕士论文

【摘要】：微流控纸芯片是一种新型的简易分析设备,它将微流控技术的微型化与纸质材料的廉价易得等优点相结合,发展成为当前备受关注的热点领域,在环境监测,食品质量控制以及生物医学等方面都呈现出巨大的应用潜力。本文主要研究一种新型的3D纸芯片制作方法,同时设计了两种流体控制方式,并对制作的纸芯片进行了功能化研究。本论文可以分为两个部分:第一部分为综述。首先,介绍了纸芯片的优点,加工、检测方法以及应用等;其次,介绍了3D纸芯片的研究进展,重点突出了其加工方法及流体控制方法;最后,阐述了酶联免疫反应在纸芯片方面的具体应用。第二部分为研究报告,主要由三章构成,具体内容如下:第一章,建立一种新型的3D微流控纸芯片制作技术-回形针固定法。设计多种不同纸芯片,并对回形针方法的重现性、纸芯片的通道及性能进行考察。利用自主研制的纸芯片比色检测装置,对牛血清白蛋白(BSA)、二价铁离子进行定量检测。在所规定的范围内,都获得了较好的线性关系。同时,我们将回形针固定法与其他几种方法进行了比较,证明回形针固定法的可行性。第二章,基于前面回形针固定法的基础上,设计一种新的控制流体流动的方法-移动阀,并实现了对癌症标志物癌胚抗原(CEA)的比色免疫检测。首先,利用蜡印法制作纸芯片,并将纸芯片和移动阀相结合,制作了多种形式的移动阀纸芯片,介绍了其工作原理并对其性能进行探究;其次,将比色酶联免疫分析与移动阀纸芯片相结合,构建了微流控纸芯片比色免疫传感器,根据显色信号的强度,对CEA的浓度进行定量分析。同时,利用这种方法检测了实际血清中的CEA,验证移动阀纸芯片的可行性。第三章,主要研究一种新的圆形的旋转阀纸芯片,并将纸芯片与辣根过氧化物酶(HRP)催化的鲁米诺化学发光免疫体系结合,建立一种在纸芯片上检测血清中癌症标识物甲胎蛋白(AFP)的新方法,同时搭建了一种简单的化学发光检测设备。基于AFP上标记的HRP能有效催化luminol-H2O2化学发光体系的化学发光,根据化学发光信号强度与AFP浓度存在的关系,实现对甲胎蛋白的即时检测。结果表明,二者呈现良好的线性关系,同时,利用此方法检测了实际血清中的AFP,证明了旋转阀纸芯片的可操作性。
[Abstract]:Microfluidic paper chip is a new type of simple analysis equipment. It combines the advantages of microfluidic technology and cheap and easy to obtain paper materials. Food quality control and biomedicine have great application potential. In this paper, a new method of making 3D paper chip is studied, two kinds of fluid control methods are designed, and the function of the paper chip is studied. This paper can be divided into two parts: the first part is a summary. Firstly, the advantages, processing, testing methods and applications of paper chip are introduced. Secondly, the research progress of 3D paper chip is introduced, especially its machining method and fluid control method. The application of enzyme-linked immunoreactivity (Elisa) in paper chip was described. The second part is the research report, which consists of three chapters. The main contents are as follows: in chapter 1, a new 3D microfluidic paper chip fabrication technique, paper clip fixing method, is established. Several different paper chips were designed, and the reproducibility of paper clip method, the channel and performance of paper chip were investigated. Bovine serum albumin (BSA) and divalent iron ions were quantitatively detected by a paper chip colorimetry device developed by ourselves. A good linear relationship is obtained within the prescribed range. At the same time, the paper clip fixation method is compared with other methods to prove the feasibility of paper clip fixation method. In the second chapter, a new method to control the flow of fluid, the moving valve, is designed, and the colorimetric detection of carcinoembryonic antigen (CEA), a cancer marker, is realized. Firstly, the paper chip is made by wax printing method, and the paper chip and the moving valve are combined to make various kinds of moving valve paper chip. The working principle of the chip is introduced and its performance is explored. A microfluidic paper chip colorimetric immunosensor was constructed by combining colorimetric enzyme linked immunoassay (Elisa) with moving valve paper chip. The concentration of CEA was quantitatively analyzed according to the intensity of color signal. At the same time, this method was used to detect the CEA in real serum to verify the feasibility of mobile valve paper chip. In chapter 3, a new circular rotary valve paper chip was studied, and the paper chip was combined with the luminol chemiluminescence immune system catalyzed by horseradish peroxidase (HRP). A new method for the detection of alpha-fetoprotein (AFP) in serum on paper chip was established, and a simple chemiluminescence detection equipment was built. HRP labeled with AFP can effectively catalyze the chemiluminescence of luminol-H2O2 chemiluminescence system. According to the relationship between the intensity of chemiluminescence signal and the concentration of AFP, the real-time detection of alpha-fetoprotein can be realized. The results show that there is a good linear relationship between them. Meanwhile, AFP in serum is detected by this method, which proves the maneuverability of rotary valve paper chip.
【学位授予单位】：烟台大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ056.16

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