稀土掺杂的纳米晶的合成与生物成像应用的研究
发布时间：2018-08-19 06:06
【摘要】：稀土掺杂的纳米晶由于其独特的发光性质,在生物成像和生物探针领域有着非常广阔的应用前景。但稀土纳米晶本身对于待检测离子不具有响应性,需要和有机小分子相结合。构建稀土纳米晶和有机小分子的复合体系,进行活体内的近红外发光探针的研究,是目前研究的热点。本文通过在稀土纳米晶表面引入介孔二氧化硅壳层作为载体,将有机小分子染料载入介孔二氧化硅壳层,设计合成了近红外光激发的活体次氯酸根检测的纳米探针,应用于小动物活体内的炎症模型的检测。为了进一步提高穿透深度,我们合成了Nd3+掺杂的稀土下转换纳米晶,用于活体的肝脾和淋巴结的光学和磁共振双模式成像。1、上转换纳米体系用于体外和体内次氯酸的灵敏检测我们设计了Er3+、Tm3+共掺杂的稀土上转换发光纳米粒子、介孔二氧化硅壳层和h Cy5.5有机小分子染料构成的三层复合体系,用于HOCl/Cl O-的检测。并将这一体系用于细胞和活体水平上的HOCl/Cl O-检测。然后建立了昆明鼠(带毛)关节炎的炎症模型,将材料用于小鼠关节炎的检测,实现了细胞和活体内的上转换成像和比度成像。2、PEG修饰的Na YF4:Nd@Na Gd F4纳米晶用于下转换和磁共振双模成像的研究Nd3+掺杂的稀土下转换纳米晶相比于980 nm激发的上转换纳米粒子有着更高的穿透深度和比较低的热效应。我们合成了不同Nd3+掺杂比的Na YF4:Nd,并在外层包覆一层Na Gd F4壳层,然后筛选出了发光性质最优的核壳结构掺杂比。并将核壳结构的Na YF4:Nd@Na Gd F4纳米晶用亲水性的PEG修饰过以后,用于小鼠的肝脾成像和荧光指导淋巴结切除手术。由于有Na Gd F4壳层的包覆,材料能够用于磁共振成像,实现了荧光和磁共振的双模成像。
[Abstract]:Rare earth doped nanocrystals have a broad application prospect in the field of biological imaging and biological probe due to their unique luminescent properties. However, rare earth nanocrystals are not responsive to the ions to be detected and need to be combined with small organic molecules. It is a hot topic to construct the composite system of rare earth nanocrystalline and organic small molecule and study the near infrared luminescence probe in vivo. In this paper, the mesoporous silica shell was introduced on the surface of rare earth nanocrystals as the carrier, and the organic small molecular dyes were loaded into the mesoporous silica shell layer. A near-infrared photoexcited nano-probe for the detection of hypochlorite was designed and synthesized. It is applied to the detection of inflammatory model in vivo in small animals. In order to further improve the penetration depth, Nd3 doped rare earth downconversion nanocrystals were synthesized. Optical and Magnetic Resonance Dual-Mode Imaging for liver-spleen and lymph nodes in Vivo. The up-conversion nanocrystalline system was used for sensitive detection of hypochloric acid in vitro and in vivo. We designed Er3 Tm3 co-doped rare-earth up-conversion luminescent nanoparticles. A three-layer composite system composed of mesoporous silica shell and h Cy5.5 organic small molecular dyes was used for the detection of HOCl/Cl O-. The system was used for the detection of HOCl/Cl O-at the cellular and in vivo levels. Then the inflammatory model of Kunming mouse (hairy) arthritis was established, and the material was used for the detection of mouse arthritis. Up-conversion of cells and in vivo and specific imaging of PEG-modified Na YF4:Nd@Na GdF4 nanocrystals for downconversion and magnetic resonance dual-mode imaging; Nd3 doped rare-earth downconversion nanocrystals compared to 980nm excited upconversion Nanoparticles have higher penetration depth and lower thermal effects. We synthesized NaYF4: Ndwith different doping ratios of Nd3 and coated a layer of NaGdF4 shell with the outer layer. Then we screened out the optimum doping ratio of core-shell structure for luminescent properties. Na YF4:Nd@Na Gd F 4 nanocrystals with core-shell structure were modified with hydrophilic PEG for liver and spleen imaging and fluorescence guided lymph node excision in mice. Because of the coating of Na Gd F 4 shell, the material can be used in magnetic resonance imaging, and the fluorescence and magnetic resonance double mode imaging can be realized.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ133.3;TB383.1
本文编号：2190866
[Abstract]:Rare earth doped nanocrystals have a broad application prospect in the field of biological imaging and biological probe due to their unique luminescent properties. However, rare earth nanocrystals are not responsive to the ions to be detected and need to be combined with small organic molecules. It is a hot topic to construct the composite system of rare earth nanocrystalline and organic small molecule and study the near infrared luminescence probe in vivo. In this paper, the mesoporous silica shell was introduced on the surface of rare earth nanocrystals as the carrier, and the organic small molecular dyes were loaded into the mesoporous silica shell layer. A near-infrared photoexcited nano-probe for the detection of hypochlorite was designed and synthesized. It is applied to the detection of inflammatory model in vivo in small animals. In order to further improve the penetration depth, Nd3 doped rare earth downconversion nanocrystals were synthesized. Optical and Magnetic Resonance Dual-Mode Imaging for liver-spleen and lymph nodes in Vivo. The up-conversion nanocrystalline system was used for sensitive detection of hypochloric acid in vitro and in vivo. We designed Er3 Tm3 co-doped rare-earth up-conversion luminescent nanoparticles. A three-layer composite system composed of mesoporous silica shell and h Cy5.5 organic small molecular dyes was used for the detection of HOCl/Cl O-. The system was used for the detection of HOCl/Cl O-at the cellular and in vivo levels. Then the inflammatory model of Kunming mouse (hairy) arthritis was established, and the material was used for the detection of mouse arthritis. Up-conversion of cells and in vivo and specific imaging of PEG-modified Na YF4:Nd@Na GdF4 nanocrystals for downconversion and magnetic resonance dual-mode imaging; Nd3 doped rare-earth downconversion nanocrystals compared to 980nm excited upconversion Nanoparticles have higher penetration depth and lower thermal effects. We synthesized NaYF4: Ndwith different doping ratios of Nd3 and coated a layer of NaGdF4 shell with the outer layer. Then we screened out the optimum doping ratio of core-shell structure for luminescent properties. Na YF4:Nd@Na Gd F 4 nanocrystals with core-shell structure were modified with hydrophilic PEG for liver and spleen imaging and fluorescence guided lymph node excision in mice. Because of the coating of Na Gd F 4 shell, the material can be used in magnetic resonance imaging, and the fluorescence and magnetic resonance double mode imaging can be realized.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ133.3;TB383.1
【参考文献】
相关期刊论文 前2条
1 李艳红;张永明;马晶;陆海燕;马铭远;;GdF_3∶Eu~(3+)/NaGdF_4∶Eu~(3+)纳米晶的水热合成及发光性质[J];无机化学学报;2011年06期
2 ;Preparation and luminescence properties of monodisperse silica/aminosilane-coated Y_2O_3:Yb,Ho upconversion nanoparticles[J];Chinese Science Bulletin;2011年02期
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