盐度对SBR和SBBR工艺短程硝化反硝化的影响

发布时间：2017-12-31 07:21

本文关键词：盐度对SBR和SBBR工艺短程硝化反硝化的影响　出处：《中国海洋大学》2015年硕士论文　论文类型：学位论文

【摘要】：本论文利用序批式活性污泥反应器(SBR)和序批式生物膜反应器(SBBR),研究了盐度变化对污染物去除和短程硝化反硝化稳定性的影响。(1)用全程硝化污泥为接种污泥,在盐度0.6%条件下控制供气量(360 mL/min)和反应温度(30℃),经3个月启动时间两个反应器内均实现了高效短程硝化反硝化。进水盐度从1.0%逐步升高到5.0%时,盐度的提高未对两个反应器内COD的去除产生明显抑制作用。进水盐度在1.0%-4.5%范围时,两个反应器出水NH4+-N、NO2--N和N03--N分别小于1.0,0.5,0.5 mg/L,盐度的提高未对两个反应器的硝化过程和反硝化过程产生抑制作用；在此盐度范围内,SBR中亚硝酸盐积累率(NAR)均在97.00%以上,SBBR中NAR均在95.00%以上。进水盐度提高到5.0%时,SBR和SBBR分别运行9d和7d后,NH4+-N的氧化过程几乎被完全抑制,短程硝化被彻底破坏。(2)进水盐度从0.6%逐步升高到5.0%时,SBR和SBBR中污泥容积指数(SVI)随盐度的增加先增加后降低。盐度在1.0%-3.0%范围时,SBR活性污泥、SBBR中悬浮污泥和生物膜的微生物活性比耗氧速率(SOUR)均随盐度的提高逐渐降低。盐度在1.0%-5.0%范围时,三种来源微生物的比氨氧化速率(SAOR)、比亚硝酸盐还原速率(SNIRR)和比硝酸盐还原速率(SNARR)均随盐度的增加出现不同程度的下降。盐度增加对三种来源微生物的SAOR抑制程度均大于对SNIRR和SNARR的抑制程度,说明NO2--N和N03--N还原菌相比硝化细菌具有更强的耐盐能力。此盐度范围内,三种来源微生物的SNIOR均未检测出,盐度为1.0%-3.0%范围时,未检测出是因为短程消化稳定性未遭到破坏；盐度为5.0%时,未检测出是因为硝化反应被抑制。各盐度条件下,SBBR悬浮污泥微生物活性均高于生物膜上微生物活性。(3)微生物胞外聚合物(EPS)分析结果表明,SBR活性污泥、SBBR中悬浮污泥及生物膜的可溶性微生物产物(SMP)、松散附着型EPS (LB-EPS)和紧密附着型EPS (TB-EPS)中的蛋白质(PN)、多糖(PS)都随盐度的提高先增加后降低,均在盐度为2.0%条件下达到最大值。盐度在0.6%-5.0%范围时,三种来源微生物的SMP、LB-EPS和TB-EPS样品中均出现4个荧光峰,2个与类蛋白荧光有关,2个与类腐殖酸荧光有关。随着盐度的增加,三种来源微生物的LB-EPS样品中类腐殖酸荧光有明显变化,说明盐度的增加对LB-EPS中腐殖酸的组成和结构有明显的影响。SBBR中悬浮污泥EPS的类腐殖酸荧光峰变化比生物膜明显(主要表现在LB-EPS).据EPS的傅里叶红外光谱分析结果,盐度在0.6%-5.0%范围时,三种来源微生物的SMP、LB-EPS和TB-EPS红外光谱吸收峰相似,但吸收峰的相对振动强度随盐度的增加而变化。
[Abstract]:The sequencing batch reactor activated sludge using the (SBR) and sequencing batch biofilm reactor (SBBR), studied the effect of salinity on pollutant removal and denitrification stability. (1) with complete nitrification sludge as seed sludge, gas supply in 0.6% salinity control (360 mL/min) and the reaction temperature (30 DEG C), starting 3 months two reactors have achieved efficient nitrification and denitrification. The influent salinity gradually increased from 1% to 5%, the increase of salinity of two reactor COD removal had obvious inhibition. The salinity in the range of 1.0%-4.5%, two the reactor effluent NH4+-N, NO2--N and N03--N were less than 1.0,0.5,0.5 mg/L, inhibited the increase of salinity is not produced during the nitrification process of two reactors and denitrification; the salinity range of SBR, nitrite accumulation rate (NAR) were more than 97% S. BBR NAR was above 95%. The water salinity increased to 5%, SBR and SBBR respectively, 9D and 7d after operation, the oxidation process of NH4+-N was almost completely inhibited, nitrification was completely destroyed. (2) the water salinity gradually increased to 5% from 0.6%, the sludge volume index SBR and SBBR (SVI) with the increase of salinity increases first and then decreases. The salinity in the range of 1.0%-3.0% SBR, activated sludge, suspended sludge and biofilm SBBR in microbial activity than the oxygen consumption rate (SOUR) decreased with the increase of salinity decreased gradually. The salinity in the range of 1.0%-5.0%, three kinds of microorganism than ammonia oxidation rate (SAOR), ratio of nitrite the reduction rate (SNIRR) and the ratio of nitrate reduction rate (SNARR) increased with the increase of salinity decreased to different extent. The salinity increase on the three sources of microbial inhibition of SAOR were significantly higher than the degree of inhibition of SNIRR and SNARR, NO2--N and N03--N that reducing bacteria Compared with the salt resistant ability of nitrifying bacteria is stronger. The salinity range, three sources of microbial SNIOR were not detected, the salinity range of 1.0%-3.0%, was not detected because of short digestion stability is not destroyed; the salinity of 5%, was not detected because denitrification should be suppressed. The salinity conditions suspended sludge, microbial activity of SBBR was higher than the activity of microbial biofilm. (3) microbial extracellular polymers (EPS) analysis showed that SBR activated sludge, soluble microbial products suspended sludge and biofilm in SBBR (SMP), loose adhesion type EPS (LB-EPS) and EPS (TB-EPS) closely attached. The protein (PN), polysaccharides (PS) with the increase of salinity increased first and then decreased, in 2% salinity conditions. Salinity reached the maximum value in the range of 0.6%-5.0%, three sources of microbial SMP, 4 fluorescence peaks appeared in the sample of LB-EPS and TB-EPS 2, and protein like fluorescence, 2 with humic fluorescence. With the increase of salinity, three sources of microbial samples of LB-EPS humic acid fluorescence changes obviously, indicating the increase of salinity of LB-EPS in the composition and structure of humic acid with humic like fluorescence peak changes of suspended sludge EPS.SBBR obviously than in biofilm (mainly in LB-EPS). According to the Fourier transform infrared spectroscopy EPS analysis results, the salinity in the range of 0.6%-5.0%, three sources of microbial SMP, LB-EPS and TB-EPS peak of infrared spectrum is similar, but the relative intensity of vibration absorption peak increased with salinity changes.

【学位授予单位】：中国海洋大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X703

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