厌氧产乙酸菌株ZY-3的筛

发布时间：2018-01-05 03:26

本文关键词：厌氧产乙酸菌株ZY-3的筛选、鉴定及产酸条件　出处：《江苏农业科学》2016年12期 　论文类型：期刊论文

【摘要】：为筛选高效厌氧水解产乙酸菌,将其应用于高浓度有机废水的厌氧生物处理,强化产乙酸菌群的产乙酸作用,提高消化效率。采用Hungate厌氧操作技术以及选择性培养基分离筛选高效产乙酸菌,经生态学、生理生化、16S r DNA序列同源性分析对菌株的发酵产酸特性进行分析鉴定。结果表明,从污水处理厂活性污泥中分离出1株厌氧水解产乙酸菌ZY-3,经鉴定为双酶梭状芽孢杆菌(Clostridium bifermentans)。该菌株在以麦芽糖为碳源、初始pH值为7.5、30℃温度下培养12 h,乙酸产量最高达1 346.7 mg/L;在添加0.4 mol/L NaCl的发酵培养基中可正常生长且产酸量较0、0.2 mol/L NaCl处理增加,高达1 588.7 mg/L。菌株ZY-3产酸能力强,发酵性能稳定,还能利用丙酸盐发酵产乙酸,对加速乙酸转化、防止丙酸积累、提高厌氧消化效率具有重要意义。
[Abstract]:For the screening of acetic acid bacteria producing anaerobic hydrolysis, anaerobic biological treatment is applied to high concentration organic wastewater, strengthen the production of acetic acid bacteria producing acetic acid, improve the digestion efficiency. Medium screening, acetogen, using the Hungate anaerobic technology and selectivity by ecology, physiology and biochemistry, 16S R DNA sequence analysis of fermentation characteristics of the strains were analyzed and identified. The results showed that the isolated 1 strains of anaerobic hydrolysis to produce acetic acid bacteria ZY-3 from activated sludge in the sewage treatment plant, was identified as Clostridium bifermentans (Clostridium bifermentans). The strain in maltose as carbon source, initial pH value of the temperature of 7.5,30 DEG C cultured for 12 h, acetic acid production reached 1346.7 mg/L; 0.4 mol/L NaCl added in the fermentation culture of normal growth and acid production than 0,0.2 mol/L NaCl increased in the base, up to 1588.7 mg/ L. strain ZY-3 producing acid It has strong capacity, stable fermentation performance, and can also produce acetic acid by propionate fermentation, which is of great significance for accelerating acetic acid transformation, preventing propionic acid accumulation and improving anaerobic digestion efficiency.

【作者单位】：河北省科学院生物研究所;河北大学生命科学学院;河北师范大学;
【基金】：河北省科技支撑计划(编号:14222901D;14273801D) 河北省财政预算(编号:15302) 河北省青年基金(编号:C2015302018)
【分类号】：X172;X703
【正文快照】： 高浓度有机废水的厌氧生物处理技术因具有有效、简单、费用低廉等特点而被全世界广泛应用[1]。然而在厌氧工艺中仍有诸多不足之处,例如由于厌氧细菌增殖较慢而导致的厌氧反应器初次启动过程缓慢、出水挥发性脂肪酸(volatilefatty acid,简称VFA)含量较高、大量丙酸积累导致系统

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