基于亚甲基蓝的水体急性毒性快速检测方法研究

发布时间：2018-01-06 13:05

本文关键词：基于亚甲基蓝的水体急性毒性快速检测方法研究　出处：《分析化学》2016年09期 　论文类型：期刊论文

【摘要】：水体急性毒性的快速检测对保障用水安全至关重要。本研究以荧光假单胞菌为毒性检测的受试体,以细胞膜受损为毒性响应机理,建立了一种新颖的基于亚甲基蓝的水体急性毒性快速检测方法。在本方法中,亚甲基蓝可以对细胞膜受损的细胞进行染色,而不能对细胞膜结构完整的细胞进行染色,因此,当有毒性物质存在并对细胞膜产生破坏时,溶液中的亚甲基蓝会进入细胞对细胞进行染色,从而降低溶液中的亚甲基蓝浓度。通过光谱法检测溶液中亚甲基蓝浓度的变化,就可以实现对水体急性毒性的快速检测。实验结果表明,本方法对3,5-二氯苯酚(DCP)、As~(3+)和Hg~(2+)的最低响应浓度分别为1.6,12.5和3.2 mg/L,对DCP的最低响应浓度比商业化仪器Baroxymeter低。本方法操作简单,检测快速,结果可靠,在水体急性毒性的检测和监测领域具有很好的应用前景。
[Abstract]:The rapid detection of acute toxicity in water is very important to ensure the safety of water. In this study, Pseudomonas fluorescein was used as the toxicity test subject, and the damage of cell membrane was taken as the toxic response mechanism. A novel rapid method for the detection of acute toxicity of water based on methylene blue was developed. In this method methylene blue can be used to stain damaged cells. Cells with intact cell membrane structure can not be stained, so when toxic substances exist and damage the cell membrane, methylene blue in the solution will enter the cell to stain the cell. In order to reduce the concentration of methylene blue in the solution, the change of methylene blue concentration in the solution can be detected by spectrum method, and the rapid detection of the acute toxicity of water can be realized. The experimental results show that the method is effective for 3. The minimum response concentrations of 5-dichlorophenol (DCPP) and Hg~(2) were 1. 610. 5 and 3. 2 mg/L, respectively. The minimum response concentration of DCP is lower than that of commercial instrument Baroxymeter. The method is simple, rapid and reliable. It has a good application prospect in the field of detection and monitoring of acute toxicity in water.
【作者单位】：中国水产科学研究院;中国科学院长春应用化学研究所电分析化学国家重点实验室;
【基金】：国家自然科学基金(Nos.31301177,21405148) 科技部重大科学仪器专项(No.2013YQ17058501) 吉林省科技厅(No.20150520010JH)资助~~
【分类号】：R123.1
【正文快照】： 1引言近年来,水质污染日益严重,越来越多的毒性物质流入水体,对生物产生各种各样的毒性效应[1]。因此,为了保障用水安全必须实现水体急性毒性的快速检测。传统的水体毒性检测方法采用了精确的终点生物化验法,例如:死亡率、生长率等。采用的受试体则包括高等动物浮游生物(D.mag

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