人工光源补光对沉水植物净化染料废水效果及其生理影响研究

发布时间：2018-01-08 15:08

本文关键词：人工光源补光对沉水植物净化染料废水效果及其生理影响研究　出处：《南京师范大学》2015年硕士论文　论文类型：学位论文

【摘要】：沉水植物广泛应用于污染水体的生态修复,对水质具有良好的净化作用。针对染料废水常规物化和生化处理方法的不足以及沉水植物应用于染料废水净化存在的水下光照不足问题,提出了人工光源辅助沉水植物净化染料废水的新方法,通过调控人工光源的光质、光强和日补光时间等参数,改善沉水植物的生境,提高沉水植物的净化能力,为进一步拓宽沉水植物的应用和发展染料废水治理新技术提供依据。论文系统研究了人工光源补光对典型沉水植物净化染料废水效果和生理变化的影响,主要研究内容和研究结果如下：(1)实验研究了红、蓝、白三种光质人工光源补光对典型沉水植物金鱼藻、苦草和菹草净化染料废水效果的影响,结果表明,三种光质的人工光源补光均可以提高金鱼藻、苦草和菹草的净化效果；红光对金鱼藻和苦草的污染净化能力的提高效果最佳,蓝光其次,白光最弱；而对于菹草,蓝光效果最好,红光次之,白光最弱；红光补光条件下的金鱼藻污染净化能力最强,停留时间5d时,可将废水的色度、总磷、氨氮由初始的40倍、1.5mg/L、8.0mg/L分别降至7倍、0.11mg/L、1.84mg/L,去除率分别可达到82.5%、92.7%、77.0%。(2)实验研究了光强和日补光时间对金鱼藻净化能力的影响,结果表明,实验范围内,光强的增加和日补光时间的延长与金鱼藻的污染净化能力呈正相关,然而考虑经济因素,适宜的工艺参数为：功率9w、补光3h、停留时间5d。(3)以小试确定的适宜工艺参数(红光、功率9w、补光3h),对某园区的染料生化尾水进行中试研究,结果表明,停留时间5d,染料生化尾水的色度、TP、氨氮、苯胺和COD的浓度可降至5倍、0.18mg/L、2.96mg/L、0.47mg/L和30.0mg/L,出水达到《城镇污水处理厂污染物排放标准》(GB18918—2002)的一级A标准,去除率分别可达到91.7%、90.3%、76.5%、83.5%和37.5%,处理效果稳定,说明人工光源辅助沉水植物法是一种有效的染料废水处理技术,值得推广。(4)实验研究了红、蓝、白三种光质人工光源补光对金鱼藻的生理影响,结果表明,三种光质人工光源均可促使金鱼藻的SOD, CAT和POD酶对染料胁迫响应的提前和酶活性的提高,有利于提高植物抵抗胁迫的能力；其中,红光光源对酶活性响应的影响尤为突出,可以将CAT和POD的响应分别提前4.5d和2d,促进生物量的增长,是辅助沉水植物水体净化的最佳光质选择；实验范围内,光强的增加和日补光时间的延长均可以促进金鱼藻POD酶响应的提前,并且光强的增加和日补光时间的延长对植物MDA和叶绿素的含量基本无影响。(5)实验初步考察了金鱼藻提取过氧化物酶体的稳定性和对染料的降解机理,结果表明,金鱼藻提取过氧化物酶体具有较好的热稳定性、pH稳定性和耐藏性(45℃以下、pH=5-7、4℃冷藏6个月,活性仍能保持50%左右)。并且根据三维荧光和傅立叶红外分析结果,推测金鱼藻体内富含的过氧化物酶体可以降解大分子难降解的偶氮染料为小分子的芳香胺类物质。
[Abstract]:The ecological restoration of submerged macrophytes is widely used in polluted water purification, has good effect on water quality. Due to the shortage of conventional dye wastewater by physicochemical and biochemical treatment method and submerged plants used in dye wastewater purification of underwater light is insufficient, this paper puts forward a new method of dye wastewater purification aided submerged plant artificial light source, through the the regulation of artificial light, light quality, light intensity and light time and other parameters, improve the submerged plant habitat, improve the purification capacity of submerged plants, and provide a basis for the management of the new technology to further broaden the application and development of submerged plant dyestuff wastewater. This dissertation has systematically studied the effect of dye wastewater and physiological changes of artificial light fill purification of typical submerged the plant, the main research contents and results are as follows: (1) experimental study on red, blue and white three kinds of light and artificial light fill of typical submerged plant Physical effects, Ceratophyllum demersum, dye wastewater purification effect of Vallisneria natans and Potamogeton crispus showed that three kinds of artificial light fill light can improve the purification effect of Ceratophyllaceae, Vallisneria natans and Potamogeton crispus; purification ability of hornworts and Vallisneria red pollution and improve the effect of the best, blue, white and blue for the weakest; potamogetoncrispus, had the best effect. Red light, white light, red light under the condition of the weakest; Ceratophyllaceae pollution purification ability was the strongest, the residence time of 5D, the wastewater color, total phosphorus, ammonia nitrogen by 40 times, the initial 1.5mg/L, 8.0mg/L were reduced to 7 times, 0.11mg/L, 1.84mg/L removal rate can reach 82.5%, 92.7%, 77.0%. (2) experimental study on the effect of light intensity, and light time Ceratophyllum demersum purification ability. The results showed that within the experimental range extended with the increase of light intensity and light Ceratophyllaceae time pollution purification ability was positively correlated, however Considering the economic factors, the suitable technological parameters: power 9W, 3h light, the residence time of 5d. (3) to determine the optimum scale (red, light, power 9W, 3H) in a pilot study, park dye biochemical tail water in the results show that the residence time of 5D, dye color, biochemical material the tail water of TP, ammonia nitrogen, concentration of aniline and COD can be reduced to 5 times, 0.18mg/L, 2.96mg/L, 0.47mg/L and 30.0mg/L, the effluent can meet the discharge standard of pollutants "urban sewage treatment plant > (GB18918 - 2002) the level of A, the removal rate could reach 91.7%, 90.3%, 76.5%, 83.5% and 37.5%, the treatment effect stable, that artificial light assisted submerged plant is an effective technique of wastewater treatment, it is worthy of promotion. (4) experimental study on the effect of red, blue, white, three kinds of light physical artificial light to fill ceratophyllumdemersum. The results showed that three kinds of light and artificial light can promote the hornwort SOD, C AT and POD enzyme of stress response in advance and enzyme activity of dyes increased, is conducive to the improvement of plant resistance to stress; the red light effect on enzyme activity in response is particularly prominent, can be CAT and POD respectively in response to 4.5d and 2D, to promote the growth of biomass is the best light quality auxiliary submerged plant water purifying selection; experiment range, the increase of light intensity and light on the extension of time all can promote the early response of the hornwort POD enzyme, and the increase of light intensity and prolong the time to fill the basic content of plant chlorophyll MDA and shadowless ring. (5) the preliminary experimental investigation of stability of extraction and peroxisome hornwort dye the results show that the degradation mechanism, extraction of hornwort peroxisome has good thermal stability, pH stability and storability (below 45 DEG pH=5-7,4 DEG C, stored for 6 months, still can maintain activity About 50%). And according to the three-dimensional fluorescence and Fu Liye infrared analysis results, presumably in the body of peroxisomes can degrade hornwort macromolecules difficult degradation of azo dyes for aromatic amine molecules.

【学位授予单位】：南京师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X788;X173

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