海洋大型藻类对微藻类的化感作用

发布时间：2018-01-10 22:14

本文关键词：海洋大型藻类对微藻类的化感作用　出处：《大连海事大学》2015年硕士论文　论文类型：学位论文

【摘要】：海洋生物之间的关系错综复杂。海洋中不同种植物或微生物之间存在化感作用,利用化感作用治理赤潮,是一种新方法。它根据的是大型海藻对微藻有着化感抑制的作用。本研究选用三种大型海藻浸提液对两种微藻进行化感作用实验,研究其化感机理。测定的指标有：藻细胞计数、EC50值、丙二醛(MDA)含量、叶绿素a含量以及微藻碳氮稳定同位素的变化。各部分的实验结果为,三种大型海藻对塔玛亚历山大藻都有化感抑制的作用其中根据96h的EC50值以及微藻生长曲线来看,孔石莼对塔玛亚历山大藻的化感抑制作用最强。但孔石莼对青岛大扁藻主要表现为促进作用,说明青岛大扁藻的抗化感作用较强。大型海藻加入后,微藻体内MDA含量迅速升高,且受抑制越强,前期上升幅度也就越大。从叶绿素a含量可以看出,当微藻受到化感抑制作用时,它的叶绿素a含量要明显低于对照组,且大型海藻浸提液浓度越高,叶绿素a含量也就越低。从实验过程中塔玛亚历山大藻碳的同位素变化可以看出,孔石莼的加入,使得实验组中塔玛亚历山大藻的613C值先明显升高。且孔石莼的浓度越高,塔玛亚历山大藻的δ13C值在整个过程就越大。塔玛亚历山大藻各组中δ13N值都显示上升的趋势,且孔石莼浓度越高的实验组δ15N值就越小综上所述,大型海藻对微藻有化感作用,但同一种大型海藻对不同微藻的化感作用强弱不同。大型海藻对微藻化感抑制过程中,可以使微藻的MDA含量迅速升高,同时也使其叶绿素a含量降低。从微藻的C、N稳定同位素含量变化来看,化感作用也改变了微藻的C、N稳定同位素组成。
[Abstract]:The relationship between the marine organisms in the ocean. Perplexing between different plants or microorganisms exist allelopathy, the allelopathic effect of treating red tide, is a new method. It is based on the macroalgae have allelopathic effect on algae. This study selected three kinds of seaweed extracts on two kinds of microalgae the experimental study on Mechanism of allelopathy, the allelopathic index: Determination of algal cell count, EC50 value, malondialdehyde (MDA) content, chlorophyll a content and changes of microalgae of stable carbon and nitrogen isotope. Each part of the experimental results, three kinds of seaweeds on Tama Alexander Zao is allelopathic effect among them according to the 96h EC50 value and the microalgae growth curve, u.pertusa of Tamar Alexander allelopathic effect was the strongest. But u.pertusa in Qingdao mainly to promote macro algae, Qingdao macro algae allelopathic resistance Strong. Seaweed is added, the content of MDA in microalgae increased rapidly, and the inhibition is strong, early rising is also bigger. The content of chlorophyll a can be seen as affected by allelopathic effect of microalgae, the chlorophyll a content was significantly lower than that in control group, and the type of seaweed extract concentration the higher the chlorophyll a content was lower. The isotope variation of Tama Alexander algal carbon in the process of experiments can be seen with Ulva pertusa, the experimental group in the tama Alexander algae of 613C value increased significantly. And the higher the concentration of u.pertusa, Delta 13C Tamar Alexander algae in the whole process more value great. Show increased 13N values in each group and Tamar Alexander algae, Ulva pertusa concentration is higher in the experimental group 15N values is smaller in macroalgae have allelopathic effects on microalgae, but the same kind of seaweeds on different microalgae The allelopathic effects of different seaweeds on microalgae. Allelopathic inhibition process, can make the MDA content of microalgae increased rapidly, while the content of chlorophyll a decreased. The change of N C from microalgae, stable isotope content, allelopathy also changed the microalgae C N stable isotope composition.

【学位授予单位】：大连海事大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X55;X173

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