铀胁迫对反枝苋生理生化指标的影响分析

发布时间：2018-01-12 16:26

本文关键词：铀胁迫对反枝苋生理生化指标的影响分析　出处：《环境科学与技术》2016年11期 　论文类型：期刊论文

更多相关文章： 铀胁迫 反枝苋 植物修复 抗逆性

【摘要】：为探究铀胁迫下富集植物反枝苋的抗逆机制及铀对反枝苋的毒理效应,通过盆栽控制性试验,研究了土壤不同浓度铀(U)胁迫(0、25、50、100、200、400 mg//kg)对反枝苋(Amaranthus retroflexus L.)叶片光合色素、蛋白质、丙二醛、脯氨酸含量和抗氧化酶(POD、CAT和SOD)活性的影响。结果表明:土壤铀胁迫对反枝苋叶片光合色素和蛋白质含量表现出低促高抑,25 mg/kg处理下叶绿素a、叶绿素b和蛋白质含量显著高于对照;中低浓度下,抗氧化酶系统被激活,活性显著高于对照,有效清除活性氧自由基;100 mg/kg处理时,SOD活性率先降低,引起CAT和POD活性出现无底物性下降;200、400 mg/kg胁迫下植物产生并积累了大量的自由基,超过了SOD、CAT、POD的清除能力,致使酶活性降低,膜脂过氧化程度加深,MDA含量激增;各处理下脯氨酸含量均显著高于对照,使植物在高胁迫下抗氧化酶系统遭到破坏后仍能起到抗铀胁迫作用。
[Abstract]:In order to investigate the stress resistance of uranium toxicity a.retroflexus inverse mechanism and uranium enrichment plant Amaranthus retroflexus, by pot experiment, the effects of soil with different concentrations of uranium (U) stress (0,25,50100200400 mg//kg) of a.retroflexus (Amaranthus retroflexus L.) Ye Pianguang pigment, protein, MDA, proline content and antioxidant enzymes (POD, CAT and SOD) were studied. The results showed that soil uranium stress of Amaranthus retroflexus L. leaf photosynthetic pigment and protein content showed a high low concentration under 25 mg/kg inhibition, chlorophyll a, chlorophyll b and protein content was significantly higher than that of control; low concentration, anti oxidase system is activated that was significantly higher than the control, effectively remove oxygen free radicals; 100 mg/kg treatment, SOD activity decreased, resulting in CAT and POD activities decreased substrate; under the stress of 200400 mg/kg plants produced and accumulated a large number of free radicals Over SOD, CAT and POD scavenging capacity resulted in decreased enzyme activity, increased membrane lipid peroxidation, and increased MDA content. The proline content in all treatments was significantly higher than that in control, so that plants could still play an anti uranium stress role when the antioxidant enzyme system was destroyed under high stress.

【作者单位】：西南科技大学生命科学与工程学院;生物质材料教育部工程研究中心;西南科技大学核废物与环境安全国防重点学科实验室;
【基金】：国家核设施退役及放射性废物治理科研重点项目(14ZG6101)
【分类号】：X173;X591
【正文快照】： 《环境科学与技术》编辑部:(网址)http://fjks.chinajournal.net.cn(电话)027-87643502(电子信箱)hjkxyjs@vip.126.com随着国民经济的快速发展,核能在国防、工业、农业、医疗和科研中广泛应用的同时也伴随大量放射性废弃物的产生。我国放射性废弃物的年产量约为全国工业固体废

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