厌氧消化过程稳定性与微生物群落的相关性

发布时间：2018-01-13 14:22

本文关键词：厌氧消化过程稳定性与微生物群落的相关性　出处：《中国环境科学》2016年11期 　论文类型：期刊论文

【摘要】：为探析厌氧消化过程稳定性与微生物群落的相关性,在餐厨垃圾厌氧消化反应器中引入负荷扰动以诱导不同的运行状态,理化分析和高通量测序相结合用于研究各个状态下的状态参数响应及微生物群落动态.结果表明,均衡的群落结构保证了反应器的稳定运行,稳定状态下反应器的甲烷产率和挥发性固体(VS)去除率分别高达(0.50±0.01)LCH_4/g VS和(89.58±0.08)%.高负荷下产酸细菌(柔膜菌门、放线菌门)大量增殖,诱导互养脂肪酸降解菌(梭菌纲)的相对丰度剧增,然而与之互营的氢型产甲烷菌的丰度和活性却下降了.产甲烷菌与互养脂肪酸降解菌的失衡导致它们不能有效的互养合作,从而引起挥发性脂肪酸(VFA)积累和过程失稳.积累的VFA和氨使比乙酸产甲烷活性(SAMA)和比产甲烷活性(SMA)分别下降60.12%和72.51%,进一步加剧了过程失稳.扰动停止后,尽管反应器恢复了原有运行条件和性能,但微生物群落达到了新的平衡.
[Abstract]:In order to explore the relationship between the stability of anaerobic digestion process and microbial community, load disturbance was introduced into the anaerobic digestion reactor to induce different operating states. Physicochemical analysis combined with high-throughput sequencing was used to study the response of state parameters and microbial community dynamics in various states. The results showed that the balanced community structure ensured the stable operation of the reactor. The methane production rate and VSremoval efficiency of the reactor were as high as 0.50 卤0.01 LCH-4 / g vs and 89.58 卤0.08, respectively. Acidogenic bacteria under high load. Actinomycetes) proliferates in large quantities and induces the relative abundance of the biodegradable bacteria (Clostridium sp.). However, the abundance and activity of hydrogen methanogenic bacteria decreased. The imbalance between methanogenic bacteria and eutrophic fatty acid degrading bacteria made them unable to cooperate with each other effectively. Thus the accumulation and process instability of volatile fatty acids (VFAs) were caused by the accumulation of VFA and ammonia, which resulted in the methanogenic activity of acetic acid (SAMA) and specific methanogenic activity (SMA). It decreased by 60.12% and 72.51%, respectively. After the disturbance was stopped, the microbial community reached a new equilibrium, although the original operating conditions and performance were restored.
【作者单位】：重庆大学三峡库区生态环境教育部重点实验室;
【基金】：国家“十一五”科技支撑计划(2010BAC67B01)
【分类号】：X799;X172
【正文快照】： 我国餐厨垃圾产量逐年增加,处置不当会引发一系列环境卫生和食品安全问题.厌氧消化(AD)技术能在处理废物的同时回收能源,被广泛用于餐厨垃圾处理[1-2].然而AD系统在运行过程中易发生抑制、酸化、起泡等“过程不稳定”现象,在高负荷下运行时尤其显著[3-5].鉴于AD系统是以微生

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