油田采出液SRB生长特性及抑制方法研究

发布时间：2018-01-17 00:36

本文关键词：油田采出液SRB生长特性及抑制方法研究　出处：《东北石油大学》2015年硕士论文　论文类型：学位论文

【摘要】：硫化氢是毒性很强的气体,其危害有损害人类健康、污染环境、腐蚀设备等。鉴于吉林油田二氧化碳驱产生硫化氢的问题,本文探讨分析了硫化氢产生的原因,确定了硫酸盐还原菌的存在是产生硫化氢的根本原因,并进一步实验研究了硫酸盐还原菌的生长因素和抑制方法。通过对吉林油田各区块的普查,分析了集输系统中硫化氢浓度、硫酸盐还原菌菌数、硫酸根浓度、矿化度浓度和pH等,通过分析研究,集输系统中温度、pH、营养条件等总体适合硫酸盐还原菌的生长和代谢,故在集输系统中容易大量滋生硫酸盐还原菌,从而产生H2S气体,确定大量H2S的产生缘于集输过程中SRB的还原作用。随后对采集的水样进行分离提纯,得到纯硫酸盐还原菌菌株,富集培养,用富集到的菌液进行间歇实验,考察温度、pH、盐度、铁离子、碳源、重金属、二氧化碳等因素对硫酸盐还原菌生长的影响,确定其繁殖规律。进而研究SRB的抑制方法,主要从反硝化和杀菌剂两方面考虑,反硝化抑制硫酸盐还原菌的研究主要考察S/N、COD、碱度对反硝化细菌抑制硫酸盐还原菌效果的影响,实验结果表明,NO3-的加入可以有效的抑制SRB的生长及S2-的生成,含量越高抑制效果就越显著,COD含量较低时抑制效果更加明显,较高的碱度更有助于SRB的抑制。进一步对戊二醛、异噻唑啉酮、1227(十二烷基二甲基苄基氯化铵)及复配杀菌剂为实验对象考察杀菌效果,采用四试管简易绝迹稀释法确定各自的最低杀菌浓度,研究不同浓度不同时间的条件下各杀菌剂的杀菌效果,将复配杀菌剂和单一杀菌剂进行对比,选出最优杀菌剂。
[Abstract]:Hydrogen sulfide is a highly toxic gas, which is harmful to human health, pollutes the environment, corrodes equipment and so on. In view of the problem of hydrogen sulfide produced by carbon dioxide flooding in Jilin Oilfield, this paper discusses the causes of hydrogen sulfide production. It is determined that the existence of sulfate reducing bacteria is the fundamental cause of producing hydrogen sulfide, and the growth factors and inhibition methods of sulfate reducing bacteria are further studied. The concentration of hydrogen sulphide, the number of sulfate-reducing bacteria, the concentration of sulfate, the concentration of salinity and pH in the gathering and transportation system were analyzed. Nutrition conditions are suitable for the growth and metabolism of sulfate reducing bacteria, so it is easy to breed a large number of sulfate reducing bacteria in the gathering and transportation system, thus producing H2S gas. It was determined that a large amount of H _ 2S was produced by the reduction of SRB during gathering and transportation. Then the collected water samples were separated and purified to obtain the pure sulfate reducing bacteria strain for enrichment and culture. The effects of temperature, pH, salinity, iron ion, carbon source, heavy metal and carbon dioxide on the growth of sulfate reducing bacteria were studied. Then the inhibition methods of SRB were studied mainly from the aspects of denitrification and fungicides, and the inhibition of sulfate reducing bacteria by denitrification was mainly investigated. The effect of alkalinity on the inhibition effect of denitrifying bacteria on sulfate reducing bacteria. The experimental results showed that the addition of no _ 3- could effectively inhibit the growth of SRB and the formation of S ~ (2-). The higher the content of denitrifying bacteria was, the more significant the inhibition effect was. The inhibition effect of COD was more obvious when the content of COD was lower, and the higher basicity was more helpful to the inhibition of SRB, and further to glutaraldehyde and isothiazolone. The bactericidal effect of 1227 (12 alkyl dimethyl benzyl ammonium chloride) and compound fungicides was investigated. The minimal bactericidal concentration was determined by simple extinction dilution method in four test tubes. The germicidal effects of various fungicides with different concentration and time were studied. The optimum bactericides were selected by comparing the compound fungicides and single fungicides.
【学位授予单位】：东北石油大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X741

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