谷胱甘肽引导合成金属纳米颗粒及其检测重金属离子的应用

发布时间：2018-01-17 12:58

本文关键词：谷胱甘肽引导合成金属纳米颗粒及其检测重金属离子的应用　出处：《天津大学》2015年硕士论文　论文类型：学位论文

【摘要】：一些重金属离子,例如Hg~(2+)、Ag~+、Pb~(2+)、Cd~(2+)、As~(3+)等可通过食物链在人体内富集,最终引起肾衰竭,神经性疾病,心脏、心血管以及肺部损伤等疾病。因此,重金属检测方法的研究具有非常重要的意义。相比电化学检测,荧光检测等方法,比色法具有检测效果明显可见等优势。然而,其较高的检测限阻碍了比色检测的发展。本文利用谷胱甘肽(GSH)为模板合成了具有较高过氧化物酶活性的GSH-Pt NPs以及GSH-Pd NPs,并通过比色法将其应用于重金属离子检测。首先,利用GSH合成了粒径分布在2.3-3.6 nm的Pt纳米颗粒,纳米颗粒表面电子结构与[K_2PtCl_4]/[GSH]相关。[K_2PtCl_4]/[GSH]=6(GSH-Pt3.3)包含67%Pt0以及33%Pt~(2+),其过氧化物酶活性最高,[K_2PtCl_4]/[GSH]=60(GSH-Pt3.6)包含82%Pt0以及18%Pt~(2+)具有最高的自由基消除能力。由于Hg~(2+)能够抑制过氧化物酶活性,一系列不同粒径的Pt纳米颗粒用于Hg~(2+)检测,其中GSH-Pt3.6对汞离子检测限可达到0.25 nM。Pt纳米酶同时在饮用水、湖水以及血清中Hg~(2+)检测表现出良好的应用前景。其次,通过GSH为模板合成了GSH-Pd纳米颗粒,[Na2PdCl4]/[GSH]=6(GSH-Pd2.6)比例下合成的纳米颗粒其过氧化物酶活性最高。对于[Na2PdCl4]/[GSH]=60(GSH-Pd3.5)纳米颗粒,其零价Pd与正价Pd含量分别为72.5%和27.5%,随着GSH-Pd纳米颗粒粒径的减小,其零价Pd含量亦逐渐下降。纳米颗粒粒径由2.6减小至1.4时其零价Pd含量由56.7%减小为30.4%。GSH-Pd2.6用于检测Ag~+,其检测限为1.14 n M,线性区间为0-100 nM。
[Abstract]:Some heavy metal ions such as Hg~(2 can enrich human body through the food chain and eventually lead to renal failure. Neurologic diseases, heart, cardiovascular and lung damage. Therefore, the study of heavy metal detection methods has a very important significance, compared with electrochemical detection, fluorescence detection and other methods. Colorimetric method has the advantages of visible detection effect. The high detection limit hinders the development of colorimetry. GSH-Pt NPs and GSH-Pd NPs with high peroxidase activity were synthesized as templates. At first, Pt nanoparticles with particle size distribution of 2.3-3.6 nm were synthesized by GSH. [K_2PtCl_4] /. [GSH. [K_2PtCl_4] /. [GSH (GSH-Pt3.3) contains 67Pt0 and 33Pt0, and its peroxidase activity is the highest. [K_2PtCl_4] /. [(GSH) contains 82Pt0 and 18Pt2) has the highest free radical elimination ability. Because of Hg~(2). Can inhibit the activity of peroxidase. A series of Pt nanoparticles with different diameters were used for Hg~(2) detection, in which the detection limit of mercury ions by GSH-Pt3.6 could reach 0.25 nM.Pt nanonuclease simultaneously in drinking water. The detection of Hg~(2 in lake water and serum showed a good application prospect. Secondly, GSH-Pd nanoparticles were synthesized by using GSH as template. [Na2PdCl4] /. [The peroxidase activity of the synthesized nanoparticles was the highest at the ratio of GSH to GSH-Pd2.6. [Na2PdCl4] /. [The content of zero valence PD and positive value PD were 72.5% and 27.5, respectively, and the particle size of GSH-Pd nanoparticles decreased with the decrease of GSH-Pd nanoparticles. The content of zero valence PD decreased from 56.7% to 30.4. GSH-Pd2.6 was used for the determination of Ag2 ~ when the particle size decreased from 2. 6 to 1. 4. The detection limit is 1.14 nm and the linear range is 0-100 nm.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X830;O657.3

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