固相萃取-高效液相色谱-串联质谱测定水中5种大环内酯类抗生素

发布时间：2018-01-24 05:26

本文关键词： 大环内酯类抗生素固相萃取高效液相色谱-串联质谱水体　出处：《中国测试》2017年03期 　论文类型：期刊论文

【摘要】：建立固相萃取(SPE)、高效液相色谱-串联质谱(HPLC-MS/MS)同时测定水中5种大环内酯类抗生素(罗红霉素、克拉霉素、阿奇霉素、螺旋霉素和泰乐菌素)的分析方法。水样中的抗生素通过Oasis HLB固相萃取小柱富集后,以C18反相色谱柱为分析柱,甲醇和0.1%甲酸水溶液为流动相,采用HPLC-MS/MS进行定量分析,检测方式为多反应监测模式(MRM),内标法定量。5种大环内酯类抗生素的回收率在64.8%~90.8%之间,仪器检出限为0.012~0.085μg/L,定量限为0.041~0.285μg/L。将建立的方法用于检测某化学工业园区周边地表水中5种目标分析物的残留,均未检测到泰乐菌素,其余4种抗生素均有不同程度检出,检出浓度在ng/L水平。
[Abstract]:A solid phase extraction (SPE) method was established for the simultaneous determination of five macrolides (roxithromycin, clarithromycin, azithromycin) in water by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS). Analysis method of spiramycin and tylosin. The antibiotics in the water samples were enriched by Oasis HLB solid phase extraction column, and the column was C18 reversed phase chromatography. Methanol and 0.1% formic acid aqueous solution were used as mobile phase for quantitative analysis by HPLC-MS/MS. The recoveries of 5 macrolides by internal standard method were between 64.8% and 90.8%, and the detection limit of the instrument was 0.012 ~ 0.085 渭 g / L. The quantitative limit was 0.041 ~ 0.285 渭 g 路L ~ (-1). The established method was used to detect the residues of five target analytes in the surface water around a chemical industrial park, and no tylosin was detected. The other four antibiotics were detected to some extent, and the detected concentration was at the level of ng/L.
【作者单位】：苏州农业职业技术学院环境工程学院;南京大学环境学院污染控制与资源化研究国家重点实验室;
【基金】：国家863课题(2013AA06A309) 苏州农业职业技术学院青年教师科研能力提升计划资助项目(PPS201304)
【分类号】：O657.63;X832
【正文快照】： 学结构,抗生素主要分为喹诺酮类、β-内酰胺类、氯霉素类、磺胺类、大环内酯类、头孢菌素类、四环素类、氨基糖苷类[1]。大环内酯类抗生素(macrolides an-tibiotics,MLs)对治疗因支原体、衣原体、流感嗜血杆菌以及立克次体等引起的疾病都特别有效,不仅能用于对人类的治疗,也可

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