两株硫酸盐还原菌的培养条件研究

发布时间：2018-02-14 04:06

  本文关键词： 硫酸盐还原菌 培养条件 菌剂　出处：《四川农业大学学报》2016年04期 　论文类型：期刊论文

【摘要】：【目的】研究两株SRB菌生长繁殖的影响因素,进行SRB菌剂的初步试制。【方法】采用CFU法进行菌株密度计数,考察不同p H、培养温度、碳源、培养基等条件对两株SRB菌株生长的影响,并对SRB4菌株进行了菌剂的初步试制。【结果】菌株SRB4的最适培养条件:温度为38℃;起始p H为8.0;培养基是以柠檬酸钠为碳源的改良Postgate培养基。菌株SRB3的最适培养条件:温度为36℃;起始p H为7.0;培养基是以玉米芯水提液为碳源的Postgate培养基。经菌剂初步试制,SRB4菌株在发酵48 h后菌株密度保持在1012 CFU/m L以上。【结论】两株SRB菌在最适培养条件下密度保持在1012 CFU/m L以上,可作为工程应用的备选菌株。
[Abstract]:[objective] to study the factors affecting the growth and reproduction of two strains of SRB, and to make a preliminary trial production of SRB. [methods] the density of two strains of SRB was counted by CFU method, and the different pH, culture temperature and carbon source were investigated. The effects of culture medium on the growth of two SRB strains were studied, and the preliminary preparation of SRB4 strain was carried out. [results] the optimum culture conditions for SRB4 were as follows: temperature 38 鈩，

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