利用文蛤生物标志物评价尾水-海水混合体系污染水平

发布时间：2018-02-24 06:23

本文关键词： 尾水市政污水处理厂海水综合评价双壳类动物生物标志物　出处：《中国环境科学》2016年09期 　论文类型：期刊论文

【摘要】：采用不同浓度(体积比0%~40%,经天然海水稀释而成)的市政污水处理厂(MSTP)尾水对文蛤(Meretrix meretrix)连续培养9d,通过测定9种生化因子水平评价污染暴露对双壳类的生物学效应包括:内脏中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽还原酶(GR)、谷胱甘肽(GSH)、乙酰胆碱酯酶(ACh E)、金属硫蛋白(MTs)、硫代巴比妥酸反应物(TBARs)以及血细胞的溶酶体膜稳定性(LMS).SOD、CAT除外的所有生化因子均能对尾水污染产生敏感响应,其中,GPx、MTs、ACh E和LMS的响应与尾水浓度呈显著相关(P0.05或P0.01),适于作为尾水污染的生物标志物;依据这些生物标志物计算的综合生物标志物响应指数值(IBR_(v2)=0.61~2.65)随尾水浓度上升而增大,且两者间存在显著相关性(P0.01).研究结果表明,基于生物标志物响应值计算IBR指数的方法适于MSTPs尾水-海水混合体系的综合污染评价.
[Abstract]:Meretrix Meretrix of Meretrix Meretrix was cultured in municipal sewage treatment plant (MSTP) with different concentrations (volume ratio 0: 40, diluted by natural seawater) for 9 days. The biological effects of pollution exposure on bivalves were evaluated by measuring the levels of 9 biochemical factors. These include: superoxide dismutase (SOD) in the viscera, catalase (catalase), glutathione peroxidase (Glutathione peroxidase), glutathione reductase (Glutathione), glutathione glutathione (GSH), acetylcholinesterase (ach), metallothionein (MTS), thiobarbituric acid (TBARs), and. The stability of lysosomal membrane of hemocytes and all biochemical factors except LMSG. SOD cat were sensitive to tail water pollution. There was significant correlation between the response of LMS and LMS and the concentration of tail water, P 0.05 or P0.01, which was suitable as a biomarker of tail water pollution, and the response index of comprehensive biomarker calculated from these biomarkers was increased with the increase of the concentration of tail water, and the value of response index of comprehensive biomarker based on these biomarkers was 0.61 ~ 2.65). The results showed that the method of calculating IBR index based on the response value of biomarker was suitable for the comprehensive pollution evaluation of MSTPs tail-sea water mixture system.
【作者单位】：中国海洋大学海洋环境与生态教育部重点实验室;
【基金】：国家自然科学基金项目(41240040)
【分类号】：X703;X55

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